Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1021-335X Online ISSN: 1791-2431
Journal Cover
May-2019 Volume 41 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
May-2019 Volume 41 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

LASS2 inhibits proliferation and induces apoptosis in HepG2 cells by affecting mitochondrial dynamics, the cell cycle and the nuclear factor‑κB pathways

  • Authors:
    • Yan Yang
    • Xiaoli Yang
    • Lin Li
    • Gangyi Yang
    • Xuhong Ouyang
    • Jialin Xiang
    • Tao Zhang
    • Xun Min
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou 563003, P.R. China, The Key Laboratory of Medical Diagnostics in the Ministry of Education and Department of Clinical Biochemistry, College of Laboratory Medicine, Chongqing Medical University, Chongqing, Sichuan 400010, P.R. China, Department of Endocrinology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, Sichuan 400010, P.R. China
  • Pages: 3005-3014
    |
    Published online on: March 13, 2019
       https://doi.org/10.3892/or.2019.7058
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

LAG1 longevity assurance homolog 2 (LASS2) is a candidate biomarker in cancer that is dysregulated in various types of tumor, potentially affecting cell growth, invasion and migration. Although its effects on liver cancer metastasis and invasion have been reported, specific phenotypic studies and potential molecular mechanisms have not been completely elucidated in hepatoblastoma (HB). In the present study, the effect of LASS2 on the proliferation, apoptosis and cell cycle of HepG2 HB cells was assessed, and the underlying mechanisms were investigated. The human LASS2 coding sequence was inserted into an adenovirus vector and transduced into HepG2 cells. It was determined that the overexpression of LASS2 inhibited HepG2 cell viability and proliferation, as determined by cell counting kit‑8 and colony formation assays, and induced apoptosis by increasing reactive oxygen species, reducing mitochondrial membrane potential and inducing intracellular Ca2+ overload. In addition, the overexpression of LASS2 induced G0/G1 cell cycle arrest through modulating the expression of cell cycle regulatory proteins, including p27, cyclin D1 and cyclin‑dependent kinase 4. Immunofluorescence was used to determine that nuclear factor (NF)‑κB p‑p65 was primarily expressed in the cytoplasm rather than in the nucleus; western blot analysis demonstrated that LASS2 downregulated the expression of NF‑κB p‑p65 relative to its inactive form in HepG2 cells. These findings suggest that LASS2 inhibits proliferation and induces apoptosis in HepG2 HB cells through the mitochondrial apoptotic, NF‑κB and cell cycle signaling pathways.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Bosch FX, Ribes J, Díaz M and Cléries R: Primary liver cancer: Worldwide incidence and trends. Gastroenterology. 127 (Suppl 1):S5–S16. 2004. View Article : Google Scholar : PubMed/NCBI

2 

Bruix J, Han KH, Gores G, Llovet JM and Mazzaferro V: Liver cancer: Approaching a personalized care. J Hepatol. 62 (Suppl):S144–S156. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Yan X and Qiu Y: Impact of current staging systems on treatment strategy for HBV-related hepatocellular carcinoma. Cancer Lett. 379:220–224. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Sia D, Villanueva A, Friedman SL and Llovet JM: Liver cancer cell of origin, molecular class, and effects on patient prognosis. Gastroenterology. 152:745–761. 2017. View Article : Google Scholar : PubMed/NCBI

5 

Eichenmüller M, Gruner I, Hagl B, Häberle B, Müller-Höcker J, von Schweinitz D and Kappler R: Blocking the hedgehog pathway inhibits hepatoblastoma growth. Hepatology. 49:482–490. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Beck A, Trippel F, Wagner A, Joppien S, Felle M, Vokuhl C, Schwarzmayr T, Strom TM, von Schweinitz D, Längst G, et al: Overexpression of UHRF1 promotes silencing of tumor suppressor genes and predicts outcome in hepatoblastoma. Clin Epigenetics. 10:272018. View Article : Google Scholar : PubMed/NCBI

7 

Wu JF, Chang HH, Lu MY, Jou ST, Chang KC, Ni YH and Chang MH: Prognostic roles of pathology markers immunoexpression and clinical parameters in hepatoblastoma. J Biomed Sci. 24:622017. View Article : Google Scholar : PubMed/NCBI

