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Cellular communication promotes mammosphere growth and collective invasion through microtubule‑like structures and angiogenesis

  • Authors:
    • Shangke Huang
    • Na Yuan
    • Guanying Wang
    • Fang Wu
    • Lu Feng
    • Minna Luo
    • Miao Li
    • Anqi Luo
    • Xinhan Zhao
    • Lingxiao Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Medical Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, Department of Ultrasound, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, Department of Neonatology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, Department of Hematology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, Department of Medical Oncology, The Fifth People's Hospital of Qinghai Province, Xining, Qinghai 810007, P.R. China
    Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3297-3312
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    Published online on: October 9, 2018
       https://doi.org/10.3892/or.2018.6778
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Abstract

Networks of nanotubes and microtubules are highly valued in cellular communication, and collective cancer movement has been revealed to be associated with cell information exchange. In the present study, cellular communication was demonstrated to participate in mammosphere growth, differentiation and collective invasion. By promoting differentiation, networks of cells and microtubule‑like structures were verified. Analyses of cell cycle progression, stemness markers and gene expression indicated that mammospheres had collective characteristics of stemness and differentiation. Invasion assays revealed that networks of microtubule‑like structures promoted collective invasion. Conversely, using anti‑angiogenic intervention, the growth of stem‑like mammospheres and cellular communication links were effectively inhibited. In vivo experiments revealed that cellular communication promoted tumor growth and metastasis through the formation of nodular fusion, cluttered microtubule‑like structures and cancer stem cells, as well as vascular niches. In conclusion, the present results demonstrated that a network of cells and structures were largely present in mammosphere cellular communication in vitro and in vivo. Therefore, blocking cellular communication may prove beneficial in halting the progression of mammary tumors.
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Copy and paste a formatted citation
Spandidos Publications style
Huang S, Yuan N, Wang G, Wu F, Feng L, Luo M, Li M, Luo A, Zhao X, Zhang L, Zhang L, et al: Cellular communication promotes mammosphere growth and collective invasion through microtubule‑like structures and angiogenesis. Oncol Rep 40: 3297-3312, 2018.
APA
Huang, S., Yuan, N., Wang, G., Wu, F., Feng, L., Luo, M. ... Zhang, L. (2018). Cellular communication promotes mammosphere growth and collective invasion through microtubule‑like structures and angiogenesis. Oncology Reports, 40, 3297-3312. https://doi.org/10.3892/or.2018.6778
MLA
Huang, S., Yuan, N., Wang, G., Wu, F., Feng, L., Luo, M., Li, M., Luo, A., Zhao, X., Zhang, L."Cellular communication promotes mammosphere growth and collective invasion through microtubule‑like structures and angiogenesis". Oncology Reports 40.6 (2018): 3297-3312.
Chicago
Huang, S., Yuan, N., Wang, G., Wu, F., Feng, L., Luo, M., Li, M., Luo, A., Zhao, X., Zhang, L."Cellular communication promotes mammosphere growth and collective invasion through microtubule‑like structures and angiogenesis". Oncology Reports 40, no. 6 (2018): 3297-3312. https://doi.org/10.3892/or.2018.6778
Copy and paste a formatted citation
x
Spandidos Publications style
Huang S, Yuan N, Wang G, Wu F, Feng L, Luo M, Li M, Luo A, Zhao X, Zhang L, Zhang L, et al: Cellular communication promotes mammosphere growth and collective invasion through microtubule‑like structures and angiogenesis. Oncol Rep 40: 3297-3312, 2018.
APA
Huang, S., Yuan, N., Wang, G., Wu, F., Feng, L., Luo, M. ... Zhang, L. (2018). Cellular communication promotes mammosphere growth and collective invasion through microtubule‑like structures and angiogenesis. Oncology Reports, 40, 3297-3312. https://doi.org/10.3892/or.2018.6778
MLA
Huang, S., Yuan, N., Wang, G., Wu, F., Feng, L., Luo, M., Li, M., Luo, A., Zhao, X., Zhang, L."Cellular communication promotes mammosphere growth and collective invasion through microtubule‑like structures and angiogenesis". Oncology Reports 40.6 (2018): 3297-3312.
Chicago
Huang, S., Yuan, N., Wang, G., Wu, F., Feng, L., Luo, M., Li, M., Luo, A., Zhao, X., Zhang, L."Cellular communication promotes mammosphere growth and collective invasion through microtubule‑like structures and angiogenesis". Oncology Reports 40, no. 6 (2018): 3297-3312. https://doi.org/10.3892/or.2018.6778
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