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Article

CHAF1B knockdown blocks migration in a hepatocellular carcinoma model

  • Authors:
    • Xiaodong  Peng
    • Huiying  Fu
    • Jianjun  Yin
    • Qingmei  Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Department of Oncology, The 94th Hospital of The People's Liberation Army, Nanchang, Jiangxi 330000, P.R. China, Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Department of Oncology, Sichuan Mianyang 404 Hospital, Mianyang, Sichuan 361007, P.R. China
  • Pages: 405-413
    |
    Published online on: May 16, 2018
       https://doi.org/10.3892/or.2018.6437
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Abstract

The roles and model of action of the chromatin assembly complex factor-1B (CHAF1B) gene in liver cancer have not been fully elucidated. The CHAF1B gene in human hepatocellular carcinoma cell line HUH-7 was knocked down using a lentivirus and the transfected cells were assayed for migration and invasion abilities and cell cycle arrest using the scratch wound healingand Transwell assays as well as flow cytometry, respectively. Cells transfected with an empty vector were used as the control. The expression of genes was profiled. Models were constructed using CHAF1B-knockdown cells and investigated for tumor growth and pathological changes. Our experiments revealed that the knockdown of the CHAF1 gene reduced the invasion and migration ability of HUH-7 cells. Gene expression profiling revealed that after knockdown, PSMB6, SLC30A7, SMC3, TWF2 and BLM genes had the most marked changes as compared with the control. Western blot and RT-PCR analyses revealed that following the knockdown of the CHAF1B gene, protein and mRNA levels of the PSMB6, SLC30A7 and SMC3 genes were significantly upregulated, while those of the BLM and TWF2 genes were significantly downregulated. In the HUH-7-knockdown cells, there were significantly fewer G0/G1 cells and more S1 cells as compared with the control (36.10 vs. 54.10% and 59.7 vs. 40.8%, respectively), while the number of G2/M cells was similar (4.20 vs. 5.10%). The volumes of the tumors were similar between those injected with the empty vector and control, but were significantly smaller in the knockdown models, suggesting that the knockdown of the CHAF1B gene inhibited tumor growth. H&E staining revealed that tumors were developed in mice in all groups.
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1 

Niu J, Lin Y, Guo Z, Niu M and Su C: The epidemiological investigation on the risk factors of hepatocellular carcinoma: A case-control study in southeast china. Medicine. 95:e27582010. View Article : Google Scholar

2 

El-Serag HB and Mason AC: Rising incidence of hepatocellular carcinoma in the United States. N Engl J Med. 340:745–750. 1999. View Article : Google Scholar : PubMed/NCBI

3 

Llovet JM, Burroughs A and Bruix J: Hepatocellular carcinoma. Lancet. 362:1907–1917. 2003. View Article : Google Scholar : PubMed/NCBI

4 

Yang XD, Pan LH, Wang L, Ke Y, Cao J, Yang C, Zhong JH, Luo W, Guo J and Li LQ: Systematic review of single large and/or multinodular hepatocellular carcinoma: Surgical resection improves survival. Asian Pac J Cancer Prev. 16:5541–5547. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Lee CW, Tsai HI, Sung CM, Chen CW, Huang SW, Jeng WJ, Wu TH, Chan KM, Yu MC, Lee WC and Chen MF: Risk factors for early mortality after hepatectomy for hepatocellular carcinoma. Medicine. 95:e50282016. View Article : Google Scholar : PubMed/NCBI

6 

De Koning L, Corpet A, Haber JE and Almouzni G: Histone chaperones: An escort network regulating histone traffic. Nat Struct Mol Biol. 14:997–1007. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Xu M, Jia Y, Liu Z, Ding L, Tian R, Gu H, Wang Y, Zhang H, Tu K and Liu Q: Chromatin assembly factor 1, subunit A (P150) facilitates cell proliferation in human hepatocellular carcinoma. Onco Targets Ther. 9:4023–4035. 2016. View Article : Google Scholar : PubMed/NCBI

8 

Yu Z, Liu J, Deng WM and Jiao R: Histone chaperone CAF-1: Essential roles in multi-cellular organism development. Cell Mol Life Sci. 72:327–337. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Polo SE, Roche D and Almouzni G: New histone incorporation marks sites of UV repair in human cells. Cell. 127:481–493. 2006. View Article : Google Scholar : PubMed/NCBI

