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Article

Long non‑coding RNA FER1L4 inhibits cell proliferation and promotes cell apoptosis via the PTEN/AKT/p53 signaling pathway in lung cancer

  • Authors:
    • Lanwei Ouyang
    • Min Yang
    • Xiaolin Wang
    • Jidan Fan
    • Xing Liu
    • Yong Zhang
    • Yusheng Shu
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, Northern Jiangsu People's Hospital, Yangzhou, Jiangsu 225001, P.R. China, Department of Cardiac Macrovascular Center, Northern Jiangsu People's Hospital, Yangzhou, Jiangsu 225001, P.R. China, Department of Surgical Oncology, The Second People's Hospital of Yichang, Yichang, Hubei 443000, P.R. China
  • Pages: 359-367
    |
    Published online on: November 19, 2020
       https://doi.org/10.3892/or.2020.7861
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Abstract

Long non‑coding RNA Fer‑1‑like protein 4 (FER1L4) has been reported to play crucial regulatory roles in tumor progression and apoptosis. However, its clinical significance and biological role in non‑small cell lung cancer (NSCLC) are completely unknown. The purpose of this study was to investigate the expression of lncRNA FER1L4 in plasma and tissues of patients with NSCLC and study the mechanism of proliferation and apoptosis of lung cancer cells. The expression levels of FER1L4 in plasma and tissues of NSCLC patients and cell lines were analyzed via RT‑qPCR. The effects of FER1L4 on cell proliferation, migration and invasion were analyzed by CCK‑8, wound healing and Transwell assays, respectively. The expression levels of related proteins were detected by western blot assay, while cell apoptosis was determined by Hoechst staining and flow cytometry. The results revealed that FER1L4 was significantly downregulated in NSCLC plasma and tissues and lung cancer cell lines compared to corresponding controls. Moreover, a significant decrease of cell proliferation, migration and invasion were observed in FER1L4‑overexpressed cells. FER1L4 could promote phosphatase and tension homolog deleted on chromosome ten (PTEN) and p53 expression, inhibit AKT phosphorylation expression, thus increasing the proportion of apoptotic cells. The present study indicated that FER1L4 may inhibit cell proliferation and promote apoptosis of NSCLC cells via the PTEN/AKT/p53 pathway, which provides a better understanding of the pathogenesis of NSCLC and may provide a novel potential therapeutic target for clinical treatment.
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1 

Mao Y, Yang D, He J and Krasna MJ: Epidemiology of lung cancer. Surg Oncol Clin N Am. 25:439–445. 2016. View Article : Google Scholar

2 

Zheng RS, Sun KX, Zhang SW, Zeng HM, Zou XN, Chen R, Gu XY, Wei WW and He J: Report of cancer epidemiology in China, 2015. Zhonghua Zhong Liu Za Zhi. 41:19–28. 2019.(In Chinese).

3 

Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA and Jemal A: Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 68:394–424. 2018. View Article : Google Scholar

4 

Hirsch FR, Scagliotti GV, Mulshine JL, Kwon R, Curran WJ Jr, Wu YL and Ares LP: Lung cancer: Current therapies and new targeted treatments. Lancet. 389:299–311. 2017. View Article : Google Scholar

5 

Bhan A, Soleimani M and Mandal SS: Long noncoding RNA and cancer: A new paradigm. Cancer Res. 77:3965–3981. 2017. View Article : Google Scholar

6 

Xia T, Chen S, Jiang Z, Shao Y, Jiang X, Li P, Xiao B and Guo J: Long noncoding RNA FER1L4 suppresses cancer cell growth by acting as a competing endogenous RNA and regulating PTEN expression. Sci Rep. 5:134452015. View Article : Google Scholar

7 

Meng Q, Ren M, Li Y and Song X: LncRNA-RMRP acts as an oncogene in lung cancer. PLoS One. 11:e01648452016. View Article : Google Scholar

8 

Guo F, Cao Z, Guo H and Li S: The action mechanism of lncRNA-HOTAIR on the drug resistance of non-small cell lung cancer by regulating wnt signaling pathway. Exp Ther Med. 15:4885–4889. 2018.

9 

Wang F, Chen JG, Wang LL, Yan ZZ, Chen SP and Wang XG: Up-Regulation of LINC00346 inhibits proliferation of non-small cell lung cancer cells through mediating JAK-STAT3 signaling pathway. Eur Rev Med Pharmacol Sci. 21:5135–5142. 2017.

