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Article

EMX2 is epigenetically silenced and suppresses epithelial‑mesenchymal transition in human esophageal adenocarcinoma

  • Authors:
    • Lei Wang
    • Joy Jin
    • Yong Zhou
    • Ziqiang Tian
    • Biao He
    • Yueyan Huang
    • Feng Ding
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China, Thoracic Oncology Program, Department of Surgery, Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, San Francisco, CA 94115, USA, Department of Pharmacology, Medical College of Jiaxing University, Jiaxing, Zhejiang 314000, P.R. China, Department of Biotechnology and Biomedicine, Zhejiang Provincial Key Laboratory of Applied Enzymology and Jiaxing ACCB Diagnostics, Yangze Delta Region Institute of Tsinghua University Zhejiang, Jiaxing, Zhejiang 314006, P.R. China
  • Pages: 2169-2178
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    Published online on: August 20, 2019
       https://doi.org/10.3892/or.2019.7284
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Abstract

Esophageal adenocarcinoma (EAC) is an aggressive and challenging disease to treat, with an overall five‑year survival rate of <20%. Early malignant cell dissemination contributes to this poor prognosis. Epithelial‑mesenchymal transition (EMT) induces the invasion and metastasis of carcinoma cells. Empty spiracles homeobox 2 (EMX2) is a homeodomain‑containing transcription factor, which is associated with numerous cancer types, and has been demonstrated to regulate EMT. In the present study, 48 pairs of EAC and adjacent normal tissues were analyzed. The results revealed that EMX2 was downregulated in EAC tissues, and its expression was negatively correlated with the DNA hypermethylation of its promoter. Additionally, the OE19 and OE33 EAC cell lines were treated with the DNA methyltransferase inhibitor 5‑aza‑2'‑deoxycytidine, and the results indicated that EMX2 expression was increased. Overexpressing EMX2 in EAC cell lines enhanced the expression of apoptotic markers, inhibited cell migration and invasion, led to the upregulation of E‑cadherin and the downregulation of mesenchymal markers, and suppressed AKT, mTOR and S6K phosphorylation. Furthermore, EMX2 overexpression sensitized EAC cells to cisplatin. These results demonstrated that EMX2 inhibited the AKT/mTOR/S6K signaling pathway and decreased EMT. However, the downregulation of EMX2 was revealed to be associated with EMT in EAC, indicating that EMX2 may be a potential target for the management of EAC.
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Copy and paste a formatted citation
Spandidos Publications style
Wang L, Jin J, Zhou Y, Tian Z, He B, Huang Y and Ding F: EMX2 is epigenetically silenced and suppresses epithelial‑mesenchymal transition in human esophageal adenocarcinoma. Oncol Rep 42: 2169-2178, 2019.
APA
Wang, L., Jin, J., Zhou, Y., Tian, Z., He, B., Huang, Y., & Ding, F. (2019). EMX2 is epigenetically silenced and suppresses epithelial‑mesenchymal transition in human esophageal adenocarcinoma. Oncology Reports, 42, 2169-2178. https://doi.org/10.3892/or.2019.7284
MLA
Wang, L., Jin, J., Zhou, Y., Tian, Z., He, B., Huang, Y., Ding, F."EMX2 is epigenetically silenced and suppresses epithelial‑mesenchymal transition in human esophageal adenocarcinoma". Oncology Reports 42.5 (2019): 2169-2178.
Chicago
Wang, L., Jin, J., Zhou, Y., Tian, Z., He, B., Huang, Y., Ding, F."EMX2 is epigenetically silenced and suppresses epithelial‑mesenchymal transition in human esophageal adenocarcinoma". Oncology Reports 42, no. 5 (2019): 2169-2178. https://doi.org/10.3892/or.2019.7284
Copy and paste a formatted citation
x
Spandidos Publications style
Wang L, Jin J, Zhou Y, Tian Z, He B, Huang Y and Ding F: EMX2 is epigenetically silenced and suppresses epithelial‑mesenchymal transition in human esophageal adenocarcinoma. Oncol Rep 42: 2169-2178, 2019.
APA
Wang, L., Jin, J., Zhou, Y., Tian, Z., He, B., Huang, Y., & Ding, F. (2019). EMX2 is epigenetically silenced and suppresses epithelial‑mesenchymal transition in human esophageal adenocarcinoma. Oncology Reports, 42, 2169-2178. https://doi.org/10.3892/or.2019.7284
MLA
Wang, L., Jin, J., Zhou, Y., Tian, Z., He, B., Huang, Y., Ding, F."EMX2 is epigenetically silenced and suppresses epithelial‑mesenchymal transition in human esophageal adenocarcinoma". Oncology Reports 42.5 (2019): 2169-2178.
Chicago
Wang, L., Jin, J., Zhou, Y., Tian, Z., He, B., Huang, Y., Ding, F."EMX2 is epigenetically silenced and suppresses epithelial‑mesenchymal transition in human esophageal adenocarcinoma". Oncology Reports 42, no. 5 (2019): 2169-2178. https://doi.org/10.3892/or.2019.7284
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