10 

Mascolo M, Ilardi G, Merolla F, Russo D, Vecchione ML, de Rosa G and Staibano S: Tissue microarray-based evaluation of chromatin assembly factor-1 (CAF-1)/p60 as tumour prognostic marker. Int J Mol Sci. 13:11044–11062. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Staibano S, Mascolo M, Rocco A, Lo Muzio L, Ilardi G, Siano M, Pannone G, Vecchione ML, Nugnes L, Califano L, et al: The proliferation marker chromatin assembly factor-1 is of clinical value in predicting the biological behaviour of salivary gland tumours. Oncol Rep. 25:13–22. 2011.PubMed/NCBI

12 

Jeffery DC, Kakusho N, You Z, Gharib M, Wyse B, Drury E, Weinreich M, Thibault P, Verreault A, Masai H and Yankulov K: CDC28 phosphorylates Cac1p and regulates the association of chromatin assembly factor I with chromatin. Cell Cycle. 14:74–85. 2015. View Article : Google Scholar : PubMed/NCBI

13 

Wu Z, Cui F, Yu F, Peng X, Jiang T, Chen D, Lu S, Tang H and Peng Z: Up-regulation of CHAF1A, a poor prognostic factor, facilitates cell proliferation of colon cancer. Biochem Biophys Res Commun. 449:208–215. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Mascolo M, Ayala F, Ilardi G, Balato A and Lembo S: Chromatin assembly factor-1/p60 overexpression: A potential index of psoriasis severity. Eur J Dermatol. 24:509–511. 2014.PubMed/NCBI

15 

Sarkar D, Leung EY, Baguley BC, Finlay GJ and Askarian-Amiri ME: Epigenetic regulation in human melanoma: Past and future. Epigenetics. 10:103–121. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Liang CC, Park AY and Guan JL: In vitro scratch assay: A convenient and inexpensive method for analysis of cell migration in vitro. Nat Protoc. 2:329–333. 2007. View Article : Google Scholar : PubMed/NCBI

17 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Volk A and Crispino JD: The role of the chromatin assembly complex (CAF-1) and its p60 subunit (CHAF1b) in homeostasis and disease. Biochim Biophys Acta. 1849:979–986. 2015. View Article : Google Scholar : PubMed/NCBI

19 

Verreault N, Da Costa D, Marchand A, Ireland K, Banack H, Dritsa M and Khalife S: PTSD following childbirth: Prospective study of incidence and risk factors incanadian women. J PsychosomRes. 73:257–266. 2012. View Article : Google Scholar

20 

Cheloufi S, Elling U, Hopfgartner B, Jung YL, Murn J, Ninova M, Hubmann M, Badeaux AI, Ang Euong C, Tenen D, et al: The histone chaperone CAF-1 safeguards somatic cell identity. Nature. 528:218–224. 2015. View Article : Google Scholar : PubMed/NCBI

21 

Kaufman PD, Kobayashi R, Kessler N and Stillman B: The p150 and p60 subunits of chromatin assembly factor I: A molecular link between newly synthesized histones and DNA replication. Cell. 81:1105–1114. 1995. View Article : Google Scholar : PubMed/NCBI

22 

Polo SE, Theocharis SE, Grandin L, Gambotti L, Antoni G, Savignoni A, Asselain B, Patsouris E and Almouzni G: Clinical significance and prognostic value of chromatin assembly factor-1 overexpression in human solid tumours. Histopathology. 57:716–724. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Mascolo M, Ilardi G, Romano MF, Celetti A, Siano M, Romano S, Luise C, Merolla F, Rocco A, Vecchione ML, et al: Overexpression of chromatin assembly factor-1 p60, poly(ADP-ribose) polymerase 1 and nestin predicts metastasizing behaviour of oral cancer. Histopathology. 61:1089–1105. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Kim D, Setiaputra D, Jung T, Chung J, Leitner A, Yoon J, Aebersold R, Hebert H, Yip CK and Song JJ: Molecular architecture of yeast chromatin assembly factor 1. Sci Rep. 6:267022016. View Article : Google Scholar : PubMed/NCBI

25 

Smith S and Stillman B: Purification and characterization of CAF-I, a human cell factor required for chromatin assembly during DNA replication in vitro. Cell. 58:15–25. 1989. View Article : Google Scholar : PubMed/NCBI

26 

Zhou H, Madden BJ, Muddiman DC and Zhang Z: Chromatin assembly factor 1 interacts with histone H3 methylated at lysine 79 in the processes of epigenetic silencing and DNA repair. Biochemistry. 45:2852–2861. 2006. View Article : Google Scholar : PubMed/NCBI

