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Article

Staurosporine alleviates cisplatin chemoresistance in human cancer cell models by suppressing the induction of SQSTM1/p62

  • Authors:
    • Khaldoon Alsamman
    • Omar S. El‑Masry
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Imam Abdulrahman Bin Faisal University, Dammam 31441, Kingdom of Saudi Arabia, Department of Applied Medical Chemistry, Medical Research Institute, University of Alexandria, Alexandria 21561, Egypt
  • Pages: 2157-2162
    |
    Published online on: August 1, 2018
       https://doi.org/10.3892/or.2018.6615
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Abstract

Cancer is one of the leading causes of mortality worldwide. Platinum‑based chemotherapeutic agents such as cisplatin are the first line of treatment for many types of cancers. However, the development of cisplatin resistance after prolonged treatment is a common cause of cancer recurrence. In the present study, we investigated an approach designed to overcome resistance to cisplatin involving co‑treatment with a second chemotherapeutic agent, staurosporine, and examined the role of sequestosome 1 (SQSTM1/p62) in enhancing cellular sensitivity to cisplatin. We utilized experimental models of three different cancers comprising cell lines derived from colon, breast, and ovarian tumors and investigated cell proliferation, morphology and p62 levels after treatment with cisplatin, staurosporine, or a combination of the two. Western blot analysis showed that cisplatin treatment resulted in elevation of p62 levels when compared to the corresponding control cells. Conversely, treatment with staurosporine resulted in a marked reduction in p62 levels in all three cell types and abrogated the cisplatin‑induced upregulation of p62. These results suggest that staurosporine could sensitize cancer cells to cisplatin via a mechanism involving downregulation of p62.
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Copy and paste a formatted citation
Spandidos Publications style
Alsamman K and El‑Masry OS: Staurosporine alleviates cisplatin chemoresistance in human cancer cell models by suppressing the induction of SQSTM1/p62. Oncol Rep 40: 2157-2162, 2018.
APA
Alsamman, K., & El‑Masry, O.S. (2018). Staurosporine alleviates cisplatin chemoresistance in human cancer cell models by suppressing the induction of SQSTM1/p62. Oncology Reports, 40, 2157-2162. https://doi.org/10.3892/or.2018.6615
MLA
Alsamman, K., El‑Masry, O. S."Staurosporine alleviates cisplatin chemoresistance in human cancer cell models by suppressing the induction of SQSTM1/p62". Oncology Reports 40.4 (2018): 2157-2162.
Chicago
Alsamman, K., El‑Masry, O. S."Staurosporine alleviates cisplatin chemoresistance in human cancer cell models by suppressing the induction of SQSTM1/p62". Oncology Reports 40, no. 4 (2018): 2157-2162. https://doi.org/10.3892/or.2018.6615
Copy and paste a formatted citation
x
Spandidos Publications style
Alsamman K and El‑Masry OS: Staurosporine alleviates cisplatin chemoresistance in human cancer cell models by suppressing the induction of SQSTM1/p62. Oncol Rep 40: 2157-2162, 2018.
APA
Alsamman, K., & El‑Masry, O.S. (2018). Staurosporine alleviates cisplatin chemoresistance in human cancer cell models by suppressing the induction of SQSTM1/p62. Oncology Reports, 40, 2157-2162. https://doi.org/10.3892/or.2018.6615
MLA
Alsamman, K., El‑Masry, O. S."Staurosporine alleviates cisplatin chemoresistance in human cancer cell models by suppressing the induction of SQSTM1/p62". Oncology Reports 40.4 (2018): 2157-2162.
Chicago
Alsamman, K., El‑Masry, O. S."Staurosporine alleviates cisplatin chemoresistance in human cancer cell models by suppressing the induction of SQSTM1/p62". Oncology Reports 40, no. 4 (2018): 2157-2162. https://doi.org/10.3892/or.2018.6615
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