8 

Mei F, You J, Liu B, Zhang M, Liu J, Zhang B and Pei F: LASS2/TMSG1 inhibits growth and invasion of breast cancer cell in vitro through regulation of vacuolar ATPase activity. Tumour Biol. 36:2831–2844. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Mizutani Y, Kihara A and Igarashi Y: Mammalian Lass6 and its related family members regulate synthesis of specific ceramides. Biochem J. 390:263–271. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Spassieva SD, Mullen TD, Townsend DM and Obeid LM: Disruption of ceramide synthesis by CerS2 down-regulation leads to autophagy and the unfolded protein response. Biochem J. 424:273–283. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Gu D, Jin H, Jin G, Wang C, Wang N, Hu F, Luo Q, Chu W, Yao M and Qin W: The asialoglycoprotein receptor suppresses the metastasis of hepatocellular carcinoma via LASS2-mediated inhibition of V-ATPase activity. Cancer Lett. 379:107–116. 2016. View Article : Google Scholar : PubMed/NCBI

12 

Tang N, Jin J, Deng Y, Ke RH, Shen QJ, Fan SH and Qin WX: [LASS2 interacts with V-ATPase and inhibits cell growth of hepatocellular carcinoma]. Sheng Li Xue Bao. 62:196–202. 2010.(In Chinese). PubMed/NCBI

13 

Huang L, Luan T, Chen Y, Bao X, Huang Y, Fu S, Wang H and Wang J: LASS2 regulates invasion and chemoresistance via ERK/Drp1 modulated mitochondrial dynamics in bladder cancer cells. J Cancer. 9:1017–1024. 2018. View Article : Google Scholar : PubMed/NCBI

14 

Wang H, Zuo Y, Ding M, Ke C, Yan R, Zhan H, Liu J, Wang W, Li N and Wang J: LASS2 inhibits growth and invasion of bladder cancer by regulating ATPase activity. Oncol Lett. 13:661–668. 2017. View Article : Google Scholar : PubMed/NCBI

15 

Fan S, Niu Y, Tan N, Wu Z, Wang Y, You H, Ke R, Song J, Shen Q, Wang W, et al: LASS2 enhances chemosensitivity of breast cancer by counteracting acidic tumor microenvironment through inhibiting activity of V-ATPase proton pump. Oncogene. 32:1682–1690. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Xu X, You J and Pei F: Silencing of a novel tumor metastasis suppressor gene LASS2/TMSG1 promotes invasion of prostate cancer cell in vitro through increase of vacuolar ATPase activity. J Cell Biochem. 113:2356–2363. 2012. View Article : Google Scholar : PubMed/NCBI

17 

Ke RH, Wang Y, Mao Y, Zhang J and Xiong J: Decreased expression of LASS2 is associated with worse prognosis in meningiomas. J Neurooncol. 118:369–376. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Ruan H, Wang T, Yang C, Jin G, Gu D, Deng X, Wang C, Qin W and Jin H: Co-expression of LASS2 and TGF-β1 predicts poor prognosis in hepatocellular carcinoma. Sci Rep. 6:324212016. View Article : Google Scholar : PubMed/NCBI

19 

López-Terrada D, Cheung SW, Finegold MJ and Knowles BB: Hep G2 is a hepatoblastoma-derived cell line. Hum Pathol. 40:1512–1515. 2009. View Article : Google Scholar

20 

Zhang ZL, Liu GC, Peng L, Zhang C, Jia YM, Yang WH and Mao L: Effect of PAK1 gene silencing on proliferation and apoptosis in hepatocellular carcinoma cell lines MHCC97-H and HepG2 and cells in xenograft tumor. Gene Ther. 25:284–296. 2018. View Article : Google Scholar : PubMed/NCBI

21 

Ceballos MP, Decándido G, Quiroga AD, Comanzo CG, Livore VI, Lorenzetti F, Lambertucci F, Chazarreta-Cifre L, Banchio C, Alvarez ML, et al: Inhibition of sirtuins 1 and 2 impairs cell survival and migration and modulates the expression of P-glycoprotein and MRP3 in hepatocellular carcinoma cell lines. Toxicol Lett. 289:63–74. 2018. View Article : Google Scholar : PubMed/NCBI

22 

Dunham KR and Selman BR: Interactions of inorganic phosphate with spinach coupling factor 1. Effects on ATPase and ADP binding activities. J Biol Chem. 256:10044–10049. 1981.PubMed/NCBI

23 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

24 

Mankan AK, Lawless MW, Gray SG, Kelleher D and McManus R: NF-kappaB regulation: The nuclear response. J Cell Mol Med. 13:631–643. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Pantano C, Reynaert NL, van der Vliet A and Janssen-Heininger YM: Redox-sensitive kinases of the nuclear factor-kappaB signaling pathway. Antioxid Redox Signal. 8:1791–1806. 2006. View Article : Google Scholar : PubMed/NCBI

