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Article

MicroRNA-let-7a regulates cell autophagy by targeting Rictor in gastric cancer cell lines MGC-803 and SGC-7901

  • Authors:
    • Hao Fan
    • Mingkun Jiang
    • Bowen Li
    • Yu He
    • Chi Huang
    • Dakui Luo
    • Hao Xu
    • Li Yang
    • Jundong Zhou
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China, The Core Laboratory of The Suzhou Cancer Center and Department of Radiotherapy, Nanjing Medical University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215001, P.R. China
  • Pages: 1207-1214
    |
    Published online on: January 5, 2018
       https://doi.org/10.3892/or.2018.6194
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Abstract

miR-let-7a is the most widely studied miRNA, whose functions have been well-established by scientists in both carcinogenesis and progression of human cancer, including gastric cancer (GC). However, to date there is a lack of information concerning the relationship between miR-let-7a and cellular autophagy. Using western blotting and immunofluorescence, we determined that upregulation of miR-let-7a led to increased cellular autophagic level, whereas miR-let-7a suppression decreased autophagy activity in GC cells. To further elucidate the mechanisms underlying this, we screened potential targets of miR-let-7a using bioinformatics analyses, validated by a series of assays. Our results indicated that Rptor independent companion of mTOR complex 2 (Rictor) was a direct target of miR-let-7a. In addition, rescue experiments in vitro showed that miR-let-7a promoted cellular autophagic level by inhibiting Rictor expression in GC cells. Furthermore, as an upstream executor of Akt-mTOR signaling pathway, we found that Rictor elaborated its effect on autophagy by phosphorylating Akt and mTOR, and this regulatory process could also be mediated by miR-let-7a. Taken together, our results present a novel role for miR-let-7a in GC which modulates autophagy by targeting Rictor, following the regulation of Akt-mTOR signal pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Fan H, Jiang M, Li B, He Y, Huang C, Luo D, Xu H, Yang L and Zhou J: MicroRNA-let-7a regulates cell autophagy by targeting Rictor in gastric cancer cell lines MGC-803 and SGC-7901. Oncol Rep 39: 1207-1214, 2018.
APA
Fan, H., Jiang, M., Li, B., He, Y., Huang, C., Luo, D. ... Zhou, J. (2018). MicroRNA-let-7a regulates cell autophagy by targeting Rictor in gastric cancer cell lines MGC-803 and SGC-7901. Oncology Reports, 39, 1207-1214. https://doi.org/10.3892/or.2018.6194
MLA
Fan, H., Jiang, M., Li, B., He, Y., Huang, C., Luo, D., Xu, H., Yang, L., Zhou, J."MicroRNA-let-7a regulates cell autophagy by targeting Rictor in gastric cancer cell lines MGC-803 and SGC-7901". Oncology Reports 39.3 (2018): 1207-1214.
Chicago
Fan, H., Jiang, M., Li, B., He, Y., Huang, C., Luo, D., Xu, H., Yang, L., Zhou, J."MicroRNA-let-7a regulates cell autophagy by targeting Rictor in gastric cancer cell lines MGC-803 and SGC-7901". Oncology Reports 39, no. 3 (2018): 1207-1214. https://doi.org/10.3892/or.2018.6194
Copy and paste a formatted citation
x
Spandidos Publications style
Fan H, Jiang M, Li B, He Y, Huang C, Luo D, Xu H, Yang L and Zhou J: MicroRNA-let-7a regulates cell autophagy by targeting Rictor in gastric cancer cell lines MGC-803 and SGC-7901. Oncol Rep 39: 1207-1214, 2018.
APA
Fan, H., Jiang, M., Li, B., He, Y., Huang, C., Luo, D. ... Zhou, J. (2018). MicroRNA-let-7a regulates cell autophagy by targeting Rictor in gastric cancer cell lines MGC-803 and SGC-7901. Oncology Reports, 39, 1207-1214. https://doi.org/10.3892/or.2018.6194
MLA
Fan, H., Jiang, M., Li, B., He, Y., Huang, C., Luo, D., Xu, H., Yang, L., Zhou, J."MicroRNA-let-7a regulates cell autophagy by targeting Rictor in gastric cancer cell lines MGC-803 and SGC-7901". Oncology Reports 39.3 (2018): 1207-1214.
Chicago
Fan, H., Jiang, M., Li, B., He, Y., Huang, C., Luo, D., Xu, H., Yang, L., Zhou, J."MicroRNA-let-7a regulates cell autophagy by targeting Rictor in gastric cancer cell lines MGC-803 and SGC-7901". Oncology Reports 39, no. 3 (2018): 1207-1214. https://doi.org/10.3892/or.2018.6194
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