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Bile cell‑free DNA as a novel and powerful liquid biopsy for detecting somatic variants in biliary tract cancer

  • Authors:
    • Ningjia Shen
    • Dadong Zhang
    • Lei Yin
    • Yinghe Qiu
    • Jian Liu
    • Wenlong Yu
    • Xiaohui Fu
    • Bin Zhu
    • Xiaoya Xu
    • Anqi Duan
    • Zishuo Chen
    • Xiang Wang
    • Xinkai Cao
    • Teng Zhao
    • Zisong Zhou
    • Lianghe Yu
    • Hao Qin
    • Zheng Fang
    • Jing‑Yu Li
    • Yuanjin Liu
    • Lei Xiong
    • Bo Yuan
    • Fugen Li
    • Yongjie Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Biliary II, Shanghai Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, P.R. China, Research and Development Institute of Precision Medicine, 3D Medicines, Inc., Pujiang Hi‑tech Park, Shanghai 201114, P.R. China
    Copyright: © Shen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 549-560
    |
    Published online on: May 31, 2019
       https://doi.org/10.3892/or.2019.7177
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Abstract

Tissue sampling of biliary tract carcinomas (BTCs) for molecular characterization is challenging. The aim of this study was to investigate the possibility of identifying individual actionable mutations derived from bile cell‑free DNA (cfDNA) using targeted deep sequencing. Ten BTC patients, four with gallbladder carcinomas and six with cholangiocarcinomas, were enrolled in the present study. Using targeted deep sequencing with a panel of 150 tumor‑related genes, paired bile cfDNA and tumor DNA were analyzed for mutational variants individually and then compared. The present study, to the best of our knowledge, is the first to reveal that bile cfDNA is predominantly comprised of long DNA fragments, which is not the case for plasma cfDNA. Herein, paired bile cfDNA and tumors from ten BTC patients were examined using targeted deep sequencing. When comparing bile cfDNA and tumor DNA for single nucleotide variation (SNV)/insertion and deletion (Indel), the results using targeted deep sequencing revealed high sensitivity (94.7%) and specificity (99.9%). Additionally, the sensitivity of detecting a copy number variation (CNV) was 75.0%, with a specificity of 98.9%. When comparing two bile extraction methods, including percutaneous transhepatic cholangial drainage and operation, no significant difference in SNV/Indel or CNV detection sensitivity was noted. Moreover, when examining the tumor stage and incidence site, AJCC stage II and the distal bile duct both had significantly decreased CNV detection sensitivities. The present study revealed that targeted deep sequencing can reliably detect mutational variants within bile cfDNA obtained from BTC patients. These preliminary results may shed light on bile cfDNA as a promising liquid biopsy for BTC patients.
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View References

1 

Augustine MM and Fong Y: Epidemiology and risk factors of biliary tract and primary liver tumors. Surg Oncol Clin N Am. 23:171–188. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Marcano-Bonilla L, Mohamed EA, Mounajjed T and Roberts LR: Biliary tract cancers: Epidemiology, molecular pathogenesis and genetic risk associations. Chin Clin Oncol. 5:612016. View Article : Google Scholar : PubMed/NCBI

3 

Streeter OE Jr, Beron PJ and Iyer PN: Precision medicine: Genomic profiles to individualize therapy. Otolaryngol Clin North Am. 50:765–773. 2017. View Article : Google Scholar : PubMed/NCBI

4 

Hirsch FR, Scagliotti GV, Mulshine JL, Kwon R, Curran W Jr, Wu YL and Paz-Ares L: Lung cancer: Current therapies and new targeted treatments. Lancet. 389:299–311. 2017. View Article : Google Scholar : PubMed/NCBI

5 

Nakamura H, Arai Y, Totoki Y, Shirota T, Elzawahry A, Kato M, Hama N, Hosoda F, Urushidate T and Ohashi S: Genomic spectra of biliary tract cancer. Nat Genet. 47:1003–1010. 2015. View Article : Google Scholar : PubMed/NCBI

6 

Nam AR, Kim JW, Cha Y, Ha H, Park JE, Bang JH, Jin MH, Lee KH, Kim TY and Han SW: Therapeutic implication of HER2 in advanced biliary tract cancer. Oncotarget. 7:58007–58021. 2016. View Article : Google Scholar : PubMed/NCBI

