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Exosomal microRNAs as potential biomarkers for cancer cell migration and prognosis in hepatocellular carcinoma patient-derived cell models

  • Authors:
    • Ling-Xiang Yu
    • Bo-Lun Zhang
    • Yuan Yang
    • Meng-Chao Wang
    • Guang-Lin Lei
    • Yuan Gao
    • Hu Liu
    • Chao-Hui Xiao
    • Jia-Jia Xu
    • Hao Qin
    • Xiao-Ya Xu
    • Zi-Shuo Chen
    • Da-Dong Zhang
    • Fu-Gen Li
    • Shao-Geng Zhang
    • Rong Liu
  • View Affiliations / Copyright

    Affiliations: Department of Surgical Oncology, Chinese People's Liberation Army (PLA) General Hospital, Beijing 100853, P.R. China, Department of General Surgery, Clinical Medical College of Weifang Medical University, Weifang, Shandong 261053, P.R. China, The Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, P.R. China, Department of Hepatobiliary Surgery, Hospital 302 of the PLA, Beijing 100039, P.R. China, Research and Development Institute of Precision Medicine, 3D Medicine Inc., Shanghai 201114, P.R. China
    Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 257-269
    |
    Published online on: October 29, 2018
       https://doi.org/10.3892/or.2018.6829
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Abstract

Hepatocellular carcinoma (HCC) is one of the most common malignant tumors worldwide, and current treatments exhibit limited efficacy against advanced HCC. The majority of cancer-related deaths are caused by metastasis from the primary tumor, which indicates the importance of identifying clinical biomarkers for predicting metastasis and indicating prognosis. Patient-derived cells (PDCs) may be effective models for biomarker identification. In the present study, a wound healing assay was used to obtain 10 fast-migrated and 10 slow-migrated PDC cultures from 36 HCC samples. MicroRNA (miRNA) signatures in PDCs and PDC-derived exosomes were profiled by microRNA-sequencing. Differentially expressed miRNAs between the low- and fast-migrated groups were identified and further validated in 372 HCC profiles from The Cancer Genome Atlas (TCGA). Six exosomal miRNAs were identified to be differentially expressed between the two groups. In the fast-migrated group, five miRNAs (miR-140-3p, miR-30d-5p, miR-29b-3p, miR-130b-3p and miR-330-5p) were downregulated, and one miRNA (miR-296-3p) was upregulated compared with the slow-migrated group. Pathway analysis demonstrated that the target genes of the differentially expressed miRNAs were significantly enriched in the ‘focal adhesion’ pathway, which is consistent with the roles of these miRNAs in tumor metastasis. Three miRNAs, miR-30d, miR-140 and miR-29b, were significantly associated with patient survival. These findings indicated that these exosomal miRNAs may be candidate biomarkers for predicting HCC cell migration and prognosis and may guide the treatment of advanced HCC.
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Copy and paste a formatted citation
Spandidos Publications style
Yu L, Zhang B, Yang Y, Wang M, Lei G, Gao Y, Liu H, Xiao C, Xu J, Qin H, Qin H, et al: Exosomal microRNAs as potential biomarkers for cancer cell migration and prognosis in hepatocellular carcinoma patient-derived cell models. Oncol Rep 41: 257-269, 2019.
APA
Yu, L., Zhang, B., Yang, Y., Wang, M., Lei, G., Gao, Y. ... Liu, R. (2019). Exosomal microRNAs as potential biomarkers for cancer cell migration and prognosis in hepatocellular carcinoma patient-derived cell models. Oncology Reports, 41, 257-269. https://doi.org/10.3892/or.2018.6829
MLA
Yu, L., Zhang, B., Yang, Y., Wang, M., Lei, G., Gao, Y., Liu, H., Xiao, C., Xu, J., Qin, H., Xu, X., Chen, Z., Zhang, D., Li, F., Zhang, S., Liu, R."Exosomal microRNAs as potential biomarkers for cancer cell migration and prognosis in hepatocellular carcinoma patient-derived cell models". Oncology Reports 41.1 (2019): 257-269.
Chicago
Yu, L., Zhang, B., Yang, Y., Wang, M., Lei, G., Gao, Y., Liu, H., Xiao, C., Xu, J., Qin, H., Xu, X., Chen, Z., Zhang, D., Li, F., Zhang, S., Liu, R."Exosomal microRNAs as potential biomarkers for cancer cell migration and prognosis in hepatocellular carcinoma patient-derived cell models". Oncology Reports 41, no. 1 (2019): 257-269. https://doi.org/10.3892/or.2018.6829
Copy and paste a formatted citation
x
Spandidos Publications style
Yu L, Zhang B, Yang Y, Wang M, Lei G, Gao Y, Liu H, Xiao C, Xu J, Qin H, Qin H, et al: Exosomal microRNAs as potential biomarkers for cancer cell migration and prognosis in hepatocellular carcinoma patient-derived cell models. Oncol Rep 41: 257-269, 2019.
APA
Yu, L., Zhang, B., Yang, Y., Wang, M., Lei, G., Gao, Y. ... Liu, R. (2019). Exosomal microRNAs as potential biomarkers for cancer cell migration and prognosis in hepatocellular carcinoma patient-derived cell models. Oncology Reports, 41, 257-269. https://doi.org/10.3892/or.2018.6829
MLA
Yu, L., Zhang, B., Yang, Y., Wang, M., Lei, G., Gao, Y., Liu, H., Xiao, C., Xu, J., Qin, H., Xu, X., Chen, Z., Zhang, D., Li, F., Zhang, S., Liu, R."Exosomal microRNAs as potential biomarkers for cancer cell migration and prognosis in hepatocellular carcinoma patient-derived cell models". Oncology Reports 41.1 (2019): 257-269.
Chicago
Yu, L., Zhang, B., Yang, Y., Wang, M., Lei, G., Gao, Y., Liu, H., Xiao, C., Xu, J., Qin, H., Xu, X., Chen, Z., Zhang, D., Li, F., Zhang, S., Liu, R."Exosomal microRNAs as potential biomarkers for cancer cell migration and prognosis in hepatocellular carcinoma patient-derived cell models". Oncology Reports 41, no. 1 (2019): 257-269. https://doi.org/10.3892/or.2018.6829
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