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Article

Upregulation and hypomethylation of lncRNA AFAP1‑AS1 predicts a poor prognosis and promotes the migration and invasion of cervical cancer

  • Authors:
    • Hao Bo
    • Liqing Fan
    • Zhaojian Gong
    • Zhizhong Liu
    • Lei Shi
    • Can Guo
    • Xiayu Li
    • Qianjin Liao
    • Wenling Zhang
    • Ming Zhou
    • Bo Xiang
    • Xiaoling Li
    • Guiyuan Li
    • Wei Xiong
    • Zhaoyang Zeng
    • Ke Cao
    • Shanshan Zhang
    • Fang Xiong
  • View Affiliations / Copyright

    Affiliations: The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, Hunan 410000, P.R. China, Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, Hunan 410000, P.R. China, Department of Oral and Maxillofacial Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410000, P.R. China, Hunan Key Laboratory of Translational Radiation Oncology, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan 410000, P.R. China, The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medical Science, Central South University, Changsha, Hunan 410000, P.R. China, Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Department of Oncology, Third Xiangya Hospital, Central South University, Changsha, Hunan 410000, P.R. China
  • Pages: 2431-2439
    |
    Published online on: February 21, 2019
       https://doi.org/10.3892/or.2019.7027
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Abstract

Although lncRNA AFAP1 antisense RNA1 (AFAP1‑AS1) is considered an oncogenic lncRNA, little is known about the role of AFAP1‑AS1 in cervical cancer. In the present study, we found that AFAP1‑AS1 was elevated and hypomethylated in cervical cancer and was associated with a poor prognosis of patients with cervical cancer by analyzing the Cancer RNA‑Seq Nexus (CRN), MethCH and UCSC XENA databases. Subsequently, we knocked AFAP1‑AS1 expression down using siRNAs in cervical cancer cells. Wound healing experiments and matrigel invasion experiments revealed that the downregulation of AFAP1‑AS1 suppressed the migration and invasion of cervical cancer cells. Furthermore, western blot analysis demonstrated that the antitumor effects induced by the silencing of AFAP1‑AS1 were mainly mediated through the regulation of the Rho/Rac signaling pathway and epithelial‑mesenchymal transition (EMT)‑related genes. Taken together, the findings of the present study indicate that the expression level of AFAP1‑AS1 may be involved in the development of cervical cancer. Thus, AFAP1‑AS1 may be a novel prognostic biomarker and a potential therapeutic target for patients with cervical cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Bo H, Fan L, Gong Z, Liu Z, Shi L, Guo C, Li X, Liao Q, Zhang W, Zhou M, Zhou M, et al: Upregulation and hypomethylation of lncRNA AFAP1‑AS1 predicts a poor prognosis and promotes the migration and invasion of cervical cancer. Oncol Rep 41: 2431-2439, 2019.
APA
Bo, H., Fan, L., Gong, Z., Liu, Z., Shi, L., Guo, C. ... Xiong, F. (2019). Upregulation and hypomethylation of lncRNA AFAP1‑AS1 predicts a poor prognosis and promotes the migration and invasion of cervical cancer. Oncology Reports, 41, 2431-2439. https://doi.org/10.3892/or.2019.7027
MLA
Bo, H., Fan, L., Gong, Z., Liu, Z., Shi, L., Guo, C., Li, X., Liao, Q., Zhang, W., Zhou, M., Xiang, B., Li, X., Li, G., Xiong, W., Zeng, Z., Cao, K., Zhang, S., Xiong, F."Upregulation and hypomethylation of lncRNA AFAP1‑AS1 predicts a poor prognosis and promotes the migration and invasion of cervical cancer". Oncology Reports 41.4 (2019): 2431-2439.
Chicago
Bo, H., Fan, L., Gong, Z., Liu, Z., Shi, L., Guo, C., Li, X., Liao, Q., Zhang, W., Zhou, M., Xiang, B., Li, X., Li, G., Xiong, W., Zeng, Z., Cao, K., Zhang, S., Xiong, F."Upregulation and hypomethylation of lncRNA AFAP1‑AS1 predicts a poor prognosis and promotes the migration and invasion of cervical cancer". Oncology Reports 41, no. 4 (2019): 2431-2439. https://doi.org/10.3892/or.2019.7027
Copy and paste a formatted citation
x
Spandidos Publications style
Bo H, Fan L, Gong Z, Liu Z, Shi L, Guo C, Li X, Liao Q, Zhang W, Zhou M, Zhou M, et al: Upregulation and hypomethylation of lncRNA AFAP1‑AS1 predicts a poor prognosis and promotes the migration and invasion of cervical cancer. Oncol Rep 41: 2431-2439, 2019.
APA
Bo, H., Fan, L., Gong, Z., Liu, Z., Shi, L., Guo, C. ... Xiong, F. (2019). Upregulation and hypomethylation of lncRNA AFAP1‑AS1 predicts a poor prognosis and promotes the migration and invasion of cervical cancer. Oncology Reports, 41, 2431-2439. https://doi.org/10.3892/or.2019.7027
MLA
Bo, H., Fan, L., Gong, Z., Liu, Z., Shi, L., Guo, C., Li, X., Liao, Q., Zhang, W., Zhou, M., Xiang, B., Li, X., Li, G., Xiong, W., Zeng, Z., Cao, K., Zhang, S., Xiong, F."Upregulation and hypomethylation of lncRNA AFAP1‑AS1 predicts a poor prognosis and promotes the migration and invasion of cervical cancer". Oncology Reports 41.4 (2019): 2431-2439.
Chicago
Bo, H., Fan, L., Gong, Z., Liu, Z., Shi, L., Guo, C., Li, X., Liao, Q., Zhang, W., Zhou, M., Xiang, B., Li, X., Li, G., Xiong, W., Zeng, Z., Cao, K., Zhang, S., Xiong, F."Upregulation and hypomethylation of lncRNA AFAP1‑AS1 predicts a poor prognosis and promotes the migration and invasion of cervical cancer". Oncology Reports 41, no. 4 (2019): 2431-2439. https://doi.org/10.3892/or.2019.7027
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