7 

Sakamoto Y, Yamagishi S, Tanizawa Y, Tajimi M, Okusaka T and Ojima H: PI3K-mTOR pathway identified as a potential therapeutic target in biliary tract cancer using a newly established patient-derived cell panel assay. Jpn J Clin Oncol. 48:396–399. 2018. View Article : Google Scholar : PubMed/NCBI

8 

Rizvi S, Yamada D, Hirsova P, Bronk SF, Werneburg NW, Krishnan A, Salim W, Zhang L, Trushina E, Truty MJ and Gores GJ: A hippo and fibroblast growth factor receptor autocrine pathway in cholangiocarcinoma. J Biol Chem. 291:8031–8047. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Saha SK, Gordan JD, Kleinstiver BP, Vu P, Najem MS, Yeo JC, Shi L, Kato Y, Levin RS, Webber JT, et al: Isocitrate dehydrogenase mutations confer dasatinib hypersensitivity and SRC dependence in intrahepatic cholangiocarcinoma. Cancer Discov. 6:727–739. 2016. View Article : Google Scholar : PubMed/NCBI

10 

Wan JCM, Massie C, Garcia-Corbacho J, Mouliere F, Brenton JD, Caldas C, Pacey S, Baird R and Rosenfeld N: Liquid biopsies come of age: Towards implementation of circulating tumour DNA. Nat Rev Cancer. 17:223–238. 2017. View Article : Google Scholar : PubMed/NCBI

11 

Yang N, Li Y, Liu Z, Qin H, Du D, Cao X, Cao X, Li J, Li D, Jiang B, et al: The characteristics of ctDNA reveal the high complexity in matching the corresponding tumor tissues. BMC Cancer. 18:3192018. View Article : Google Scholar : PubMed/NCBI

12 

Siravegna G, Marsoni S, Siena S and Bardelli A: Integrating liquid biopsies into the management of cancer. Nat Rev Clin Oncol. 14:531–548. 2017. View Article : Google Scholar : PubMed/NCBI

13 

Jiang T, Ren S and Zhou C: Role of circulating-tumor DNA analysis in non-small cell lung cancer. Lung Cancer. 90:128–134. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Jiang T, Li X, Wang J, Su C, Han W, Zhao C, Wu F, Gao G, Li W, Chen X, et al: Mutational landscape of cfDNA identifies distinct molecular features associated with therapeutic response to first-line platinum-based doublet chemotherapy in patients with advanced NSCLC. Theranostics. 7:4753–4762. 2017. View Article : Google Scholar : PubMed/NCBI

15 

Su D, Zhang D, Chen K, Lu J, Wu J, Cao X, Ying L, Jin Q, Ye Y, Xie Z, et al: High performance of targeted next generation sequencing on variance detection in clinical tumor specimens in comparison with current conventional methods. J Exp Clin Cancer Res. 36:1212017. View Article : Google Scholar : PubMed/NCBI

16 

Watanabe H, Okada M, Kaji Y, Satouchi M, Sato Y, Yamabe Y, Onaya H, Endo M, Sone M and Arai Y: New response evaluation criteria in solid tumours-revised RECIST guideline (version 1.1). Gan To Kagaku Ryoho. 36:2495–2501. 2009.(In Japanese). PubMed/NCBI

17 

Ribiere SC, Léandri C, Guillaumot MA, Brezault C and Coriat R: Therapeutic advances in the management of biliary tract carcinoma. Presse Med. 47:419–422. 2018.(In French). View Article : Google Scholar : PubMed/NCBI

18 

Zill OA, Greene C, Sebisanovic D, Siew LM, Leng J, Vu M, Hendifar AE, Wang Z, Atreya CE, Kelley RK, et al: Cell-free DNA next-generation sequencing in pancreatobiliary carcinomas. Cancer Discov. 5:1040–1048. 2015. View Article : Google Scholar : PubMed/NCBI

19 

Kinugasa H, Nouso K, Ako S, Dohi C, Matsushita H, Matsumoto K, Kato H and Okada H: Liquid biopsy of bile for the molecular diagnosis of gallbladder cancer. Cancer Biol Ther. 19:934–938. 2018. View Article : Google Scholar : PubMed/NCBI

