Open Access

miR‑590‑5p inhibits tumor growth in malignant melanoma by suppressing YAP1 expression

  • Authors:
    • Kuanhou Mou
    • Meiling Ding
    • Dan Han
    • Yan Zhou
    • Xin Mu
    • Wenli Liu
    • Lijuan Wang
  • View Affiliations

  • Published online on: August 7, 2018     https://doi.org/10.3892/or.2018.6633
  • Pages: 2056-2066
  • Copyright: © Mou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The microRNAs (miRNAs/miRs) involved in the carcinogenesis and progression of malignant melanoma (MM) remain unclear. In the present study, miR‑590‑5p was identified to be upregulated in MM cells compared with human melanocytes using a reverse transcription‑quantitative polymerase chain reaction to screen established oncogenic and tumor suppressor miRNAs. miR‑590‑5p was demonstrated to inhibit the cell proliferation and tumor growth of MM cells in vitro and in vivo by performing Cell Counting Kit‑8 and tumour xenograft assays, respectively. In addition, flowcytometry assays indicated that miR‑590‑5p induced cell apoptosis and cell cycle arrest at the G1 stage in MM cells. Finally, luciferase assays and western blot analysis results confirmed that the transcriptional regulator Yes‑associated protein 1 (YAP1) is upregulated and inversely associated with miR‑590‑5p expression in MM cells, and is the direct target and functional mediator of miR‑590‑5p in MM. Altogether these results reveal the functional and mechanistic link between miR‑590‑5p and YAP1 in the progression of MM. Therefore, miR‑590‑5p is a potential therapeutic target in MM.
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October-2018
Volume 40 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Mou K, Ding M, Han D, Zhou Y, Mu X, Liu W and Wang L: miR‑590‑5p inhibits tumor growth in malignant melanoma by suppressing YAP1 expression. Oncol Rep 40: 2056-2066, 2018
APA
Mou, K., Ding, M., Han, D., Zhou, Y., Mu, X., Liu, W., & Wang, L. (2018). miR‑590‑5p inhibits tumor growth in malignant melanoma by suppressing YAP1 expression. Oncology Reports, 40, 2056-2066. https://doi.org/10.3892/or.2018.6633
MLA
Mou, K., Ding, M., Han, D., Zhou, Y., Mu, X., Liu, W., Wang, L."miR‑590‑5p inhibits tumor growth in malignant melanoma by suppressing YAP1 expression". Oncology Reports 40.4 (2018): 2056-2066.
Chicago
Mou, K., Ding, M., Han, D., Zhou, Y., Mu, X., Liu, W., Wang, L."miR‑590‑5p inhibits tumor growth in malignant melanoma by suppressing YAP1 expression". Oncology Reports 40, no. 4 (2018): 2056-2066. https://doi.org/10.3892/or.2018.6633