27 

Shibahara K and Stillman B: Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin. Cell. 96:575–585. 1999. View Article : Google Scholar : PubMed/NCBI

28 

Moggs JG, Grandi P, Quivy JP, Jónsson ZO, Hübscher U, Becker PB and Almouzni G: A CAF-1-PCNA-mediated chromatin assembly pathway triggered by sensing DNA damage. Mol Cell Biol. 20:1206–1218. 2000. View Article : Google Scholar : PubMed/NCBI

29 

Krude T: Chromatin assembly factor 1 (CAF-1) colocalizes with replication foci in HeLa cell nuclei. Exp Cell Res. 220:304–311. 1995. View Article : Google Scholar : PubMed/NCBI

30 

Li GM: New insights and challenges in mismatch repair: Getting over the chromatin hurdle. DNA Repair. 19:48–54. 2014. View Article : Google Scholar : PubMed/NCBI

31 

Adam S, Polo SE and Almouzni G: Transcription recovery after DNA damage requires chromatin priming by the H3.3 histone chaperone HIRA. Cell. 155:94–106. 2013. View Article : Google Scholar : PubMed/NCBI

32 

Baldeyron C, Soria G, Roche D, Cook AJ and Almouzni G: HP1alpha recruitment to DNA damage by p150CAF-1 promotes homologous recombination repair. J Cell Biol. 193:81–95. 2011. View Article : Google Scholar : PubMed/NCBI

33 

Yu Z, Wu H, Chen H, Wang R, Liang X, Liu J, Li C, Deng WM and Jiao R: CAF-1 promotes notch signaling through epigenetic control of target gene expression during drosophila development. Development. 140:3635–3644. 2013. View Article : Google Scholar : PubMed/NCBI

34 

Huang H, Yu Z, Zhang S, Liang X, Chen J, Li C, Ma J and Jiao R: Drosophila CAF-1 regulates HP1-mediated epigenetic silencing and pericentric heterochromatin stability. J Cell Sci. 123:2853–2861. 2010. View Article : Google Scholar : PubMed/NCBI

35 

Wen P, Quan Z and Xi R: The biological function of the WD40 repeat-containing protein p55/Caf1 in drosophila. Dev Dyn. 241:455–464. 2012. View Article : Google Scholar : PubMed/NCBI

36 

Staibano S, Mignogna C, Lo Muzio L, Mascolo M, Salvatore G, Di Benedetto M, Califano L, Rubini C and De Rosa G: Chromatin assembly factor-1 (CAF-1)-mediated regulation of cell proliferation and DNA repair: A link with the biological behaviour of squamous cell carcinoma of the tongue? Histopathology. 50:911–919. 2007. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Peng X, Fu H, Yin J and Zhao Q: CHAF1B knockdown blocks migration in a hepatocellular carcinoma model. Oncol Rep 40: 405-413, 2018.
APA
Peng, X., Fu, H., Yin, J., & Zhao, Q. (2018). CHAF1B knockdown blocks migration in a hepatocellular carcinoma model. Oncology Reports, 40, 405-413. https://doi.org/10.3892/or.2018.6437
MLA
Peng, X., Fu, H., Yin, J., Zhao, Q."CHAF1B knockdown blocks migration in a hepatocellular carcinoma model". Oncology Reports 40.1 (2018): 405-413.
Chicago
Peng, X., Fu, H., Yin, J., Zhao, Q."CHAF1B knockdown blocks migration in a hepatocellular carcinoma model". Oncology Reports 40, no. 1 (2018): 405-413. https://doi.org/10.3892/or.2018.6437
Copy and paste a formatted citation
x
Spandidos Publications style
Peng X, Fu H, Yin J and Zhao Q: CHAF1B knockdown blocks migration in a hepatocellular carcinoma model. Oncol Rep 40: 405-413, 2018.
APA
Peng, X., Fu, H., Yin, J., & Zhao, Q. (2018). CHAF1B knockdown blocks migration in a hepatocellular carcinoma model. Oncology Reports, 40, 405-413. https://doi.org/10.3892/or.2018.6437
MLA
Peng, X., Fu, H., Yin, J., Zhao, Q."CHAF1B knockdown blocks migration in a hepatocellular carcinoma model". Oncology Reports 40.1 (2018): 405-413.
Chicago
Peng, X., Fu, H., Yin, J., Zhao, Q."CHAF1B knockdown blocks migration in a hepatocellular carcinoma model". Oncology Reports 40, no. 1 (2018): 405-413. https://doi.org/10.3892/or.2018.6437
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