20 

Underhill HR, Kitzman JO, Hellwig S, Welker NC, Daza R, Baker DN, Gligorich KM, Rostomily RC, Bronner MP and Shendure J: Fragment length of circulating tumor DNA. PLoS Genet. 12:e10061622016. View Article : Google Scholar : PubMed/NCBI

21 

Xie D, Ren Z, Fan J and Gao Q: Genetic profiling of intrahepatic cholangiocarcinoma and its clinical implication in targeted therapy. Am J Cancer Res. 6:577–586. 2016.PubMed/NCBI

22 

Li M, Zhang Z, Li X, Ye J, Wu X, Tan Z, Liu C, Shen B, Wang XA, Wu W, et al: Whole-exome and targeted gene sequencing of gallbladder carcinoma identifies recurrent mutations in the ErbB pathway. Nat Genet. 46:872–876. 2014. View Article : Google Scholar : PubMed/NCBI

23 

Oliveira DV, Zhang S, Chen X, Calvisi DF and Andersen JB: Molecular profiling of intrahepatic cholangiocarcinoma: The search for new therapeutic targets. Expert Rev Gastroenterol Hepatol. 11:349–356. 2017. View Article : Google Scholar : PubMed/NCBI

24 

Lee H and Ross JS: The potential role of comprehensive genomic profiling to guide targeted therapy for patients with biliary cancer. Therap Adv Gastroenterol. 10:507–520. 2017. View Article : Google Scholar : PubMed/NCBI

25 

Javle M, Bekaii-Saab T, Jain A, Wang Y, Kelley RK, Wang K, Kang HC, Catenacci D, Ali S, Krishnan S, et al: Biliary cancer: Utility of next-generation sequencing for clinical management. Cancer. 122:3838–3847. 2016. View Article : Google Scholar : PubMed/NCBI

26 

Zou S, Li J, Zhou H, Frech C, Jiang X, Chu JS, Zhao X, Li Y, Li Q, Wang H, et al: Mutational landscape of intrahepatic cholangiocarcinoma. Nat Commun. 5:56962014. View Article : Google Scholar : PubMed/NCBI

27 

Chandra A, Gupta V, Rahul R, Kumar M and Maurya A: Intraoperative ultrasonography of the biliary tract using saline as a contrast agent: A fast and accurate technique to identify complex biliary anatomy. Can J Surg. 60:316–322. 2017. View Article : Google Scholar : PubMed/NCBI

28 

Cao H, Liu J, Li T, Cao G, Xu G, Zhai S, Xue J, Wang Z, Shi S and Bai W: Interventional therapy for the treatment of severe hemobilia after percutaneous transhepatic cholangial drainage: A case series. Int Surg. 98:223–228. 2013. View Article : Google Scholar : PubMed/NCBI

29 

Jin H, Pang Q, Liu H, Li Z, Wang Y, Lu Y, Zhou L, Pan H and Huang W: Prognostic value of inflammation-based markers in patients with recurrent malignant obstructive jaundice treated by reimplantation of biliary metal stents: A retrospective observational study. Medicine (Baltimore). 96:e58952017. View Article : Google Scholar : PubMed/NCBI

30 

Li X, Yu J, Liang P, Yu X, Cheng Z, Han Z, Duan S and Zheng J: Ultrasound-guided percutaneous microwave ablation assisted by three-dimensional visualization operative treatment planning system and percutaneous transhepatic cholangial drainage with intraductal chilled saline perfusion for larger hepatic hilum hepatocellular (D ≥ 3 cm): Preliminary results. Oncotarget. 8:79742–79749. 2017.PubMed/NCBI

31 

Njei B, McCarty TR, Varadarajulu S and Navaneethan U: Systematic review with meta-analysis: endoscopic retrograde cholangiopancreatography-based modalities for the diagnosis of cholangiocarcinoma in primary sclerosing cholangitis. Aliment Pharmacol Ther. 44:1139–1151. 2016. View Article : Google Scholar : PubMed/NCBI

32 

Newman AM, Bratman SV, To J, Wynne JF, Eclov NC, Modlin LA, Liu CL, Neal JW, Wakelee HA, Merritt RE, et al: An ultrasensitive method for quantitating circulating tumor DNA with broad patient coverage. Nat Med. 20:548–554. 2014. View Article : Google Scholar : PubMed/NCBI

