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miR‑590‑5p inhibits tumor growth in malignant melanoma by suppressing YAP1 expression

  • Authors:
    • Kuanhou Mou
    • Meiling Ding
    • Dan Han
    • Yan Zhou
    • Xin Mu
    • Wenli Liu
    • Lijuan Wang
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, State Key Laboratory of Cancer Biology, National Clinical Research Center for Digestive Disease, Xijing Hospital of Digestive Diseases, The Fourth Military Medical University, Xi'an, Shaanxi 710069, P.R. China, Department of Dermatology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China
    Copyright: © Mou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2056-2066
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    Published online on: August 7, 2018
       https://doi.org/10.3892/or.2018.6633
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Abstract

The microRNAs (miRNAs/miRs) involved in the carcinogenesis and progression of malignant melanoma (MM) remain unclear. In the present study, miR‑590‑5p was identified to be upregulated in MM cells compared with human melanocytes using a reverse transcription‑quantitative polymerase chain reaction to screen established oncogenic and tumor suppressor miRNAs. miR‑590‑5p was demonstrated to inhibit the cell proliferation and tumor growth of MM cells in vitro and in vivo by performing Cell Counting Kit‑8 and tumour xenograft assays, respectively. In addition, flowcytometry assays indicated that miR‑590‑5p induced cell apoptosis and cell cycle arrest at the G1 stage in MM cells. Finally, luciferase assays and western blot analysis results confirmed that the transcriptional regulator Yes‑associated protein 1 (YAP1) is upregulated and inversely associated with miR‑590‑5p expression in MM cells, and is the direct target and functional mediator of miR‑590‑5p in MM. Altogether these results reveal the functional and mechanistic link between miR‑590‑5p and YAP1 in the progression of MM. Therefore, miR‑590‑5p is a potential therapeutic target in MM.
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Copy and paste a formatted citation
Spandidos Publications style
Mou K, Ding M, Han D, Zhou Y, Mu X, Liu W and Wang L: miR‑590‑5p inhibits tumor growth in malignant melanoma by suppressing YAP1 expression. Oncol Rep 40: 2056-2066, 2018.
APA
Mou, K., Ding, M., Han, D., Zhou, Y., Mu, X., Liu, W., & Wang, L. (2018). miR‑590‑5p inhibits tumor growth in malignant melanoma by suppressing YAP1 expression. Oncology Reports, 40, 2056-2066. https://doi.org/10.3892/or.2018.6633
MLA
Mou, K., Ding, M., Han, D., Zhou, Y., Mu, X., Liu, W., Wang, L."miR‑590‑5p inhibits tumor growth in malignant melanoma by suppressing YAP1 expression". Oncology Reports 40.4 (2018): 2056-2066.
Chicago
Mou, K., Ding, M., Han, D., Zhou, Y., Mu, X., Liu, W., Wang, L."miR‑590‑5p inhibits tumor growth in malignant melanoma by suppressing YAP1 expression". Oncology Reports 40, no. 4 (2018): 2056-2066. https://doi.org/10.3892/or.2018.6633
Copy and paste a formatted citation
x
Spandidos Publications style
Mou K, Ding M, Han D, Zhou Y, Mu X, Liu W and Wang L: miR‑590‑5p inhibits tumor growth in malignant melanoma by suppressing YAP1 expression. Oncol Rep 40: 2056-2066, 2018.
APA
Mou, K., Ding, M., Han, D., Zhou, Y., Mu, X., Liu, W., & Wang, L. (2018). miR‑590‑5p inhibits tumor growth in malignant melanoma by suppressing YAP1 expression. Oncology Reports, 40, 2056-2066. https://doi.org/10.3892/or.2018.6633
MLA
Mou, K., Ding, M., Han, D., Zhou, Y., Mu, X., Liu, W., Wang, L."miR‑590‑5p inhibits tumor growth in malignant melanoma by suppressing YAP1 expression". Oncology Reports 40.4 (2018): 2056-2066.
Chicago
Mou, K., Ding, M., Han, D., Zhou, Y., Mu, X., Liu, W., Wang, L."miR‑590‑5p inhibits tumor growth in malignant melanoma by suppressing YAP1 expression". Oncology Reports 40, no. 4 (2018): 2056-2066. https://doi.org/10.3892/or.2018.6633
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