Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1021-335X Online ISSN: 1791-2431
Journal Cover
April-2020 Volume 43 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
April-2020 Volume 43 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML

  • Supplementary Files
    • Supplementary_Data.pdf
Article Open Access

Epigenetic control of the EWS‑FLI1 promoter in Ewing's sarcoma

  • Authors:
    • C.  Montoya
    • L. Rey
    • J. Rodríguez
    • M. J. Fernández
    • D.  Troncoso
    • A.  Cañas
    • O. Moreno
    • B. Henríquez
    • A. Rojas
  • View Affiliations / Copyright

    Affiliations: Institute of Human Genetics, Faculty of Medicine, Pontificia Universidad Javeriana, Bogotá 110231, Colombia, Chile, Department of Pathology, Hospital Universitario San Ignacio, Bogotá 110231, Colombia, Chile, Department of Physiological Sciences, Faculty of Medicine, Pontificia Universidad Javeriana, Bogotá 110231, Colombia, Chile, Department of Internal Medicine, Hospital Universitario San Ignacio, Bogotá 110231, Colombia, Chile, Institute of Human Genetics, Faculty of Medicine, Pontificia Universidad Javeriana, Bogotá 110231, Colombia, Faculty of Medicine and Science, Universidad San Sebastián, Santiago 7510157, Chile
    Copyright: © Montoya et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1199-1207
    |
    Published online on: February 5, 2020
       https://doi.org/10.3892/or.2020.7489
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Ewing sarcoma (ES) is a primary bone marrow tumor that very rarely develops in extra‑osseous tissues, such as lung. The hallmark of ES tumors is a translocation between chromosomes 11 and 22, resulting in a fusion protein, commonly referred to as EWS‑FLI1. The epigenetic profile (histone acetylation and methylation enrichment of the promoter region) that may regulate the expression of the aberrant transcription factor EWS‑FLI1, remains poorly studied and understood. Knowledge of epigenetic patterns associated with covalent histone modification and expression of enzymes associated with this process, can contribute to the understanding of the molecular basis of the disease, as well as to the identification of possible molecular targets involved in expression of the EWS‑FLI1 gene, so that therapeutic strategies may be improved in the future. In the present study, the transcriptional activation and repression of the EWS‑FLI1 fusion gene in ES was accompanied by selective deposition of histone markers on its promoter. The EWS‑FLI1 fusion gene was evaluated in two patients with ES using conventional cytogenetic, fluorescence in situ hybridization and nested PCR assays, which revealed that the aberrant expression of the EWS‑FLI1 gene is accompanied by enrichment of H3K4Me3, H3K9ac and H3K27ac at the promoter region.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Desai KI, Nadkarni TD, Goel A, Muzumdar DP, Naresh KN and Nair CN: Primary Ewing's sarcoma of the cranium. Neurosurgery. 46:62–68. 2000. View Article : Google Scholar : PubMed/NCBI

2 

Deokar K, Nana GK and Shivhari G: Primary ewings sarcoma of the lung. J Clin Diagn Res. 9:XD01–XD03. 2015.PubMed/NCBI

3 

de Alava E: Patología molecular de los srcomas. Oncología (Barcelona). 28:22–38. 2005.(In Spain). View Article : Google Scholar

4 

Kumar N and Gupta B: Global incidence of primary malignant bone tumors. Curr Orthopaedic Practice. 27:52016. View Article : Google Scholar

5 

Soto C, Gómez L, Criollo F, Romo R, Messa Ó and Arbeláez P: Sarcoma de Ewing de la falange proximal del meñique. Reporte de caso. Revista Colombiana de Cancerología. 18:137–142. 2014.(In Spain). View Article : Google Scholar

6 

Dynamed, . Ewing sarcoma in children. Available at:. https://www.dynamed.com/condition/ewing-sarcoma-in-children

