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Article

Mechanism of ECM-induced dormancy and chemoresistance in A549 human lung carcinoma cells

  • Authors:
    • Siriporn Keeratichamroen
    • Kriengsak Lirdprapamongkol
    • Jisnuson Svasti
  • View Affiliations / Copyright

    Affiliations: Laboratory of Biochemistry, Chulabhorn Research Institute, Chulabhorn Graduate Institute, Bangkok 10210, Thailand
  • Pages: 1765-1774
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    Published online on: February 12, 2018
       https://doi.org/10.3892/or.2018.6258
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Abstract

It is now widely accepted that the tumor microenvironment influences the fate of cancer cells and plays crucial roles in regulating tumor dormancy and chemoresistance. The standard cell culture system on plastic surfaces does not account for cell interactions with the extracellular matrix (ECM), and is thus a less reliable approach to analyze cellular activity ex vivo. In the present study, A549 lung cancer cells were cultured in a semi-solid growth substrate (Matrigel) to mimic the tumor microenvironment and to investigate the role played by ECM proteins, as well as to evaluate the mechanism of cell-ECM communication. A549 cells embedded in semi-solid Matrigel exhibited dormant cell characteristics, including decreased cell proliferation, migration and invasion rates, compared with the corresponding cells cultured on plastic plates. Exposure of A549 cells to Matrigel leads to resistance against conventional chemotherapeutic drugs (etoposide, paclitaxel, vinblastine, doxorubicin and 2-deoxy-D-glucose). Cell cycle distribution analysis indicated that a larger percentage of the cells embedded within semi-solid Matrigel was arrested in the G0/G1 phase. RT-qPCR analysis revealed that A549 cells cultured in semi-solid Matrigel exhibited a marked decrease in the expression levels of genes that are related to tumor progression and invasion (uPA, uPAR, MMP2, MMP7, MMP9 and CXCR4). The effects of altering various signaling pathways, such as p-ERK, p-Akt and p-STAT3, were evaluated, in order to assess whether these pathways could account for the observed responses of the cells. The inhibition of ERK1/2 and Akt activation using specific inhibitors induced G0/G1 arrest and drug resistance. These results demonstrated that Matrigel drove A549 cells into a drug-resistant dormancy state, most likely through inhibition of the ERK1/2 and PI3K/Akt pathways. Cell culture within semi-solid Matrigel offered a simple in vitro model for studying the mechanisms responsible for tumor dormancy and drug resistance. These studies may lead to therapeutic approaches that can eliminate dormant tumor cells and prevent disease recurrence.
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View References

1 

Fridman R, Giaccone G, Kanemoto T, Martin GR, Gazdar AF and Mulshine JL: Reconstituted basement membrane (matrigel) and laminin can enhance the tumorigenicity and the drug resistance of small cell lung cancer cell lines. Proc Natl Acad Sci USA. 87:pp. 6698–6702. 1990; View Article : Google Scholar : PubMed/NCBI

2 

Weaver VM, Fischer AH, Peterson OW and Bissell MJ: The importance of the microenvironment in breast cancer progression: Recapitulation of mammary tumorigenesis using a unique human mammary epithelial cell model and a three-dimensional culture assay. Biochem Cell Biol. 74:833–851. 1996. View Article : Google Scholar : PubMed/NCBI

3 

Romero-López M, Trinh AL, Sobrino A, Hatch MM, Keating MT, Fimbres C, Lewis DE, Gershon PD, Botvinick EL, Digman M, et al: Recapitulating the human tumor microenvironment: Colon tumor-derived extracellular matrix promotes angiogenesis and tumor cell growth. Biomaterials. 116:118–129. 2017. View Article : Google Scholar : PubMed/NCBI

4 

Cramer GM, Jones DP, El-Hamidi H and Celli JP: ECM composition and rheology regulate growth, motility, and response to photodynamic therapy in 3D models of pancreatic ductal adenocarcinoma. Mol Cancer Res. 15:15–25. 2017. View Article : Google Scholar : PubMed/NCBI

5 

Lin CQ and Bissell MJ: Multi-faceted regulation of cell differentiation by extracellular matrix. FASEB J. 7:737–743. 1993. View Article : Google Scholar : PubMed/NCBI

6 

Streuli CH, Bailey N and Bissell MJ: Control of mammary epithelial differentiation: Basement membrane induces tissue-specific gene expression in the absence of cell-cell interaction and morphological polarity. J Cell Biol. 115:1383–1395. 1991. View Article : Google Scholar : PubMed/NCBI

7 

Muschler J, Lochter A, Roskelley CD, Yurchenco P and Bissell MJ: Division of labor among the alpha6beta4 integrin, beta1 integrins, and an E3 laminin receptor to signal morphogenesis and beta-casein expression in mammary epithelial cells. Mol Biol Cell. 10:2817–2828. 1999. View Article : Google Scholar : PubMed/NCBI

