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Article

Overexpression of interleukin-32α promotes invasion by modulating VEGF in hepatocellular carcinoma

  • Authors:
    • Wen-Bo Zhao
    • Quan-Li Wang
    • Yan-Tian Xu
    • Shi-Feng Xu
    • Yang Qiu
    • Feng Zhu
  • View Affiliations / Copyright

    Affiliations: Department of Hematology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China, Department of Traditional Chinese Medicine, County People's Hospital of Wulian, Rizhao, Shandong 262300, P.R. China, Department of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China, Department of Hematology and Oncology, Affiliated Hospital of Taishan Medical University, Taian, Shandong 27100, P.R. China
  • Pages: 1155-1162
    |
    Published online on: December 19, 2017
       https://doi.org/10.3892/or.2017.6162
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Abstract

Interleukin-32α (IL-32α) was reported to exhibit pluripotent pro-inflammatory properties. Recent studies indicate that it promotes the migration and invasion of cancers. We detected the expression of IL-32 in hepatocellular carcinoma (HCC) tissues and investigated its role in tumor angiogenesis and invasion. IL-32α expression in HCC was evaluated by real-time PCR, western blot analysis and immunohistochemical (IHC) staining. Secreted serum IL-32α and VEGF concentrations were detected using a custom-made sandwich ELISA. Furthermore, IL-32α was knocked down in HCC cell lines using siRNA and the cell migration and invasion abilities were assessed. IHC staining showed that IL32α-positive particles were mainly located in the cytoplasm of cancer cells, and it was significantly upregulated in the tumor tissues compared with that in peritumoral tissues. Notably, IL-32α was strongly expressed in perivascular areas. The mean serum concentration of IL-32α in HCC patients was significantly higher than that in the control group (571.45±102.28 vs. 144.60±51.172 pg/ml; P<0.01). Real-time RT-PCR showed that IL-32α mRNA was significantly overexpressed in HCC tumor tissues (IL-32/β-actin, 15.59±7.8 vs. 3.37±0.47; P<0.01). The in vitro results indicated that IL-32α knockdown inhibited the activation of VEGF-STAT3 signaling in HCC tumor cell lines. IL-32α expression was correlated with clinical relevance in HCC tumor tissues. It is strongly suggested that IL-32α may be a potential predictor of anti-angiogenesis therapy and prognosis of HCC.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao W, Wang Q, Xu Y, Xu S, Qiu Y and Zhu F: Overexpression of interleukin-32α promotes invasion by modulating VEGF in hepatocellular carcinoma. Oncol Rep 39: 1155-1162, 2018.
APA
Zhao, W., Wang, Q., Xu, Y., Xu, S., Qiu, Y., & Zhu, F. (2018). Overexpression of interleukin-32α promotes invasion by modulating VEGF in hepatocellular carcinoma. Oncology Reports, 39, 1155-1162. https://doi.org/10.3892/or.2017.6162
MLA
Zhao, W., Wang, Q., Xu, Y., Xu, S., Qiu, Y., Zhu, F."Overexpression of interleukin-32α promotes invasion by modulating VEGF in hepatocellular carcinoma". Oncology Reports 39.3 (2018): 1155-1162.
Chicago
Zhao, W., Wang, Q., Xu, Y., Xu, S., Qiu, Y., Zhu, F."Overexpression of interleukin-32α promotes invasion by modulating VEGF in hepatocellular carcinoma". Oncology Reports 39, no. 3 (2018): 1155-1162. https://doi.org/10.3892/or.2017.6162
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao W, Wang Q, Xu Y, Xu S, Qiu Y and Zhu F: Overexpression of interleukin-32α promotes invasion by modulating VEGF in hepatocellular carcinoma. Oncol Rep 39: 1155-1162, 2018.
APA
Zhao, W., Wang, Q., Xu, Y., Xu, S., Qiu, Y., & Zhu, F. (2018). Overexpression of interleukin-32α promotes invasion by modulating VEGF in hepatocellular carcinoma. Oncology Reports, 39, 1155-1162. https://doi.org/10.3892/or.2017.6162
MLA
Zhao, W., Wang, Q., Xu, Y., Xu, S., Qiu, Y., Zhu, F."Overexpression of interleukin-32α promotes invasion by modulating VEGF in hepatocellular carcinoma". Oncology Reports 39.3 (2018): 1155-1162.
Chicago
Zhao, W., Wang, Q., Xu, Y., Xu, S., Qiu, Y., Zhu, F."Overexpression of interleukin-32α promotes invasion by modulating VEGF in hepatocellular carcinoma". Oncology Reports 39, no. 3 (2018): 1155-1162. https://doi.org/10.3892/or.2017.6162
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