7 

Desai SS and Jambhekar NA: Pathology of Ewing's sarcoma/PNET: Current opinion and emerging concepts. Indian J Orthop. 44:363–368. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Kaneko Y, Kobayashi H, Handa M, Satake N and Maseki N: EWS-ERG fusion transcript produced by chromosomal insertion in a Ewing sarcoma. Genes Chromosomes Cancer. 18:228–231. 1997. View Article : Google Scholar : PubMed/NCBI

9 

Kim SK and Park YK: Ewing sarcoma: A chronicle of molecular pathogenesis. Hum Pathol. 55:91–100. 2016. View Article : Google Scholar : PubMed/NCBI

10 

Balamuth NJ and Womer RB: Ewing's sarcoma. Lancet Oncol. 11:184–192. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Franzetti GA, Laud-Duval K, Bellanger D, Stern MH, Sastre-Garau X and Delattre O: MiR-30a-5p connects EWS-FLI1 and CD99, two major therapeutic targets in Ewing tumor. Oncogene. 32:3915–3921. 2012. View Article : Google Scholar

12 

Matsumoto Y, Tanaka K, Nakatani F, Matsunobu T, Matsuda S and Iwamoto Y: Downregulation and forced expression of EWS-Fli1 fusion gene results in changes in the expression of G(1)regulatory genes. Br J Cancer. 84:768–775. 2001. View Article : Google Scholar : PubMed/NCBI

13 

Randall L, Calvert G, Spraker H, Lessnick S and Sarcoma de Ewing: Diagnóstico, tratamiento y pronóstico. Liddy Shriver Sarcoma Initiative. Available at:. http://sarcomahelp.org/translate/es-sarcoma-ewing.htmlJan 18–2018

14 

Lawlor ER and Thiele CJ: Epigenetic changes in pediatric solid tumors: Promising new targets. Clin Cancer Res. 18:2768–2779. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Cavalheiro dos Santos D, da Cruz Evangelista L and Kerche-Silva L: Genetic alterations and diagnosis in Ewing sarcoma: A review. Oatext.com (2017). Available at:. http://www.oatext.com/genetic-alterations-and-diagnosis-in-ewing-sarcoma-a-review.phpApr 5–2018

16 

Parrish JK, Sechler M, Winn RA and Jedlicka P: The histone demethylase KDM3A is a microRNA-22-regulated tumor promoter in Ewing sarcoma. Oncogene. 34:257–262. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Rocchi A, Manara MC, Sciandra M, Zambelli D, Nardi F, Nicoletti G, Garofalo C, Meschini S, Astolfi A, Colombo MP, et al: CD99 inhibits neural differentiation of human Ewing sarcoma cells and thereby contributes to oncogenesis. J Clin Invest. 120:668–680. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Herreño AM, Fernández MJ, Rey L, Mejia JA, Cañas A, Moreno OM, Henríquez B, Montecino MA and Rojas AP: Primary lung cancer cell culture from transthoracic needle biopsy samples. Cogent Med. 5:12018. View Article : Google Scholar :

19 

Vural C, Uluoğlu O, Akyürek N, Oğuz A and Karadeniz C: The evaluation of CD99 immunoreactivity and EWS/FLI1 translocation by fluorescence in situ hybridization in central PNETs and Ewing's sarcoma family of tumors. Pathol Oncol Res. 17:619–625. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Moorhead PS, Nowell PC, Mellman WJ, Battips DM and Hungerford DA: Chromosome preparations of leukocytes cultured from human peripheral blood. Exp Cell Res. 20:613–616. 1960. View Article : Google Scholar : PubMed/NCBI

21 

McGowan-Jordan J, Simons A and Schmid M: ISCN 2016. An International System for Human Cytogenomic Nomenclature (2016). Karger; Basel: 2016, View Article : Google Scholar

22 

Elzi DJ, Song M, Houghton PJ, Chen Y and Shiio Y: The role of FLI-1-EWS, a fusion gene reciprocal to EWS-FLI-1, in Ewing sarcoma. Genes Cancer. 6:452–461. 2015.PubMed/NCBI