26 

Zeng GZ, Wang Z, Zhao LM, Fan JT and Tan NH: NF-κB and JNK mediated apoptosis and G0/G1 arrest of HeLa cells induced by rubiarbonol G, an arborinane-type triterpenoid from Rubia yunnanensis. J Ethnopharmacol. 220:220–227. 2018. View Article : Google Scholar : PubMed/NCBI

27 

Li W, Zhi W, Zhao J, Yao Q, Liu F and Niu X: Cinnamaldehyde protects VSMCs against ox-LDL-induced proliferation and migration through S arrest and inhibition of p38, JNK/MAPKs and NF-κB. Vascul Pharmacol. 108:57–66. 2018. View Article : Google Scholar : PubMed/NCBI

28 

Ye D, Luo H, Lai Z, Zou L, Zhu L, Mao J, Jacob T, Ye W, Wang L and Chen L: ClC-3 chloride channel proteins regulate the cell cycle by up-regulating cyclin D1-CDK4/6 through suppressing p21/p27 expression in nasopharyngeal carcinoma cells. Sci Rep. 6:302762016. View Article : Google Scholar : PubMed/NCBI

29 

James MK, Ray A, Leznova D and Blain SW: Differential modification of p27Kip1 controls its cyclin D-cdk4 inhibitory activity. Mol Cell Biol. 28:498–510. 2008. View Article : Google Scholar : PubMed/NCBI

30 

Philipp-Staheli J, Payne SR and Kemp CJ: p27(Kip1): Regulation and function of a haploinsufficient tumor suppressor and its misregulation in cancer. Exp Cell Res. 264:148–168. 2001. View Article : Google Scholar : PubMed/NCBI

31 

Caro AA, Evans KL and Cederbaum AI: CYP2E1 overexpression inhibits microsomal Ca2+-ATPase activity in HepG2 cells. Toxicology. 255:171–176. 2009. View Article : Google Scholar : PubMed/NCBI

32 

Zong WX, Rabinowitz JD and White E: Mitochondria and Cancer. Mol Cell. 61:667–676. 2016. View Article : Google Scholar : PubMed/NCBI

33 

Xu X, Liu B, Zou P, Zhang Y, You J and Pei F: Silencing of LASS2/TMSG1 enhances invasion and metastasis capacity of prostate cancer cell. J Cell Biochem. 115:731–743. 2014. View Article : Google Scholar : PubMed/NCBI

34 

Su J, Yu W, Gong M, You J, Liu J and Zheng J: Overexpression of a novel tumor metastasis suppressor gene TMSG1/LASS2 induces apoptosis via a caspase-dependent mitochondrial pathway. J Cell Biochem. 116:1310–1317. 2015. View Article : Google Scholar : PubMed/NCBI

35 

Hengartner MO: The biochemistry of apoptosis. Nature. 407:770–776. 2000. View Article : Google Scholar : PubMed/NCBI

36 

Krammer PH, Kamiński M, Kiessling M and Gülow K: No life without death. Adv Cancer Res. 97:111–138. 2007. View Article : Google Scholar : PubMed/NCBI

37 

Gupta AK, Ghosh K, Palit S, Barua J, Das PK and Ukil A: Leishmania donovani inhibits inflammasome-dependent macrophage activation by exploiting the negative regulatory proteins A20 and UCP2. FASEB J. 31:5087–5101. 2017. View Article : Google Scholar : PubMed/NCBI

38 

Ko EY, Cho SH, Kwon SH, Eom CY, Jeong MS, Lee W, Kim SY, Heo SJ, Ahn G, Lee KP, et al: The roles of NF-κB and ROS in regulation of pro-inflammatory mediators of inflammation induction in LPS-stimulated zebrafish embryos. Fish Shellfish Immunol. 68:525–529. 2017. View Article : Google Scholar : PubMed/NCBI

39 

Pannunzio NR and Lieber MR: AID and reactive oxygen species can induce DNA breaks within human chromosomal translocation fragile zones. Mol Cell. 68:901–912.e3. 2017. View Article : Google Scholar : PubMed/NCBI

40 

Zou Z, Chang H, Li H and Wang S: Induction of reactive oxygen species: An emerging approach for cancer therapy. Apoptosis. 22:1321–1335. 2017. View Article : Google Scholar : PubMed/NCBI

