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Article

AAED1 modulates proliferation and glycolysis in gastric cancer

  • Authors:
    • Beili Zhang
    • Jugang Wu
    • Yantao Cai
    • Meng Luo
    • Bing Wang
    • Yan Gu
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China
  • Pages: 1156-1164
    |
    Published online on: June 7, 2018
       https://doi.org/10.3892/or.2018.6478
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Abstract

Gastric cancer is one of the most common and lethal malignancies globally, especially in East Asia. Although significant progress has been made in the diagnosis and treatment of the disease, the overall survival rate remains unchanged at 20‑25%. Thus, there is an urgent need for a better understanding of the disease. Recent years have witnessed the critical roles of aberrant cancer cell metabolism in the maintenance of malignant properties of cancer cells, and targeting cancer cell metabolism has been regarded as a novel aspect in the development of treatments against cancer. In the present study, we identified a novel gene, AAED1 (AhpC/TSA antioxidant enzyme domain containing 1), which is upregulated in gastric cancer cells. By using lentivirus mediated transfection method, we silenced AAED1 expression and silencing of AAED1 inhibited cancer cell proliferation in vitro in gastric cancer cell lines, as demonstrated by cell viability and colony formation assay. Furthermore, we uncovered novel functions of AAED1 in regulating hypoxia inducible factor 1α (HIF1α) and the resultant aerobic glycolysis, which was measured by extracellular flux analysis. Collectively, the results of the present study uncovered novel markers that could identify the possible molecular mechanisms involved in gastric cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang B, Wu J, Cai Y, Luo M, Wang B and Gu Y: AAED1 modulates proliferation and glycolysis in gastric cancer. Oncol Rep 40: 1156-1164, 2018.
APA
Zhang, B., Wu, J., Cai, Y., Luo, M., Wang, B., & Gu, Y. (2018). AAED1 modulates proliferation and glycolysis in gastric cancer. Oncology Reports, 40, 1156-1164. https://doi.org/10.3892/or.2018.6478
MLA
Zhang, B., Wu, J., Cai, Y., Luo, M., Wang, B., Gu, Y."AAED1 modulates proliferation and glycolysis in gastric cancer". Oncology Reports 40.2 (2018): 1156-1164.
Chicago
Zhang, B., Wu, J., Cai, Y., Luo, M., Wang, B., Gu, Y."AAED1 modulates proliferation and glycolysis in gastric cancer". Oncology Reports 40, no. 2 (2018): 1156-1164. https://doi.org/10.3892/or.2018.6478
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang B, Wu J, Cai Y, Luo M, Wang B and Gu Y: AAED1 modulates proliferation and glycolysis in gastric cancer. Oncol Rep 40: 1156-1164, 2018.
APA
Zhang, B., Wu, J., Cai, Y., Luo, M., Wang, B., & Gu, Y. (2018). AAED1 modulates proliferation and glycolysis in gastric cancer. Oncology Reports, 40, 1156-1164. https://doi.org/10.3892/or.2018.6478
MLA
Zhang, B., Wu, J., Cai, Y., Luo, M., Wang, B., Gu, Y."AAED1 modulates proliferation and glycolysis in gastric cancer". Oncology Reports 40.2 (2018): 1156-1164.
Chicago
Zhang, B., Wu, J., Cai, Y., Luo, M., Wang, B., Gu, Y."AAED1 modulates proliferation and glycolysis in gastric cancer". Oncology Reports 40, no. 2 (2018): 1156-1164. https://doi.org/10.3892/or.2018.6478
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