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Article

Kaempferol inhibits angiogenic ability by targeting VEGF receptor-2 and downregulating the PI3K/AKT, MEK and ERK pathways in VEGF-stimulated human umbilical vein endothelial cells

  • Authors:
    • Hsien‑Kuo Chin
    • Chi‑Ting Horng
    • Yi‑Shan Liu
    • Chi‑Cheng Lu
    • Chen‑Ying Su
    • Pei‑Syuan Chen
    • Hong‑Yi Chiu
    • Fuu‑Jen Tsai
    • Po‑Chuen Shieh
    • Jai‑Sing Yang
  • View Affiliations / Copyright

    Affiliations: Department of Surgery, Kaohsiung Armed Forces General Hospital, Kaohsiung 80284, Taiwan, R.O.C., Medical Education Center, Kaohsiung Armed Forces General Hospital, Kaohsiung 80284, Taiwan, R.O.C., Department of Dermatology, E‑Da Hospital and I‑Shou University, Kaohsiung 82445, Taiwan, R.O.C., Department of Pharmacy, Buddhist Tzu Chi General Hospital, Hualien 97002, Taiwan, R.O.C., Department of Nursing, Tajen University, Pingtung 90741, Taiwan, R.O.C., Department of Pharmacy, Tajen University, Pingtung 90741, Taiwan, R.O.C., Huamn Genetics Center, Department of Medical Research, China Medical University Hospital, Taichung 40447, Taiwan, R.O.C., Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 40447, Taiwan, R.O.C.
  • Pages: 2351-2357
    |
    Published online on: March 14, 2018
       https://doi.org/10.3892/or.2018.6312
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Abstract

Anti-angiogenesis is one of the most general clinical obstacles in cancer chemotherapy. Kaempferol is a flavonoid phytochemical found in many fruits and vegetables. Our previous study revealed that kaempferol triggered apoptosis in human umbilical vein endothelial cells (HUVECs) by ROS‑mediated p53/ATM/death receptor signaling. However, the anti‑angiogenic potential of kaempferol remains unclear and its underlying mechanism warranted further exploration in VEGF‑stimulated HUVECs. In the present study, kaempferol significantly reduced VEGF‑stimulated HUVEC viability. Kaempferol treatment also inhibited cell migration, invasion, and tube formation in VEGF‑stimulated HUVECs. VEGF receptor‑2 (VEGFR‑2), and its downstream signaling cascades (such as AKT, mTOR and MEK1/2‑ERK1/2) were reduced as determined by western blotting and kinase activity assay in VEGF‑stimulated HUVECs after treatment with kaempferol. The present study revealed that kaempferol may possess angiogenic inhibition through regulation of VEGF/VEGFR‑2 and its downstream signaling cascades (PI3K/AKT, MEK and ERK) in VEGF-stimulated endothelial cells.
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Copy and paste a formatted citation
Spandidos Publications style
Chin HK, Horng CT, Liu YS, Lu CC, Su CY, Chen PS, Chiu HY, Tsai FJ, Shieh PC, Yang JS, Yang JS, et al: Kaempferol inhibits angiogenic ability by targeting VEGF receptor-2 and downregulating the PI3K/AKT, MEK and ERK pathways in VEGF-stimulated human umbilical vein endothelial cells. Oncol Rep 39: 2351-2357, 2018.
APA
Chin, H., Horng, C., Liu, Y., Lu, C., Su, C., Chen, P. ... Yang, J. (2018). Kaempferol inhibits angiogenic ability by targeting VEGF receptor-2 and downregulating the PI3K/AKT, MEK and ERK pathways in VEGF-stimulated human umbilical vein endothelial cells. Oncology Reports, 39, 2351-2357. https://doi.org/10.3892/or.2018.6312
MLA
Chin, H., Horng, C., Liu, Y., Lu, C., Su, C., Chen, P., Chiu, H., Tsai, F., Shieh, P., Yang, J."Kaempferol inhibits angiogenic ability by targeting VEGF receptor-2 and downregulating the PI3K/AKT, MEK and ERK pathways in VEGF-stimulated human umbilical vein endothelial cells". Oncology Reports 39.5 (2018): 2351-2357.
Chicago
Chin, H., Horng, C., Liu, Y., Lu, C., Su, C., Chen, P., Chiu, H., Tsai, F., Shieh, P., Yang, J."Kaempferol inhibits angiogenic ability by targeting VEGF receptor-2 and downregulating the PI3K/AKT, MEK and ERK pathways in VEGF-stimulated human umbilical vein endothelial cells". Oncology Reports 39, no. 5 (2018): 2351-2357. https://doi.org/10.3892/or.2018.6312
Copy and paste a formatted citation
x
Spandidos Publications style
Chin HK, Horng CT, Liu YS, Lu CC, Su CY, Chen PS, Chiu HY, Tsai FJ, Shieh PC, Yang JS, Yang JS, et al: Kaempferol inhibits angiogenic ability by targeting VEGF receptor-2 and downregulating the PI3K/AKT, MEK and ERK pathways in VEGF-stimulated human umbilical vein endothelial cells. Oncol Rep 39: 2351-2357, 2018.
APA
Chin, H., Horng, C., Liu, Y., Lu, C., Su, C., Chen, P. ... Yang, J. (2018). Kaempferol inhibits angiogenic ability by targeting VEGF receptor-2 and downregulating the PI3K/AKT, MEK and ERK pathways in VEGF-stimulated human umbilical vein endothelial cells. Oncology Reports, 39, 2351-2357. https://doi.org/10.3892/or.2018.6312
MLA
Chin, H., Horng, C., Liu, Y., Lu, C., Su, C., Chen, P., Chiu, H., Tsai, F., Shieh, P., Yang, J."Kaempferol inhibits angiogenic ability by targeting VEGF receptor-2 and downregulating the PI3K/AKT, MEK and ERK pathways in VEGF-stimulated human umbilical vein endothelial cells". Oncology Reports 39.5 (2018): 2351-2357.
Chicago
Chin, H., Horng, C., Liu, Y., Lu, C., Su, C., Chen, P., Chiu, H., Tsai, F., Shieh, P., Yang, J."Kaempferol inhibits angiogenic ability by targeting VEGF receptor-2 and downregulating the PI3K/AKT, MEK and ERK pathways in VEGF-stimulated human umbilical vein endothelial cells". Oncology Reports 39, no. 5 (2018): 2351-2357. https://doi.org/10.3892/or.2018.6312
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