Open Access

Tanshinone IIA inhibits glucose metabolism leading to apoptosis in cervical cancer

  • Authors:
    • Zhigang Liu
    • Wenhe Zhu
    • Xiangyu Kong
    • Xi Chen
    • Xinyi Sun
    • Wei Zhang
    • Ruowen Zhang
  • View Affiliations

  • Published online on: August 27, 2019     https://doi.org/10.3892/or.2019.7294
  • Pages: 1893-1903
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Cancer requires aerobic glycolysis to supply the energy required for proliferation. Existing evidence has revealed that blocking glycolysis results in apoptosis of cancer cells. Tanshinone IIA (Tan IIA) is a diterpenoid naphthoquinone found in traditional Chinese medicine, Danshen (Salvia sp.). Tan IIA exhibits potential anticancer activity. However, its effect on cell viability of human cervical cancer cells and its mechanism are unknown. The aim of the present study was to determine the effect of Tan IIA on proliferation and glucose metabolism in cervical cancer cells. Cell viability was measured by MTT assay, apoptosis was determined using flow cytometry and glucose uptake, lactate production, and adenosine triphosphate content were measured to assess glucose metabolism. The expression of apoptosis‑associated proteins was detected by western blotting and the antitumor activity of Tan IIA in vivo was evaluated in cervical carcinoma‑bearing mice. The results revealed Tan IIA treatment resulted in a considerable reduction in the viability of SiHa cells. Tan IIA decreased the expression of HPV oncogenes E6 and E7, induced apoptosis and also decreased glycolysis by suppressing the activity of the intracellular AKT/mTOR and HIF‑1α. In vivo, treatment with Tan IIA resulted in a 72.7% reduction in tumor volume. The present study highlights the potential therapeutic value of Tan IIA, which functions by inducing apoptosis and may be associated with inhibition of glycolysis.
View Figures
View References

Related Articles

Journal Cover

November-2019
Volume 42 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Liu Z, Zhu W, Kong X, Chen X, Sun X, Zhang W and Zhang R: Tanshinone IIA inhibits glucose metabolism leading to apoptosis in cervical cancer. Oncol Rep 42: 1893-1903, 2019
APA
Liu, Z., Zhu, W., Kong, X., Chen, X., Sun, X., Zhang, W., & Zhang, R. (2019). Tanshinone IIA inhibits glucose metabolism leading to apoptosis in cervical cancer. Oncology Reports, 42, 1893-1903. https://doi.org/10.3892/or.2019.7294
MLA
Liu, Z., Zhu, W., Kong, X., Chen, X., Sun, X., Zhang, W., Zhang, R."Tanshinone IIA inhibits glucose metabolism leading to apoptosis in cervical cancer". Oncology Reports 42.5 (2019): 1893-1903.
Chicago
Liu, Z., Zhu, W., Kong, X., Chen, X., Sun, X., Zhang, W., Zhang, R."Tanshinone IIA inhibits glucose metabolism leading to apoptosis in cervical cancer". Oncology Reports 42, no. 5 (2019): 1893-1903. https://doi.org/10.3892/or.2019.7294