A novel peptide targets CD105 for tumour imaging in vivo

  • Authors:
    • Xiaolong Li
    • Xiaofeng Huang
    • Jing Zhang
    • Hongxing Huang
    • Long Zhao
    • Mei Yu
    • Yan Zhang
    • Hua Wang
  • View Affiliations

  • Published online on: August 14, 2018     https://doi.org/10.3892/or.2018.6643
  • Pages: 2935-2943
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Abstract

Non‑antibody‑binding proteins (nABPs) have attracted attention in the field of alternative antibody approaches due to their ability to penetrate cell membranes. CD105 has been demonstrated to be a useful marker for identifying angiogenic endothelial, mesenchymal stem and osteosarcoma cells. In the present study, a novel non‑antibody‑binding protein (namely nABP296) with a high affinity for recombinant human CD105 was identified by M13 phage biopanning. Fluorescence microscopy, flow cytometry and in vivo animal imaging were used to confirm the visualization effect of the novel peptide to the CD105‑positive MNNG/HOS cell line in vivo and in vitro. Immunofluorescence of tissue sections was also used to identify the target efficiency of the peptide in tumour sections derived from an MNNG/HOS xenograft tumour model and osteosarcoma patients. This peptide specifically was found to bind to the CD105‑positive osteosarcoma MNNG/HOS cell line and the osteosarcoma cells in the histological sections derived from an MNNG/HOS xenograft model and osteosarcoma patients in vitro. This peptide also successfully labelled an animal osteosarcoma xenograft model in vivo. Taken together, a novel peptide that can be used as a potent agent for CD105 molecular targeting was identified, and this peptide can be applied for osteosarcoma visualization in vitro and in vivo.
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November-2018
Volume 40 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Li X, Huang X, Zhang J, Huang H, Zhao L, Yu M, Zhang Y and Wang H: A novel peptide targets CD105 for tumour imaging in vivo. Oncol Rep 40: 2935-2943, 2018
APA
Li, X., Huang, X., Zhang, J., Huang, H., Zhao, L., Yu, M. ... Wang, H. (2018). A novel peptide targets CD105 for tumour imaging in vivo. Oncology Reports, 40, 2935-2943. https://doi.org/10.3892/or.2018.6643
MLA
Li, X., Huang, X., Zhang, J., Huang, H., Zhao, L., Yu, M., Zhang, Y., Wang, H."A novel peptide targets CD105 for tumour imaging in vivo". Oncology Reports 40.5 (2018): 2935-2943.
Chicago
Li, X., Huang, X., Zhang, J., Huang, H., Zhao, L., Yu, M., Zhang, Y., Wang, H."A novel peptide targets CD105 for tumour imaging in vivo". Oncology Reports 40, no. 5 (2018): 2935-2943. https://doi.org/10.3892/or.2018.6643