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Article

Altered mRNA expression levels of the major components of sphingolipid metabolism, ceramide synthases and their clinical implication in colorectal cancer

  • Authors:
    • Sung Won Jang
    • Woo‑Jae Park
    • Hyeonji Min
    • Taeg Kyu Kwon
    • Seong Kyu Baek
    • Ilseon Hwang
    • Shin Kim
    • Jong‑Wook Park
  • View Affiliations / Copyright

    Affiliations: Department of Emergency Medicine, Dongsan Medical Center, Keimyung University, Daegu 41931, Republic of Korea, Department of Biochemistry, College of Medicine, Gachon University, Incheon 21999, Republic of Korea, Department of Immunology, School of Medicine, Keimyung University, Daegu 42601, P.R. China, Department of Immunology, School of Medicine, Keimyung University, Daegu 42601, P.R. China, Department of Surgery, Dongsan Medical Center, Keimyung University, Daegu 41931, Republic of Korea, Department of Pathology, School of Medicine, Keimyung University, Daegu 42601, Republic of Korea
  • Pages: 3489-3500
    |
    Published online on: September 18, 2018
       https://doi.org/10.3892/or.2018.6712
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Abstract

Ceramide synthases (CerSs) synthesize various ceramides of different acyl chain lengths and serve important roles in the proliferation and death of cancer cells by regulating sphingolipid metabolism‑related signaling pathways. The present study investigated the mRNA expression levels of various CerS genes using mRNA expression data from six independent colorectal cancer (CRC) cohorts and a Korean CRC dataset. Expression levels of CERS2, CERS5 and CERS6 mRNA were significantly increased in the majority of the studied groups. However, CERS4 expression was only significantly altered in two groups. Additionally, a positive correlation was observed between altered CERS4 and CERS5 mRNA levels in The Cancer Genome Atlas Colon and Rectal Cancer dataset. Notably, CERS2 and CERS4, as well as CERS5 and CERS6 levels, were positively correlated with each other in Korean patients with CRC. However, the mRNA expression levels of these four CerS genes were not associated with any clinicopathological characteristics in Korean patients with CRC. Finally, overexpressing CERS2 or CERS6 inhibited the in vitro viability of various CRC cells. Taken together, these findings indicated that CERS2, CERS4, CERS5, and CERS6 are significantly dysregulated in CRC, suggesting they may serve important roles in the pathophysiology of this malignancy.
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Copy and paste a formatted citation
Spandidos Publications style
Jang SW, Park WJ, Min H, Kwon TK, Baek SK, Hwang I, Kim S and Park JW: Altered mRNA expression levels of the major components of sphingolipid metabolism, ceramide synthases and their clinical implication in colorectal cancer. Oncol Rep 40: 3489-3500, 2018.
APA
Jang, S.W., Park, W., Min, H., Kwon, T.K., Baek, S.K., Hwang, I. ... Park, J. (2018). Altered mRNA expression levels of the major components of sphingolipid metabolism, ceramide synthases and their clinical implication in colorectal cancer. Oncology Reports, 40, 3489-3500. https://doi.org/10.3892/or.2018.6712
MLA
Jang, S. W., Park, W., Min, H., Kwon, T. K., Baek, S. K., Hwang, I., Kim, S., Park, J."Altered mRNA expression levels of the major components of sphingolipid metabolism, ceramide synthases and their clinical implication in colorectal cancer". Oncology Reports 40.6 (2018): 3489-3500.
Chicago
Jang, S. W., Park, W., Min, H., Kwon, T. K., Baek, S. K., Hwang, I., Kim, S., Park, J."Altered mRNA expression levels of the major components of sphingolipid metabolism, ceramide synthases and their clinical implication in colorectal cancer". Oncology Reports 40, no. 6 (2018): 3489-3500. https://doi.org/10.3892/or.2018.6712
Copy and paste a formatted citation
x
Spandidos Publications style
Jang SW, Park WJ, Min H, Kwon TK, Baek SK, Hwang I, Kim S and Park JW: Altered mRNA expression levels of the major components of sphingolipid metabolism, ceramide synthases and their clinical implication in colorectal cancer. Oncol Rep 40: 3489-3500, 2018.
APA
Jang, S.W., Park, W., Min, H., Kwon, T.K., Baek, S.K., Hwang, I. ... Park, J. (2018). Altered mRNA expression levels of the major components of sphingolipid metabolism, ceramide synthases and their clinical implication in colorectal cancer. Oncology Reports, 40, 3489-3500. https://doi.org/10.3892/or.2018.6712
MLA
Jang, S. W., Park, W., Min, H., Kwon, T. K., Baek, S. K., Hwang, I., Kim, S., Park, J."Altered mRNA expression levels of the major components of sphingolipid metabolism, ceramide synthases and their clinical implication in colorectal cancer". Oncology Reports 40.6 (2018): 3489-3500.
Chicago
Jang, S. W., Park, W., Min, H., Kwon, T. K., Baek, S. K., Hwang, I., Kim, S., Park, J."Altered mRNA expression levels of the major components of sphingolipid metabolism, ceramide synthases and their clinical implication in colorectal cancer". Oncology Reports 40, no. 6 (2018): 3489-3500. https://doi.org/10.3892/or.2018.6712
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