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TMEM165, a Golgi transmembrane protein, is a novel marker for hepatocellular carcinoma and its depletion impairs invasion activity

  • Authors:
    • Jee‑San Lee
    • Mi‑Yeun Kim
    • Eun‑Ran Park
    • Yan Nan Shen
    • Ju‑Yeon Jeon
    • Eung‑Ho Cho
    • Sun‑Hoo Park
    • Chul Ju Han
    • Dong Wook Choi
    • Ja June Jang
    • Kyung‑Suk Suh
    • Jungil Hong
    • Sang Bum Kim
    • Kee‑Ho Lee
  • View Affiliations / Copyright

    Affiliations: Division of Radiation Cancer Research, Korea Institute of Radiological and Medical Sciences, Seoul 01812, Republic of Korea, Department of Surgery, Korea Institute of Radiological and Medical Sciences, Seoul 01812, Republic of Korea, Department of Pathology, Korea Institute of Radiological and Medical Sciences, Seoul 01812, Republic of Korea, Department of Internal Medicine, Korea Institute of Radiological and Medical Sciences, Seoul 01812, Republic of Korea, Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, Republic of Korea, Department of Pathology, Seoul National University School of Medicine, Seoul 08226, Republic of Korea, Department of Surgery, Seoul National University School of Medicine, Seoul 08226, Republic of Korea, Division of Applied Food System, College of Natural Science, Seoul Women's University, Seoul 01797, Republic of Korea
    Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1297-1306
    |
    Published online on: July 12, 2018
       https://doi.org/10.3892/or.2018.6565
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Abstract

Transmembrane protein 165 (TMEM165), a Golgi protein, functions in ion homeostasis and vesicular trafficking in the Golgi apparatus. While mutations in TMEM165 are known to cause human ‘congenital disorders of glycosylation’, a recessive autosomal metabolic disease, the potential association of this protein with human cancer development has not been explored to date. In the present study, we revealed that TMEM165 is overexpressed in HCC and its depletion weakens the invasive activity of cancer cells through suppression of matrix metalloproteinase‑2 (MMP‑2) expression. Levels of TMEM165 mRNA and protein were clearly increased in HCC patient tissues and cell cultures. Quantitative real‑time RT‑PCR analysis of fresh HCC tissues (n=88) revealed association of TMEM165 overexpression with more frequent macroscopic vascular invasion, microscopic serosal invasion and higher α‑fetoprotein levels. Notably, depletion of TMEM165 led to a marked decrease in the invasive activity of two different HCC cell types, Huh7 and SNU475, accompanied by downregulation of MMP‑2. Our collective findings clearly indicated that TMEM165 contributed to the progression of HCC by promoting invasive activity, supporting its utility as a novel biomarker and therapeutic target for cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Lee JS, Kim MY, Park ER, Shen YN, Jeon JY, Cho EH, Park SH, Han CJ, Choi DW, Jang JJ, Jang JJ, et al: TMEM165, a Golgi transmembrane protein, is a novel marker for hepatocellular carcinoma and its depletion impairs invasion activity. Oncol Rep 40: 1297-1306, 2018.
APA
Lee, J., Kim, M., Park, E., Shen, Y.N., Jeon, J., Cho, E. ... Lee, K. (2018). TMEM165, a Golgi transmembrane protein, is a novel marker for hepatocellular carcinoma and its depletion impairs invasion activity. Oncology Reports, 40, 1297-1306. https://doi.org/10.3892/or.2018.6565
MLA
Lee, J., Kim, M., Park, E., Shen, Y. N., Jeon, J., Cho, E., Park, S., Han, C. J., Choi, D. W., Jang, J. J., Suh, K., Hong, J., Kim, S. B., Lee, K."TMEM165, a Golgi transmembrane protein, is a novel marker for hepatocellular carcinoma and its depletion impairs invasion activity". Oncology Reports 40.3 (2018): 1297-1306.
Chicago
Lee, J., Kim, M., Park, E., Shen, Y. N., Jeon, J., Cho, E., Park, S., Han, C. J., Choi, D. W., Jang, J. J., Suh, K., Hong, J., Kim, S. B., Lee, K."TMEM165, a Golgi transmembrane protein, is a novel marker for hepatocellular carcinoma and its depletion impairs invasion activity". Oncology Reports 40, no. 3 (2018): 1297-1306. https://doi.org/10.3892/or.2018.6565
Copy and paste a formatted citation
x
Spandidos Publications style
Lee JS, Kim MY, Park ER, Shen YN, Jeon JY, Cho EH, Park SH, Han CJ, Choi DW, Jang JJ, Jang JJ, et al: TMEM165, a Golgi transmembrane protein, is a novel marker for hepatocellular carcinoma and its depletion impairs invasion activity. Oncol Rep 40: 1297-1306, 2018.
APA
Lee, J., Kim, M., Park, E., Shen, Y.N., Jeon, J., Cho, E. ... Lee, K. (2018). TMEM165, a Golgi transmembrane protein, is a novel marker for hepatocellular carcinoma and its depletion impairs invasion activity. Oncology Reports, 40, 1297-1306. https://doi.org/10.3892/or.2018.6565
MLA
Lee, J., Kim, M., Park, E., Shen, Y. N., Jeon, J., Cho, E., Park, S., Han, C. J., Choi, D. W., Jang, J. J., Suh, K., Hong, J., Kim, S. B., Lee, K."TMEM165, a Golgi transmembrane protein, is a novel marker for hepatocellular carcinoma and its depletion impairs invasion activity". Oncology Reports 40.3 (2018): 1297-1306.
Chicago
Lee, J., Kim, M., Park, E., Shen, Y. N., Jeon, J., Cho, E., Park, S., Han, C. J., Choi, D. W., Jang, J. J., Suh, K., Hong, J., Kim, S. B., Lee, K."TMEM165, a Golgi transmembrane protein, is a novel marker for hepatocellular carcinoma and its depletion impairs invasion activity". Oncology Reports 40, no. 3 (2018): 1297-1306. https://doi.org/10.3892/or.2018.6565
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