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Article

Silencing TGIF suppresses migration, invasion and metastasis of MDA‑MB‑231 human breast cancer cells

  • Authors:
    • Yadong Wang
    • Li Li
    • Haiyu Wang
    • Jiangmin Li
    • Haiyan Yang
  • View Affiliations / Copyright

    Affiliations: Department of Toxicology, Henan Center for Disease Control and Prevention, Zhengzhou, Henan 450016, P.R. China, Department of Epidemiology, School of Public Health, Zhengzhou University, Zhengzhou, Henan 450001, P.R. China
  • Pages: 802-808
    |
    Published online on: December 4, 2017
       https://doi.org/10.3892/or.2017.6133
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Abstract

This study explored the potential role of TG-interacting factor (TGIF) in migration, invasion and metastasis of the human breast cancer cell line MDA‑MB‑231. Western blot assay, immunohistochemistry and qRT-PCR assays were applied to detect the expression of protein and mRNA. Wound healing assay, Transwell invasion assay and tail vein metastatic assay were performed to assess the migration, invasion and metastasis of stable TGIF-silenced MDA‑MB‑231 cell line in vitro and in vivo. The significantly higher frequency of TGIF high-expression was observed in metastatic breast cancer (62.9%) compared to that in non-metastatic breast cancer (25.8%). Silencing TGIF suppressed migration and invasion of MDA‑MB‑231 cells in vitro and tumor metastasis in nude mouse models. The expression of Snail1, matrix metalloproteinase 2 (MMP2) and β-catenin was markedly decreased in the stable TGIF-silenced MDA‑MB‑231 cells compared with the control cells. Our results suggest that silencing TGIF suppressed the migration, invasion and metastasis of the human breast cancer cell line MDA‑MB‑231 using in vitro and in vivo experiments.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Li L, Wang H, Li J and Yang H: Silencing TGIF suppresses migration, invasion and metastasis of MDA‑MB‑231 human breast cancer cells. Oncol Rep 39: 802-808, 2018.
APA
Wang, Y., Li, L., Wang, H., Li, J., & Yang, H. (2018). Silencing TGIF suppresses migration, invasion and metastasis of MDA‑MB‑231 human breast cancer cells. Oncology Reports, 39, 802-808. https://doi.org/10.3892/or.2017.6133
MLA
Wang, Y., Li, L., Wang, H., Li, J., Yang, H."Silencing TGIF suppresses migration, invasion and metastasis of MDA‑MB‑231 human breast cancer cells". Oncology Reports 39.2 (2018): 802-808.
Chicago
Wang, Y., Li, L., Wang, H., Li, J., Yang, H."Silencing TGIF suppresses migration, invasion and metastasis of MDA‑MB‑231 human breast cancer cells". Oncology Reports 39, no. 2 (2018): 802-808. https://doi.org/10.3892/or.2017.6133
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Li L, Wang H, Li J and Yang H: Silencing TGIF suppresses migration, invasion and metastasis of MDA‑MB‑231 human breast cancer cells. Oncol Rep 39: 802-808, 2018.
APA
Wang, Y., Li, L., Wang, H., Li, J., & Yang, H. (2018). Silencing TGIF suppresses migration, invasion and metastasis of MDA‑MB‑231 human breast cancer cells. Oncology Reports, 39, 802-808. https://doi.org/10.3892/or.2017.6133
MLA
Wang, Y., Li, L., Wang, H., Li, J., Yang, H."Silencing TGIF suppresses migration, invasion and metastasis of MDA‑MB‑231 human breast cancer cells". Oncology Reports 39.2 (2018): 802-808.
Chicago
Wang, Y., Li, L., Wang, H., Li, J., Yang, H."Silencing TGIF suppresses migration, invasion and metastasis of MDA‑MB‑231 human breast cancer cells". Oncology Reports 39, no. 2 (2018): 802-808. https://doi.org/10.3892/or.2017.6133
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