23 

Przybyl J, Kozak K, Kosela H, Falkowski S, Switaj T, Lugowska I, Szumera-Cieckiewicz A, Ptaszynski K, Grygalewicz B, Chechlinska M, et al: Gene expression profiling of peripheral blood cells: New insights into Ewing sarcoma biology and clinical applications. Med Oncol. 31:1092014. View Article : Google Scholar : PubMed/NCBI

24 

Abcam (2011). A-beginners-guide-to-ChIP. Available at. http://docs.abcam.com/pdf/chromatin/A-beginners-guide-to-ChIP.pdfJan 22–2018

25 

Jacques C, Lamoureux F, Baud'huin M, Rodriguez Calleja L, Quillard T, Amiaud J, Tirode F, Rédini F, Bradner JE, Heymann D and Ory B: Targeting the epigenetic readers in Ewing Sarcoma inhibits the oncogenic transcription factor EWS/Fli1. Oncotarget. 7:24125–24140. 2016. View Article : Google Scholar : PubMed/NCBI

26 

Haring M, Offermann S, Danker T, Horst I, Peterhansel C and Stam M: Chromatin immunoprecipitation: Optimization, quantitative analysis and data normalization. Plant Methods. 3:112007. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Montoya C, Rey L, Rodríguez J, Fernández MJ, Troncoso D, Cañas A, Moreno O, Henríquez B and Rojas A: Epigenetic control of the EWS‑FLI1 promoter in Ewing's sarcoma. Oncol Rep 43: 1199-1207, 2020.
APA
Montoya, C., Rey, L., Rodríguez, J., Fernández, M.J., Troncoso, D., Cañas, A. ... Rojas, A. (2020). Epigenetic control of the EWS‑FLI1 promoter in Ewing's sarcoma. Oncology Reports, 43, 1199-1207. https://doi.org/10.3892/or.2020.7489
MLA
Montoya, C., Rey, L., Rodríguez, J., Fernández, M. J., Troncoso, D., Cañas, A., Moreno, O., Henríquez, B., Rojas, A."Epigenetic control of the EWS‑FLI1 promoter in Ewing's sarcoma". Oncology Reports 43.4 (2020): 1199-1207.
Chicago
Montoya, C., Rey, L., Rodríguez, J., Fernández, M. J., Troncoso, D., Cañas, A., Moreno, O., Henríquez, B., Rojas, A."Epigenetic control of the EWS‑FLI1 promoter in Ewing's sarcoma". Oncology Reports 43, no. 4 (2020): 1199-1207. https://doi.org/10.3892/or.2020.7489
Copy and paste a formatted citation
x
Spandidos Publications style
Montoya C, Rey L, Rodríguez J, Fernández MJ, Troncoso D, Cañas A, Moreno O, Henríquez B and Rojas A: Epigenetic control of the EWS‑FLI1 promoter in Ewing's sarcoma. Oncol Rep 43: 1199-1207, 2020.
APA
Montoya, C., Rey, L., Rodríguez, J., Fernández, M.J., Troncoso, D., Cañas, A. ... Rojas, A. (2020). Epigenetic control of the EWS‑FLI1 promoter in Ewing's sarcoma. Oncology Reports, 43, 1199-1207. https://doi.org/10.3892/or.2020.7489
MLA
Montoya, C., Rey, L., Rodríguez, J., Fernández, M. J., Troncoso, D., Cañas, A., Moreno, O., Henríquez, B., Rojas, A."Epigenetic control of the EWS‑FLI1 promoter in Ewing's sarcoma". Oncology Reports 43.4 (2020): 1199-1207.
Chicago
Montoya, C., Rey, L., Rodríguez, J., Fernández, M. J., Troncoso, D., Cañas, A., Moreno, O., Henríquez, B., Rojas, A."Epigenetic control of the EWS‑FLI1 promoter in Ewing's sarcoma". Oncology Reports 43, no. 4 (2020): 1199-1207. https://doi.org/10.3892/or.2020.7489
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team