8 

Nakajima M, Morikawa K, Fabra A, Bucana CD and Fidler IJ: Influence of organ environment on extracellular matrix degradative activity and metastasis of human colon carcinoma cells. J Natl Cancer Inst. 82:1890–1898. 1990. View Article : Google Scholar : PubMed/NCBI

9 

Gohji K, Nakajima M, Boyd D, Dinney CP, Bucana CD, Kitazana S, Kamidono S and Fidler IJ: Organ-site dependence for the production of urokinase-type plasminogen activator and metastasis by human renal cell carcinoma cells. Am J Pathol. 151:1655–1661. 1997.PubMed/NCBI

10 

Luzzi KJ, MacDonald IC, Schmidt EE, Kerkvliet N, Morris VL, Chambers AF and Groom AC: Multistep nature of metastatic inefficiency: Dormancy of solitary cells after successful extravasation and limited survival of early micrometastases. Am J Pathol. 153:865–873. 1998. View Article : Google Scholar : PubMed/NCBI

11 

Naumov GN, MacDonald IC, Weinmeister PM, Kerkvliet N, Nadkarni KV, Wilson SM, Morris VL, Groom AC and Chambers AF: Persistence of solitary mammary carcinoma cells in a secondary site: A possible contributor to dormancy. Cancer Res. 62:2162–2168. 2002.PubMed/NCBI

12 

Naumov GN, Townson JL, MacDonald IC, Wilson SM, Bramwell VH, Groom AC and Chambers AF: Ineffectiveness of doxorubicin treatment on solitary dormant mammary carcinoma cells or late-developing metastases. Breast Cancer Res Treat. 82:199–206. 2003. View Article : Google Scholar : PubMed/NCBI

13 

Barkan D, Kleinman H, Simmons JL, Asmussen H, Kamaraju AK, Hoenorhoff MJ, Liu ZY, Costes SV, Cho EH, Lockett S, et al: Inhibition of metastatic outgrowth from single dormant tumor cells by targeting the cytoskeleton. Cancer Res. 68:6241–6250. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Boyerinas B, Zafrir M, Yesilkanal AE, Price TT, Hyjek EM and Sipkins DA: Adhesion to osteopontin in the bone marrow niche regulates lymphoblastic leukemia cell dormancy. Blood. 121:4821–4831. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Ghajar CM, Peinado H, Mori H, Matei IR, Evason KJ, Brazier H, Almeida D, Koller A, Hajjar KA, Stainier DY, et al: The perivascular niche regulates breast tumour dormancy. Nat Cell Biol. 15:807–817. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Oridate N, Lotan R and Lotan D: Reconstituted basement membrane (Matrigel): A useful semisolid medium for growth of tumor cell colonies. In Vitro Cell Dev Biol Anim. 32:192–193. 1996. View Article : Google Scholar : PubMed/NCBI

17 

Kleinman HK and Martin GR: Matrigel: Basement membrane matrix with biological activity. Semin Cancer Biol. 15:378–386. 2005. View Article : Google Scholar : PubMed/NCBI

18 

Alley MC, Scudiero DA, Monks A, Hursey ML, Czerwinski MJ, Fine DL, Abbott BJ, Mayo JG, Shoemaker RH and Boyd MR: Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Cancer Res. 48:589–601. 1988.PubMed/NCBI

19 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2ΔΔCT method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Meltzer A: Dormancy and breast cancer. J Surg Oncol. 43:181–188. 1990. View Article : Google Scholar : PubMed/NCBI

21 

Karrison TG, Ferguson DJ and Meier P: Dormancy of mammary carcinoma after mastectomy. J Natl Cancer Inst. 91:80–85. 1999. View Article : Google Scholar : PubMed/NCBI

22 

Aguirre-Ghiso JA: Models, mechanisms and clinical evidence for cancer dormancy. Nat Rev Cancer. 7:834–846. 2007. View Article : Google Scholar : PubMed/NCBI

23 

Yu W, Kim J and Ossowski L: Reduction in surface urokinase receptor forces malignant cells into a protracted state of dormancy. J Cell Biol. 137:767–777. 1997. View Article : Google Scholar : PubMed/NCBI

24 

Radinsky R: Modulation of tumor cell gene expression and phenotype by the organ-specific metastatic environment. Cancer Metastasis Rev. 14:323–338. 1995. View Article : Google Scholar : PubMed/NCBI

25 

Fidler IJ: Seed and soil revisited: Contribution of the organ microenvironment to cancer metastasis. Surg Oncol Clin N Am. 10:257–269. 2001.PubMed/NCBI

26 

Henriet P, Zhong ZD, Brooks PC, Weinberg KI and DeClerck YA: Contact with fibrillar collagen inhibits melanoma cell proliferation by up-regulating p27KIP1. Proc Natl Acad Sci USA. 97:pp. 10026–10031. 2000; View Article : Google Scholar : PubMed/NCBI

