Open Access

Acquisition of temozolomide resistance by the rat C6 glioma cell line increases cell migration and side population phenotype

  • Authors:
    • Ya Xu
    • Xiangcai Yang
    • Shuting Mei
    • Yi Sun
    • Jiejing Li
  • View Affiliations

  • Published online on: October 1, 2019     https://doi.org/10.3892/or.2019.7350
  • Pages: 2355-2362
  • Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cancer stem cells are reportedly associated with drug resistance in glioma, but there are conflicting findings on the effects of cancer stem cells on drug resistance. The aim of the present study was to identify the underlying mechanisms of drug resistance in rat C6 glioma cells, through the use of Transwell invasion assays, flow cytometric and western blot analyses as well as immunohistochemical staining. The results revealed that acquisition of drug resistance by C6 cells enhanced migration ability in vivo and in vitro. Notably, drug resistance did not depend on the cancer stem cells of C6 cells, but on the increase of a side population phenotype. Blockade of the ABC transporter could increase sensitivity to temozolomide and temozolomide‑induced apoptosis in C6 cells. Collectively, these data indicated that drug resistance of C6 cells was mediated by the side population phenotype rather than by cancer stem cells.
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December-2019
Volume 42 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Xu Y, Yang X, Mei S, Sun Y and Li J: Acquisition of temozolomide resistance by the rat C6 glioma cell line increases cell migration and side population phenotype. Oncol Rep 42: 2355-2362, 2019
APA
Xu, Y., Yang, X., Mei, S., Sun, Y., & Li, J. (2019). Acquisition of temozolomide resistance by the rat C6 glioma cell line increases cell migration and side population phenotype. Oncology Reports, 42, 2355-2362. https://doi.org/10.3892/or.2019.7350
MLA
Xu, Y., Yang, X., Mei, S., Sun, Y., Li, J."Acquisition of temozolomide resistance by the rat C6 glioma cell line increases cell migration and side population phenotype". Oncology Reports 42.6 (2019): 2355-2362.
Chicago
Xu, Y., Yang, X., Mei, S., Sun, Y., Li, J."Acquisition of temozolomide resistance by the rat C6 glioma cell line increases cell migration and side population phenotype". Oncology Reports 42, no. 6 (2019): 2355-2362. https://doi.org/10.3892/or.2019.7350