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MicroRNA‑181a promotes cell proliferation and inhibits apoptosis in gastric cancer by targeting RASSF1A

  • Authors:
    • Junhui Yu
    • Jie Qi
    • Xuejun Sun
    • Wei Wang
    • Guangbing Wei
    • Yunhua Wu
    • Qi Gao
    • Jianbao Zheng
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, Second Department of Cardiovascular Medicine, Shaanxi Provincial People's Hospital, Xi'an, Shaanxi 710068, P.R. China, Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China
    Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1959-1970
    |
    Published online on: August 7, 2018
       https://doi.org/10.3892/or.2018.6632
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Abstract

MicroRNA (miR)‑181a is a member of the miR‑181 family that serves a key role in the pathogenesis of various cancer types. The present study aimed to investigate the interaction between miR‑181a and Ras association domain family protein1 isoform A (RASSF1A), and their roles in gastric carcinogenesis. The interaction between miR‑181a and RASSF1A was assessed in cell lines and cancer tissues. The direct binding of miR‑181a and RASSF1A was identified using a luciferase reporting gene system. The effects of miR‑181a and RASSF1A on gastric cancer cell growth, cell cycle and apoptosis were assessed with a Cell Counting Kit‑8 assay and flow cytometry. The effects of miR‑181a on cell division cycle 25A (CDC25A), cyclin A2, cyclin D1, p21, Bcl‑2‑associated X protein (Bax) and B‑cell lymphoma‑2 (Bcl‑2) protein levels were assessed in gastric cancer cell lines. miR‑181a directly interacted with the 3'‑untranslated region of RASSF1A and downregulated RASSF1A protein expression. In tissues from patients with gastric cancer, the miR‑181a level was significantly higher in the tumor tissues and was negatively correlated with the RASSF1A protein level. RASSF1A suppressed gastric cancer cell proliferation and G1/S transition, and promoted apoptosis; whereas miR‑181a promoted cancer cell proliferation and G1/S transition, and suppressed apoptosis. RASSF1A knockdown attenuated the effects of miR‑181a downregulation on cell proliferation and apoptosis. Furthermore, miR‑181a upregulated CDC25A, cyclin A2 and Bcl‑2, and downregulated Bax protein expression in gastric cancer cell lines. These data indicate that miR‑181a promotes gastric carcinogenesis, possibly through a direct interaction with RASSF1A.
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Copy and paste a formatted citation
Spandidos Publications style
Yu J, Qi J, Sun X, Wang W, Wei G, Wu Y, Gao Q and Zheng J: MicroRNA‑181a promotes cell proliferation and inhibits apoptosis in gastric cancer by targeting RASSF1A. Oncol Rep 40: 1959-1970, 2018.
APA
Yu, J., Qi, J., Sun, X., Wang, W., Wei, G., Wu, Y. ... Zheng, J. (2018). MicroRNA‑181a promotes cell proliferation and inhibits apoptosis in gastric cancer by targeting RASSF1A. Oncology Reports, 40, 1959-1970. https://doi.org/10.3892/or.2018.6632
MLA
Yu, J., Qi, J., Sun, X., Wang, W., Wei, G., Wu, Y., Gao, Q., Zheng, J."MicroRNA‑181a promotes cell proliferation and inhibits apoptosis in gastric cancer by targeting RASSF1A". Oncology Reports 40.4 (2018): 1959-1970.
Chicago
Yu, J., Qi, J., Sun, X., Wang, W., Wei, G., Wu, Y., Gao, Q., Zheng, J."MicroRNA‑181a promotes cell proliferation and inhibits apoptosis in gastric cancer by targeting RASSF1A". Oncology Reports 40, no. 4 (2018): 1959-1970. https://doi.org/10.3892/or.2018.6632
Copy and paste a formatted citation
x
Spandidos Publications style
Yu J, Qi J, Sun X, Wang W, Wei G, Wu Y, Gao Q and Zheng J: MicroRNA‑181a promotes cell proliferation and inhibits apoptosis in gastric cancer by targeting RASSF1A. Oncol Rep 40: 1959-1970, 2018.
APA
Yu, J., Qi, J., Sun, X., Wang, W., Wei, G., Wu, Y. ... Zheng, J. (2018). MicroRNA‑181a promotes cell proliferation and inhibits apoptosis in gastric cancer by targeting RASSF1A. Oncology Reports, 40, 1959-1970. https://doi.org/10.3892/or.2018.6632
MLA
Yu, J., Qi, J., Sun, X., Wang, W., Wei, G., Wu, Y., Gao, Q., Zheng, J."MicroRNA‑181a promotes cell proliferation and inhibits apoptosis in gastric cancer by targeting RASSF1A". Oncology Reports 40.4 (2018): 1959-1970.
Chicago
Yu, J., Qi, J., Sun, X., Wang, W., Wei, G., Wu, Y., Gao, Q., Zheng, J."MicroRNA‑181a promotes cell proliferation and inhibits apoptosis in gastric cancer by targeting RASSF1A". Oncology Reports 40, no. 4 (2018): 1959-1970. https://doi.org/10.3892/or.2018.6632
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