27 

Roth JM, Akalu A, Zelmanovich A, Policarpio D, Ng B, MacDonald S, Formenti S, Liebes L and Brooks PC: Recombinant alpha2(IV)NC1 domain inhibits tumor cell-extracellular matrix interactions, induces cellular senescence, and inhibits tumor growth in vivo. Am J Pathol. 166:901–911. 2005. View Article : Google Scholar : PubMed/NCBI

28 

Li X, Tsai P, Wieder ED, Kribben A, Van Putten V, Schrier RW and Nemenoff RA: Vascular smooth muscle cells grown on Matrigel. A model of the contractile phenotype with decreased activation of mitogen-activated protein kinase. J Biol Chem. 269:19653–19658. 1994.PubMed/NCBI

29 

Souter LH, Andrews JD, Zhang G, Cook AC, Postenka CO, Al-Katib W, Leong HS, Rodenhiser DI, Chambers AF and Tuck AB: Human 21T breast epithelial cell lines mimic breast cancer progression in vivo and in vitro and show stage-specific gene expression patterns. Lab Invest. 90:1247–1258. 2010. View Article : Google Scholar : PubMed/NCBI

30 

Aguirre Ghiso JA, Kovalski K and Ossowski L: Tumor dormancy induced by downregulation of urokinase receptor in human carcinoma involves integrin and MAPK signaling. J Cell Biol. 147:89–104. 1999. View Article : Google Scholar : PubMed/NCBI

31 

Aguirre Ghiso JA, Liu D, Mignatti A, Kovalski K and Ossowski L: Urokinase receptor and fibronectin regulate the ERKMAPK to p38MAPK activity ratios that determine carcinoma cell proliferation or dormancy in vivo. Mol Biol Cell. 12:863–879. 2001. View Article : Google Scholar : PubMed/NCBI

32 

Pavón MA, Arroyo-Solera I, Céspedes MV, Casanova I, León X and Mangues R: uPA/uPAR and SERPINE1 in head and neck cancer: Role in tumor resistance, metastasis, prognosis and therapy. Oncotarget. 7:57351–57366. 2016. View Article : Google Scholar : PubMed/NCBI

33 

Aguirre Ghiso JA: Inhibition of FAK signaling activated by urokinase receptor induces dormancy in human carcinoma cells in vivo. Oncogene. 21:2513–2524. 2002. View Article : Google Scholar : PubMed/NCBI

34 

Modi PK, Komaravelli N, Singh N and Sharma P: Interplay between MEK-ERK signaling, cyclin D1, and cyclin-dependent kinase 5 regulates cell cycle reentry and apoptosis of neurons. Mol Biol Cell. 23:3722–3730. 2012. View Article : Google Scholar : PubMed/NCBI

35 

Rössig L, Jadidi AS, Urbich C, Badorff C, Zeiher AM and Dimmeler S: Akt-dependent phosphorylation of p21Cip1 regulates PCNA binding and proliferation of endothelial cells. Mol Cell Biol. 21:5644–5657. 2001. View Article : Google Scholar : PubMed/NCBI

36 

Hodkinson PS, Mackinnon AC and Sethi T: Extracellular matrix regulation of drug resistance in small-cell lung cancer. Int J Radiat Biol. 83:733–741. 2007. View Article : Google Scholar : PubMed/NCBI

37 

Said G, Guilbert M, Morjani H, Garnotel R, Jeannesson P and El Btaouri H: Extracellular matrix proteins modulate antimigratory and apoptotic effects of Doxorubicin. Chemother Res Pract. 2012:2686812012.PubMed/NCBI

38 

Majidinia M and Yousefi B: Breast tumor stroma: A driving force in the development of resistance to therapies. Chem Biol Drug Des. 89:309–318. 2017. View Article : Google Scholar : PubMed/NCBI

39 

Fidler IJ, Wilmanns C, Staroselsky A, Radinsky R, Dong Z and Fan D: Modulation of tumor cell response to chemotherapy by the organ environment. Cancer Metastasis Rev. 13:209–222. 1994. View Article : Google Scholar : PubMed/NCBI

40 

Dalton WS: The tumor microenvironment as a determinant of drug response and resistance. Drug Resist Updat. 2:285–288. 1999. View Article : Google Scholar : PubMed/NCBI

41 

Buttery RC, Rintoul RC and Sethi T: Small cell lung cancer: The importance of the extracellular matrix. Int J Biochem Cell Biol. 36:1154–1160. 2004. View Article : Google Scholar : PubMed/NCBI

42 

Godwin P, Baird AM, Heavey S, Barr MP, O'Byrne KJ and Gately K: Targeting nuclear factor-kappa B to overcome resistance to chemotherapy. Front Oncol. 3:1202013. View Article : Google Scholar : PubMed/NCBI

