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Long non‑coding RNA H19 is involved in sorafenib resistance in hepatocellular carcinoma by upregulating miR‑675

  • Authors:
    • Yongzi Xu
    • Yanhui Liu
    • Zhenrong Li
    • Hao Li
    • Xiqing Li
    • Lei Yan
    • Jiayan Mao
    • Jian Shen
    • Wei Chen
    • Fei Xue
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, Nanjing, Jiangsu 210009, P.R. China, Department of Hematology, Henan Provincial People's Hospital, Zhengzhou, Henan 450003, P.R. China, Department of Hepatobiliary and Pancreatic Surgery, Henan Provincial People's Hospital, Zhengzhou, Henan 450003, P.R. China, Department of Oncology, Henan Provincial People's Hospital, Zhengzhou, Henan 450003, P.R. China, Department of Nephrology, Henan Provincial People's Hospital, Zhengzhou, Henan 450003, P.R. China, Cancer Institute of Integrated Traditional Chinese and Western Medicine, Key Laboratory of Cancer Prevention and Therapy Combining Traditional Chinese and Western Medicine, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou, Zhejiang 310012, P.R. China
    Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 165-173
    |
    Published online on: May 8, 2020
       https://doi.org/10.3892/or.2020.7608
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Abstract

Sorafenib is the first‑line treatment for advanced hepatocellular carcinoma (HCC). Since many HCC patients experience drug resistance, there is an urgent need to discover more effective therapeutic strategies to overcome drug resistance. Long non‑coding RNAs (lncRNAs) play an important role in tumor drug resistance. However, research on the role of lncRNA H19 in sorafenib resistance in HCC is quite limited. In the present study, CCK‑8 assay, RT‑qPCR, EdU staining, immunofluorescence staining, and western blot analysis were used to detect the effect of lncRNA H19 on sorafenib resistance of HCC cells. H19 expression was found to be negatively related to sorafenib sensitivity in HCC cells. Knockdown of lncRNA H19 elevated sorafenib sensitivity by suppressing epithelial‑mesenchymal transition (EMT) in HCC cells. H19 upregulated miR‑675 expression. miR‑675 inhibitor decreased the cell viability in sorafenib‑treated HCC cells, while miR‑675 overexpression had the opposite effect on the treated cells. When the cells were pretreated with miR‑675 mimic, H19 siRNA did not alter the effect of miR‑675 on sorafenib sensitivity. In conclusion, our study provides new clues for further clinical treatment of sorafenib‑resistant liver cancer patients.
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Copy and paste a formatted citation
Spandidos Publications style
Xu Y, Liu Y, Li Z, Li H, Li X, Yan L, Mao J, Shen J, Chen W, Xue F, Xue F, et al: Long non‑coding RNA H19 is involved in sorafenib resistance in hepatocellular carcinoma by upregulating miR‑675. Oncol Rep 44: 165-173, 2020.
APA
Xu, Y., Liu, Y., Li, Z., Li, H., Li, X., Yan, L. ... Xue, F. (2020). Long non‑coding RNA H19 is involved in sorafenib resistance in hepatocellular carcinoma by upregulating miR‑675. Oncology Reports, 44, 165-173. https://doi.org/10.3892/or.2020.7608
MLA
Xu, Y., Liu, Y., Li, Z., Li, H., Li, X., Yan, L., Mao, J., Shen, J., Chen, W., Xue, F."Long non‑coding RNA H19 is involved in sorafenib resistance in hepatocellular carcinoma by upregulating miR‑675". Oncology Reports 44.1 (2020): 165-173.
Chicago
Xu, Y., Liu, Y., Li, Z., Li, H., Li, X., Yan, L., Mao, J., Shen, J., Chen, W., Xue, F."Long non‑coding RNA H19 is involved in sorafenib resistance in hepatocellular carcinoma by upregulating miR‑675". Oncology Reports 44, no. 1 (2020): 165-173. https://doi.org/10.3892/or.2020.7608
Copy and paste a formatted citation
x
Spandidos Publications style
Xu Y, Liu Y, Li Z, Li H, Li X, Yan L, Mao J, Shen J, Chen W, Xue F, Xue F, et al: Long non‑coding RNA H19 is involved in sorafenib resistance in hepatocellular carcinoma by upregulating miR‑675. Oncol Rep 44: 165-173, 2020.
APA
Xu, Y., Liu, Y., Li, Z., Li, H., Li, X., Yan, L. ... Xue, F. (2020). Long non‑coding RNA H19 is involved in sorafenib resistance in hepatocellular carcinoma by upregulating miR‑675. Oncology Reports, 44, 165-173. https://doi.org/10.3892/or.2020.7608
MLA
Xu, Y., Liu, Y., Li, Z., Li, H., Li, X., Yan, L., Mao, J., Shen, J., Chen, W., Xue, F."Long non‑coding RNA H19 is involved in sorafenib resistance in hepatocellular carcinoma by upregulating miR‑675". Oncology Reports 44.1 (2020): 165-173.
Chicago
Xu, Y., Liu, Y., Li, Z., Li, H., Li, X., Yan, L., Mao, J., Shen, J., Chen, W., Xue, F."Long non‑coding RNA H19 is involved in sorafenib resistance in hepatocellular carcinoma by upregulating miR‑675". Oncology Reports 44, no. 1 (2020): 165-173. https://doi.org/10.3892/or.2020.7608
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