41 

Hsu YC, Huang TY and Chen MJ: Therapeutic ROS targeting of GADD45γ in the induction of G2/M arrest in primary human colorectal cancer cell lines by cucurbitacin E. Cell Death Dis. 5:e11982014. View Article : Google Scholar : PubMed/NCBI

42 

Park WH: H2O2 inhibits the growth of human pulmonary fibroblast cells by inducing cell death, GSH depletion and G1 phase arrest. Mol Med Rep. 7:1235–1240. 2013. View Article : Google Scholar : PubMed/NCBI

43 

Kim J, Kim SH, Johnson VJ and Sharma RP: Extracellular signal-regulated kinase-signaling-dependent G2/M arrest and cell death in murine macrophages by cadmium. Environ Toxicol Chem. 24:3069–3077. 2005. View Article : Google Scholar : PubMed/NCBI

44 

Kalghatgi S, Spina CS, Costello JC, Liesa M, Morones-Ramirez JR, Slomovic S, Molina A, Shirihai OS and Collins JJ: Bactericidal antibiotics induce mitochondrial dysfunction and oxidative damage in Mammalian cells. Sci Transl Med. 5:192ra852013. View Article : Google Scholar : PubMed/NCBI

45 

Zhang S, Li T, Zhang L, Wang X, Dong H, Li L, Fu D, Li Y, Zi X, Liu HM, et al: A novel chalcone derivative S17 induces apoptosis through ROS dependent DR5 up-regulation in gastric cancer cells. Sci Rep. 7:98732017. View Article : Google Scholar : PubMed/NCBI

46 

Tiwari M, Prasad S, Shrivastav TG and Chaube SK: Calcium signaling during meiotic cell cycle regulation and apoptosis in mammalian oocytes. J Cell Physiol. 232:976–981. 2017. View Article : Google Scholar : PubMed/NCBI

47 

Bagur R and Hajnóczky G: Intracellular Ca2+ sensing: Its role in calcium homeostasis and signaling. Mol Cell. 66:780–788. 2017. View Article : Google Scholar : PubMed/NCBI

48 

Zorov DB, Juhaszova M and Sollott SJ: Mitochondrial reactive oxygen species (ROS) and ROS-induced ROS release. Physiol Rev. 94:909–950. 2014. View Article : Google Scholar : PubMed/NCBI

49 

Hamanaka RB and Chandel NS: Mitochondrial reactive oxygen species regulate cellular signaling and dictate biological outcomes. Trends Biochem Sci. 35:505–513. 2010. View Article : Google Scholar : PubMed/NCBI

50 

Moon DO, Kim MO, Lee JD, Choi YH and Kim GY: Rosmarinic acid sensitizes cell death through suppression of TNF-alpha-induced NF-kappaB activation and ROS generation in human leukemia U937 cells. Cancer Lett. 288:183–191. 2010. View Article : Google Scholar : PubMed/NCBI

51 

Korn SH, Wouters EF, Vos N and Janssen-Heininger YM: Cytokine-induced activation of nuclear factor-kappa B is inhibited by hydrogen peroxide through oxidative inactivation of IkappaB kinase. J Biol Chem. 276:35693–35700. 2001. View Article : Google Scholar : PubMed/NCBI

52 

Jamaluddin M, Wang S, Boldogh I, Tian B and Brasier AR: TNF-alpha-induced NF-kappaB/RelA Ser(276) phosphorylation and enhanceosome formation is mediated by an ROS-dependent PKAc pathway. Cell Signal. 19:1419–1433. 2007. View Article : Google Scholar : PubMed/NCBI

53 

Ge QL, Liu SH, Ai ZH, Tao MF, Ma L, Wen SY, Dai M, Liu F, Liu HS, Jiang RZ, et al: RelB/NF-κB links cell cycle transition and apoptosis to endometrioid adenocarcinoma tumorigenesis. Cell Death Dis. 7:e24022016. View Article : Google Scholar : PubMed/NCBI

54 

Wolff B and Naumann M: INK4 cell cycle inhibitors direct transcriptional inactivation of NF-kappaB. Oncogene. 18:2663–2666. 1999. View Article : Google Scholar : PubMed/NCBI

55 

Cicenas J, Kalyan K, Sorokinas A, Jatulyte A, Valiunas D, Kaupinis A and Valius M: Highlights of the latest advances in research on CDK inhibitors. Cancers (Basel). 6:2224–2242. 2014. View Article : Google Scholar : PubMed/NCBI

56 

Hinz M, Krappmann D, Eichten A, Heder A, Scheidereit C and Strauss M: NF-kappaB function in growth control: Regulation of cyclin D1 expression and G0/G1-to-S-phase transition. Mol Cell Biol. 19:2690–2698. 1999. View Article : Google Scholar : PubMed/NCBI

