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Article Open Access

PD‑L1 promotes head and neck squamous cell carcinoma cell growth through mTOR signaling

  • Authors:
    • Anyuan Zheng
    • Fen Li
    • Fuhai Chen
    • Jingjing Zuo
    • Lei Wang
    • Yongping Wang
    • Shiming Chen
    • Bokui Xiao
    • Zezhang Tao
  • View Affiliations / Copyright

    Affiliations: Department of Otolaryngology‑Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China, Research Institute of Otolaryngology‑Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China
    Copyright: © Zheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2833-2843
    |
    Published online on: March 7, 2019
       https://doi.org/10.3892/or.2019.7053
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Abstract

Programmed death‑ligand 1 (PD‑L1), an immune co‑stimulatory molecule, is expressed on various cancer cells and the surface of immune cells. Its overexpression on tumor cells suppresses the immune response to promote tumor cell immune escape. The present study demonstrated that PD‑L1 was critical in head and neck squamous cell carcinoma (HNSCC) carcinogenesis. Immunohistochemical analysis of HNSCC tissue microarrays revealed that PD‑L1 was overexpressed in tumor tissue, and its expression increased as tumor malignancy progressed (from grade I to IV). Subsequently, the expression of PD‑L1 was knocked down or overexpressed in the HNSCC cell lines Cal‑27 and Fadu. It was demonstrated that PD‑L1 significantly induced HNSCC cell proliferation and colony forming ability. Cell proliferation was also promoted in Cal‑27 cell xenograft BALB/c nude mice. In addition, it was determined by western blotting that the PD‑L1‑mediated increase in HNSCC cell proliferation may have been associated with the activation of mammalian target of rapamycin (mTOR) signaling pathway. Furthermore, mTOR inhibitor (rapamycin) prevented the increase in proliferation. Based on these results, it was concluded that PD‑L1 promoted cell proliferation of HNSCC cells through mTOR signaling, and blocking PD‑L1 may be conducive in HNSCC therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Zheng A, Li F, Chen F, Zuo J, Wang L, Wang Y, Chen S, Xiao B and Tao Z: PD‑L1 promotes head and neck squamous cell carcinoma cell growth through mTOR signaling. Oncol Rep 41: 2833-2843, 2019.
APA
Zheng, A., Li, F., Chen, F., Zuo, J., Wang, L., Wang, Y. ... Tao, Z. (2019). PD‑L1 promotes head and neck squamous cell carcinoma cell growth through mTOR signaling. Oncology Reports, 41, 2833-2843. https://doi.org/10.3892/or.2019.7053
MLA
Zheng, A., Li, F., Chen, F., Zuo, J., Wang, L., Wang, Y., Chen, S., Xiao, B., Tao, Z."PD‑L1 promotes head and neck squamous cell carcinoma cell growth through mTOR signaling". Oncology Reports 41.5 (2019): 2833-2843.
Chicago
Zheng, A., Li, F., Chen, F., Zuo, J., Wang, L., Wang, Y., Chen, S., Xiao, B., Tao, Z."PD‑L1 promotes head and neck squamous cell carcinoma cell growth through mTOR signaling". Oncology Reports 41, no. 5 (2019): 2833-2843. https://doi.org/10.3892/or.2019.7053
Copy and paste a formatted citation
x
Spandidos Publications style
Zheng A, Li F, Chen F, Zuo J, Wang L, Wang Y, Chen S, Xiao B and Tao Z: PD‑L1 promotes head and neck squamous cell carcinoma cell growth through mTOR signaling. Oncol Rep 41: 2833-2843, 2019.
APA
Zheng, A., Li, F., Chen, F., Zuo, J., Wang, L., Wang, Y. ... Tao, Z. (2019). PD‑L1 promotes head and neck squamous cell carcinoma cell growth through mTOR signaling. Oncology Reports, 41, 2833-2843. https://doi.org/10.3892/or.2019.7053
MLA
Zheng, A., Li, F., Chen, F., Zuo, J., Wang, L., Wang, Y., Chen, S., Xiao, B., Tao, Z."PD‑L1 promotes head and neck squamous cell carcinoma cell growth through mTOR signaling". Oncology Reports 41.5 (2019): 2833-2843.
Chicago
Zheng, A., Li, F., Chen, F., Zuo, J., Wang, L., Wang, Y., Chen, S., Xiao, B., Tao, Z."PD‑L1 promotes head and neck squamous cell carcinoma cell growth through mTOR signaling". Oncology Reports 41, no. 5 (2019): 2833-2843. https://doi.org/10.3892/or.2019.7053
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