33 

Li J, Dittmar RL, Xia S, Zhang H, Du M, Huang CC, Druliner BR, Boardman L and Wang L: Cell-free DNA copy number variations in plasma from colorectal cancer patients. Mol Oncol. 11:1099–1111. 2017. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Shen N, Zhang D, Yin L, Qiu Y, Liu J, Yu W, Fu X, Zhu B, Xu X, Duan A, Duan A, et al: Bile cell‑free DNA as a novel and powerful liquid biopsy for detecting somatic variants in biliary tract cancer. Oncol Rep 42: 549-560, 2019.
APA
Shen, N., Zhang, D., Yin, L., Qiu, Y., Liu, J., Yu, W. ... Zhang, Y. (2019). Bile cell‑free DNA as a novel and powerful liquid biopsy for detecting somatic variants in biliary tract cancer. Oncology Reports, 42, 549-560. https://doi.org/10.3892/or.2019.7177
MLA
Shen, N., Zhang, D., Yin, L., Qiu, Y., Liu, J., Yu, W., Fu, X., Zhu, B., Xu, X., Duan, A., Chen, Z., Wang, X., Cao, X., Zhao, T., Zhou, Z., Yu, L., Qin, H., Fang, Z., Li, J., Liu, Y., Xiong, L., Yuan, B., Li, F., Zhang, Y."Bile cell‑free DNA as a novel and powerful liquid biopsy for detecting somatic variants in biliary tract cancer". Oncology Reports 42.2 (2019): 549-560.
Chicago
Shen, N., Zhang, D., Yin, L., Qiu, Y., Liu, J., Yu, W., Fu, X., Zhu, B., Xu, X., Duan, A., Chen, Z., Wang, X., Cao, X., Zhao, T., Zhou, Z., Yu, L., Qin, H., Fang, Z., Li, J., Liu, Y., Xiong, L., Yuan, B., Li, F., Zhang, Y."Bile cell‑free DNA as a novel and powerful liquid biopsy for detecting somatic variants in biliary tract cancer". Oncology Reports 42, no. 2 (2019): 549-560. https://doi.org/10.3892/or.2019.7177
Copy and paste a formatted citation
x
Spandidos Publications style
Shen N, Zhang D, Yin L, Qiu Y, Liu J, Yu W, Fu X, Zhu B, Xu X, Duan A, Duan A, et al: Bile cell‑free DNA as a novel and powerful liquid biopsy for detecting somatic variants in biliary tract cancer. Oncol Rep 42: 549-560, 2019.
APA
Shen, N., Zhang, D., Yin, L., Qiu, Y., Liu, J., Yu, W. ... Zhang, Y. (2019). Bile cell‑free DNA as a novel and powerful liquid biopsy for detecting somatic variants in biliary tract cancer. Oncology Reports, 42, 549-560. https://doi.org/10.3892/or.2019.7177
MLA
Shen, N., Zhang, D., Yin, L., Qiu, Y., Liu, J., Yu, W., Fu, X., Zhu, B., Xu, X., Duan, A., Chen, Z., Wang, X., Cao, X., Zhao, T., Zhou, Z., Yu, L., Qin, H., Fang, Z., Li, J., Liu, Y., Xiong, L., Yuan, B., Li, F., Zhang, Y."Bile cell‑free DNA as a novel and powerful liquid biopsy for detecting somatic variants in biliary tract cancer". Oncology Reports 42.2 (2019): 549-560.
Chicago
Shen, N., Zhang, D., Yin, L., Qiu, Y., Liu, J., Yu, W., Fu, X., Zhu, B., Xu, X., Duan, A., Chen, Z., Wang, X., Cao, X., Zhao, T., Zhou, Z., Yu, L., Qin, H., Fang, Z., Li, J., Liu, Y., Xiong, L., Yuan, B., Li, F., Zhang, Y."Bile cell‑free DNA as a novel and powerful liquid biopsy for detecting somatic variants in biliary tract cancer". Oncology Reports 42, no. 2 (2019): 549-560. https://doi.org/10.3892/or.2019.7177
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