LncRNA‑AB209371 promotes the epithelial‑mesenchymal transition of hepatocellular carcinoma cells

  • Authors:
    • Chaocheng Xiao
    • Xinqiang Wan
    • Haiyang Yu
    • Xiaotong Chen
    • Xiangxiang Shan
    • Yufeng Miao
    • Rengen Fan
    • Wenzhang Cha
  • View Affiliations

  • Published online on: March 7, 2019     https://doi.org/10.3892/or.2019.7045
  • Pages: 2957-2966
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Abstract

The zinc finger protein Snail1 is an important factor in the regulation of the epithelial‑mesenchymal transition (EMT) of hepatocellular carcinoma (HCC) cells. The present study demonstrated that the expression of Snail1 in HCC tissues was significantly higher compared with its expression in tissues adjacent to primary sites, as determined via western blotting. Furthermore, the results of a dual luciferase assay revealed that hsa‑microRNA(miR)199a‑5p negatively regulated the protein expression of Snail1 by binding to its 3' untranslated region. However, in a comparative analysis of primary HCC and its metastatic tissues using reverse transcription‑quantitative polymerase chain reaction and western blotting, it was demonstrated that the expression of hsa‑miR199a‑5p and Snail1 in HCC metastatic tissues were significantly higher compared with primary lesions and an association between them identified that hsa‑miR199a‑5p lost its ability to negatively regulate Snail1. This result is contradictive to the fact that hsa‑miR199a‑5p inhibits the expression of the Snail1 protein. The present study hypothesized that the aberrant expression of long non‑coding RNA was the cause of hsa‑miR199a‑5p inactivation based on loss of function rather than a reduction in content. The data collected in the present study confirmed the hypothesis that AB209371 binds to hsa‑miR199a‑5p and weakened the inhibitory effect of hsa‑miR199a‑5p on Snail1 expression. In addition, an in vitro EMT model was established in the present study by inducing HCC cells with TGF‑β1. The results revealed that AB209371 silencing effectively reversed the hsa‑miR199a‑5p mediated inhibition of EMT by negatively regulating Snail1 protein expression. Therefore, AB209371 silencing in combination with hsa‑miR199a‑5p expression may serve as an effective means to inhibit EMT in HCC cells. The present study also revealed that hsa‑miR199a‑5p/Snail1 exhibits a dominant regulatory effect in the EMT of HCC cells via a Snail1 recovery experiment. In conclusion, to the best of our knowledge, the present study confirmed for the first time that the high expression of AB209371 is favorable for the EMT in HCC cells and may be a direct cause of hsa‑miR199a‑5p inactivation (an HCC metastasis suppressor). Additionally, AB209371 silencing combined with hsa‑miR199a‑5p overexpression may be an effective means to inhibit the metastasis of HCC and the EMT of HCC cells.
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May-2019
Volume 41 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Xiao C, Wan X, Yu H, Chen X, Shan X, Miao Y, Fan R and Cha W: LncRNA‑AB209371 promotes the epithelial‑mesenchymal transition of hepatocellular carcinoma cells. Oncol Rep 41: 2957-2966, 2019
APA
Xiao, C., Wan, X., Yu, H., Chen, X., Shan, X., Miao, Y. ... Cha, W. (2019). LncRNA‑AB209371 promotes the epithelial‑mesenchymal transition of hepatocellular carcinoma cells. Oncology Reports, 41, 2957-2966. https://doi.org/10.3892/or.2019.7045
MLA
Xiao, C., Wan, X., Yu, H., Chen, X., Shan, X., Miao, Y., Fan, R., Cha, W."LncRNA‑AB209371 promotes the epithelial‑mesenchymal transition of hepatocellular carcinoma cells". Oncology Reports 41.5 (2019): 2957-2966.
Chicago
Xiao, C., Wan, X., Yu, H., Chen, X., Shan, X., Miao, Y., Fan, R., Cha, W."LncRNA‑AB209371 promotes the epithelial‑mesenchymal transition of hepatocellular carcinoma cells". Oncology Reports 41, no. 5 (2019): 2957-2966. https://doi.org/10.3892/or.2019.7045