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Gene expression alterations of human liver cancer cells following borax exposure

  • Authors:
    • Lun Wu
    • Ying Wei
    • Wen‑Bo Zhou
    • You‑Shun Zhang
    • Qin‑Hua Chen
    • Ming‑Xing Liu
    • Zheng‑Peng Zhu
    • Jiao Zhou
    • Li‑Hua Yang
    • Hong‑Mei Wang
    • Guang‑Min Wei
    • Sheng Wang
    • Zhi‑Gang Tang
  • View Affiliations / Copyright

    Affiliations: Department of Pancreatic Surgery, Renmin Hospital of Wuhan University, Wuhan University, Wuhan, Hubei 430060, P.R. China, Liver Surgery Institute of The Experiment Center of Medicine, Dongfeng Hospital, Hubei University of Medicine, Shiyan, Hubei 442001, P.R. China, Liver Surgery Institute of The Experiment Center of Medicine, Department of Hepatobiliary Surgery, Dongfeng Hospital, Hubei University of Medicine, Shiyan, Hubei 442001, Shiyan, Hubei 442001, P.R. China, Hubei Key Laboratory of Wudang Local Chinese Medicine Research, Experiment Center of Medicine, Hubei University of Medicine, Shiyan, Hubei 442001, P.R. China, Department of Pediatrics, YunXi Health for Women And Children, Children's Hospital, Maternal & Child Care and Family Planning Service Centre, Shiyan, Hubei 442600, P.R. China , Department of Pathology, Dongfeng Hospital, Hubei University of Medicine, Shiyan, Hubei 442001, P.R. China, Subject Construction Office, Dongfeng Hospital, Hubei University of Medicine, Shiyan, Hubei 442001, P.R. China
    Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 115-130
    |
    Published online on: May 23, 2019
       https://doi.org/10.3892/or.2019.7169
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Abstract

Borax is a boron compound that is becoming widely recognized for its biological effects, including lipid peroxidation, cytotoxicity, genotoxicity, antioxidant activity and potential therapeutic benefits. However, it remains unknown whether exposure of human liver cancer (HepG2) cells to borax affects the gene expression of these cells. HepG2 cells were treated with 4 mM borax for either 2 or 24 h. Gene expression analysis was performed using Affymetrix GeneChip Human Gene 2.0 ST Arrays, which was followed by gene ontology analysis and pathway analysis. The clustering result was validated using reverse transcription‑quantitative polymerase chain reaction. A cell proliferation assay was performed using Celigo Image Cytometer Instrumentation. Following this, 2‑ or 24‑h exposure to borax significantly altered the expression level of a number of genes in HepG2 cells, specifically 530 genes (384 upregulated and 146 downregulated) or 1,763 genes (1,044 upregulated and 719 downregulated) compared with the control group, respectively (≥2‑fold; P<0.05). Twenty downregulated genes were abundantly expressed in HepG2 cells under normal conditions. Furthermore, the growth of HepG2 cells was inhibited through the downregulation of PRUNE1, NBPF1, PPcaspase‑1, UPF2 and MBTPS1 (≥1.5‑fold, P<0.05). The dysregulated genes potentially serve important roles in various biological processes, including the inflammation response, stress response, cellular growth, proliferation, apoptosis and tumorigenesis/oncolysis.
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Spandidos Publications style
Wu L, Wei Y, Zhou WB, Zhang YS, Chen QH, Liu MX, Zhu ZP, Zhou J, Yang LH, Wang HM, Wang HM, et al: Gene expression alterations of human liver cancer cells following borax exposure. Oncol Rep 42: 115-130, 2019.
APA
Wu, L., Wei, Y., Zhou, W., Zhang, Y., Chen, Q., Liu, M. ... Tang, Z. (2019). Gene expression alterations of human liver cancer cells following borax exposure. Oncology Reports, 42, 115-130. https://doi.org/10.3892/or.2019.7169
MLA
Wu, L., Wei, Y., Zhou, W., Zhang, Y., Chen, Q., Liu, M., Zhu, Z., Zhou, J., Yang, L., Wang, H., Wei, G., Wang, S., Tang, Z."Gene expression alterations of human liver cancer cells following borax exposure". Oncology Reports 42.1 (2019): 115-130.
Chicago
Wu, L., Wei, Y., Zhou, W., Zhang, Y., Chen, Q., Liu, M., Zhu, Z., Zhou, J., Yang, L., Wang, H., Wei, G., Wang, S., Tang, Z."Gene expression alterations of human liver cancer cells following borax exposure". Oncology Reports 42, no. 1 (2019): 115-130. https://doi.org/10.3892/or.2019.7169
Copy and paste a formatted citation
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Spandidos Publications style
Wu L, Wei Y, Zhou WB, Zhang YS, Chen QH, Liu MX, Zhu ZP, Zhou J, Yang LH, Wang HM, Wang HM, et al: Gene expression alterations of human liver cancer cells following borax exposure. Oncol Rep 42: 115-130, 2019.
APA
Wu, L., Wei, Y., Zhou, W., Zhang, Y., Chen, Q., Liu, M. ... Tang, Z. (2019). Gene expression alterations of human liver cancer cells following borax exposure. Oncology Reports, 42, 115-130. https://doi.org/10.3892/or.2019.7169
MLA
Wu, L., Wei, Y., Zhou, W., Zhang, Y., Chen, Q., Liu, M., Zhu, Z., Zhou, J., Yang, L., Wang, H., Wei, G., Wang, S., Tang, Z."Gene expression alterations of human liver cancer cells following borax exposure". Oncology Reports 42.1 (2019): 115-130.
Chicago
Wu, L., Wei, Y., Zhou, W., Zhang, Y., Chen, Q., Liu, M., Zhu, Z., Zhou, J., Yang, L., Wang, H., Wei, G., Wang, S., Tang, Z."Gene expression alterations of human liver cancer cells following borax exposure". Oncology Reports 42, no. 1 (2019): 115-130. https://doi.org/10.3892/or.2019.7169
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