43 

Ughachukwu P and Unekwe P: Efflux pump-mediated resistance in chemotherapy. Ann Med Health Sci Res. 2:191–198. 2012. View Article : Google Scholar : PubMed/NCBI

44 

Liu H, Patel MR, Prescher JA, Patsialou A, Qian D, Lin J, Wen S, Chang YF, Bachmann MH, Shimono Y, et al: Cancer stem cells from human breast tumors are involved in spontaneous metastases in orthotopic mouse models. Proc Natl Acad Sci USA. 107:pp. 18115–18120. 2010; View Article : Google Scholar : PubMed/NCBI

45 

Pajonk F, Vlashi E and McBride WH: Radiation resistance of cancer stem cells: The 4 R's of radiobiology revisited. Stem Cells. 28:639–648. 2010. View Article : Google Scholar : PubMed/NCBI

46 

D'Andrea FP: Intrinsic radiation resistance of mesenchymal cancer stem cells and implications for treatment response in a murine sarcoma model. Dan Med J. 59:B43882012.PubMed/NCBI

47 

LaBarge MA: The difficulty of targeting cancer stem cell niches. Clin Cancer Res. 16:3121–3129. 2010. View Article : Google Scholar : PubMed/NCBI

48 

Lacerda L, Pusztai L and Woodward WA: The role of tumor initiating cells in drug resistance of breast cancer: Implications for future therapeutic approaches. Drug Resist Updat. 13:99–108. 2010. View Article : Google Scholar : PubMed/NCBI

49 

Wang Z, Li Y, Ahmad A, Azmi AS, Kong D, Banerjee S and Sarkar FH: Targeting miRNAs involved in cancer stem cell and EMT regulation: An emerging concept in overcoming drug resistance. Drug Resist Updat. 13:109–118. 2010. View Article : Google Scholar : PubMed/NCBI

50 

Trumpp A, Essers M and Wilson A: Awakening dormant haematopoietic stem cells. Nat Rev Immunol. 10:201–209. 2010. View Article : Google Scholar : PubMed/NCBI

51 

Endo H, Okuyama H, Ohue M and Inoue M: Dormancy of cancer cells with suppression of AKT activity contributes to survival in chronic hypoxia. PLoS One. 9:e988582014. View Article : Google Scholar : PubMed/NCBI

52 

Li Q, Chow AB and Mattingly RR: Three-dimensional overlay culture models of human breast cancer reveal a critical sensitivity to mitogen-activated protein kinase kinase inhibitors. J Pharmacol Exp Ther. 332:821–828. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Keeratichamroen S, Lirdprapamongkol K and Svasti J: Mechanism of ECM-induced dormancy and chemoresistance in A549 human lung carcinoma cells. Oncol Rep 39: 1765-1774, 2018.
APA
Keeratichamroen, S., Lirdprapamongkol, K., & Svasti, J. (2018). Mechanism of ECM-induced dormancy and chemoresistance in A549 human lung carcinoma cells. Oncology Reports, 39, 1765-1774. https://doi.org/10.3892/or.2018.6258
MLA
Keeratichamroen, S., Lirdprapamongkol, K., Svasti, J."Mechanism of ECM-induced dormancy and chemoresistance in A549 human lung carcinoma cells". Oncology Reports 39.4 (2018): 1765-1774.
Chicago
Keeratichamroen, S., Lirdprapamongkol, K., Svasti, J."Mechanism of ECM-induced dormancy and chemoresistance in A549 human lung carcinoma cells". Oncology Reports 39, no. 4 (2018): 1765-1774. https://doi.org/10.3892/or.2018.6258
Copy and paste a formatted citation
x
Spandidos Publications style
Keeratichamroen S, Lirdprapamongkol K and Svasti J: Mechanism of ECM-induced dormancy and chemoresistance in A549 human lung carcinoma cells. Oncol Rep 39: 1765-1774, 2018.
APA
Keeratichamroen, S., Lirdprapamongkol, K., & Svasti, J. (2018). Mechanism of ECM-induced dormancy and chemoresistance in A549 human lung carcinoma cells. Oncology Reports, 39, 1765-1774. https://doi.org/10.3892/or.2018.6258
MLA
Keeratichamroen, S., Lirdprapamongkol, K., Svasti, J."Mechanism of ECM-induced dormancy and chemoresistance in A549 human lung carcinoma cells". Oncology Reports 39.4 (2018): 1765-1774.
Chicago
Keeratichamroen, S., Lirdprapamongkol, K., Svasti, J."Mechanism of ECM-induced dormancy and chemoresistance in A549 human lung carcinoma cells". Oncology Reports 39, no. 4 (2018): 1765-1774. https://doi.org/10.3892/or.2018.6258
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