57 

Bera A, Ghosh-Choudhury N, Dey N, Das F, Kasinath BS, Abboud HE and Choudhury GG: NFκB-mediated cyclin D1 expression by microRNA-21 influences renal cancer cell proliferation. Cell Signal. 25:2575–2586. 2013. View Article : Google Scholar : PubMed/NCBI

58 

Guo W, Liu J, Jian J, Li J, Wan Y and Huang C: IKK-β/NF-κB p65 mediates p27(Kip1) protein degradation in arsenite response. Biochem Biophys Res Commun. 447:563–568. 2014. View Article : Google Scholar : PubMed/NCBI

59 

Poon RY, Toyoshima H and Hunter T: Redistribution of the CDK inhibitor p27 between different cyclin. CDK complexes in the mouse fibroblast cell cycle and in cells arrested with lovastatin or ultraviolet irradiation. Mol Biol Cell. 6:1197–1213. 1995. View Article : Google Scholar : PubMed/NCBI

60 

Orlando S, Gallastegui E, Besson A, Abril G, Aligué R, Pujol MJ and Bachs O: p27Kip1 and p21Cip1 collaborate in the regulation of transcription by recruiting cyclin-Cdk complexes on the promoters of target genes. Nucleic Acids Res. 43:6860–6873. 2015. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Yang Y, Yang X, Li L, Yang G, Ouyang X, Xiang J, Zhang T and Min X: LASS2 inhibits proliferation and induces apoptosis in HepG2 cells by affecting mitochondrial dynamics, the cell cycle and the nuclear factor‑κB pathways. Oncol Rep 41: 3005-3014, 2019.
APA
Yang, Y., Yang, X., Li, L., Yang, G., Ouyang, X., Xiang, J. ... Min, X. (2019). LASS2 inhibits proliferation and induces apoptosis in HepG2 cells by affecting mitochondrial dynamics, the cell cycle and the nuclear factor‑κB pathways. Oncology Reports, 41, 3005-3014. https://doi.org/10.3892/or.2019.7058
MLA
Yang, Y., Yang, X., Li, L., Yang, G., Ouyang, X., Xiang, J., Zhang, T., Min, X."LASS2 inhibits proliferation and induces apoptosis in HepG2 cells by affecting mitochondrial dynamics, the cell cycle and the nuclear factor‑κB pathways". Oncology Reports 41.5 (2019): 3005-3014.
Chicago
Yang, Y., Yang, X., Li, L., Yang, G., Ouyang, X., Xiang, J., Zhang, T., Min, X."LASS2 inhibits proliferation and induces apoptosis in HepG2 cells by affecting mitochondrial dynamics, the cell cycle and the nuclear factor‑κB pathways". Oncology Reports 41, no. 5 (2019): 3005-3014. https://doi.org/10.3892/or.2019.7058
Copy and paste a formatted citation
x
Spandidos Publications style
Yang Y, Yang X, Li L, Yang G, Ouyang X, Xiang J, Zhang T and Min X: LASS2 inhibits proliferation and induces apoptosis in HepG2 cells by affecting mitochondrial dynamics, the cell cycle and the nuclear factor‑κB pathways. Oncol Rep 41: 3005-3014, 2019.
APA
Yang, Y., Yang, X., Li, L., Yang, G., Ouyang, X., Xiang, J. ... Min, X. (2019). LASS2 inhibits proliferation and induces apoptosis in HepG2 cells by affecting mitochondrial dynamics, the cell cycle and the nuclear factor‑κB pathways. Oncology Reports, 41, 3005-3014. https://doi.org/10.3892/or.2019.7058
MLA
Yang, Y., Yang, X., Li, L., Yang, G., Ouyang, X., Xiang, J., Zhang, T., Min, X."LASS2 inhibits proliferation and induces apoptosis in HepG2 cells by affecting mitochondrial dynamics, the cell cycle and the nuclear factor‑κB pathways". Oncology Reports 41.5 (2019): 3005-3014.
Chicago
Yang, Y., Yang, X., Li, L., Yang, G., Ouyang, X., Xiang, J., Zhang, T., Min, X."LASS2 inhibits proliferation and induces apoptosis in HepG2 cells by affecting mitochondrial dynamics, the cell cycle and the nuclear factor‑κB pathways". Oncology Reports 41, no. 5 (2019): 3005-3014. https://doi.org/10.3892/or.2019.7058
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team