10 

Zhang E, Li W, Yin D, De W, Zhu L, Sun S and Han L: C-Myc-Regulated long non-coding RNA H19 indicates a poor prognosis and affects cell proliferation in non-small-cell lung cancer. Tumour Biol. 37:4007–4015. 2016. View Article : Google Scholar

11 

Wu J, Huang J, Wang W, Xu J, Yin M, Cheng N and Yin J: Long non-coding RNA fer-1-like protein 4 acts as a tumor suppressor via miR-106a-5p and predicts good prognosis in hepatocellular carcinoma. Cancer Biomark. 20:55–65. 2017. View Article : Google Scholar

12 

Wang X, Dong K, Jin Q, Ma Y, Yin S and Wang S: Upregulation of lncRNA FER1L4 suppresses the proliferation and migration of the hepatocellular carcinoma via regulating PI3K/AKT signal pathway. J Cell Biochem. 120:6781–6788. 2019. View Article : Google Scholar

13 

Yue B, Sun B, Liu C, Zhao S, Zhang D, Yu F and Yan D: Long non-coding RNA fer-1-like protein 4 suppresses oncogenesis and exhibits prognostic value by associating with miR-106a-5p in colon cancer. Cancer Sci. 106:1323–1332. 2015. View Article : Google Scholar

14 

Liu Z, Shao Y, Tan L, Shi H, Chen S and Guo J: Clinical significance of the low expression of FER1L4 in gastric cancer patients. Tumour Biol. 35:9613–9617. 2014. View Article : Google Scholar

15 

Fei D, Zhang X, Liu J, Tan L, Xing J, Zhao D and Zhang Y: Long noncoding RNA FER1L4 suppresses tumorigenesis by regulating the expression of PTEN targeting miR-18a-5p in osteosarcoma. Cell Physiol Biochem. 51:1364–1375. 2018. View Article : Google Scholar

16 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar

17 

Wu J, Li L, Wang Y, Ren X, Lin K and He Y: The HSP90/AKT pathway may mediate artemether-induced apoptosis of Cal27 cells. FEBS Open Bio. 9:1726–1733. 2019. View Article : Google Scholar

18 

Zhang LX, Ding F, Wang CQ, Bing Q, Zhao Z, Wang J and Zhang L: MiR-181a affects myocardial ischemia-reperfusion injury in rats via regulating akt signaling pathway. Eur Rev Med Pharmacol Sci. 23:6292–6298. 2019.

19 

Travis WD, Brambilla E, Nicholson AG, Yatabe Y, Austin JHM, Beasley MB, Chirieac LR, Dacic S, Duhig E, Flieder DB, et al: The 2015 world health organization classification of lung tumors: Impact of genetic, clinical and radiologic advances since the 2004 classification. J Thorac Oncol. 10:1243–1260. 2015. View Article : Google Scholar

20 

Li Y, Wang Z, Shi H, Li H, Li L, Fang R, Cai X, Liu B, Zhang X and Ye L: HBXIP and LSD1 scaffolded by lncrna hotair mediate transcriptional activation by c-myc. Cancer Res. 76:293–304. 2016. View Article : Google Scholar

21 

Qi F, Liu X, Wu H, Yu X, Wei C, Huang X, Ji G, Nie F and Wang K: Long noncoding AGAP2-AS1 is activated by SP1 and promotes cell proliferation and invasion in gastric cancer. J Hematol Oncol. 10:482017. View Article : Google Scholar

22 

Iyer MK, Niknafs YS, Malik R, Singhal U, Sahu A, Hosono Y, Barrette TR, Prensner JR, Evans JR, Zhao S, et al: The landscape of long noncoding RNAs in the human transcriptome. Nat Genet. 47:199–208. 2015. View Article : Google Scholar

23 

Pan C, Yao G, Liu B, Ma T, Xia Y, Wei K, Wang J, Xu J, Chen L and Chen Y: Long noncoding RNA FAL1 promotes cell proliferation, invasion and epithelial-mesenchymal transition through the PTEN/AKT signaling axis in non-small cell lung cancer. Cell Physiol Biochem. 43:339–352. 2017. View Article : Google Scholar

24 

Lu Q, Shan S, Li Y, Zhu D, Jin W and Ren T: Long noncoding RNA SNHG1 promotes non-small cell lung cancer progression by up-regulating MTDH via sponging miR-145-5p. FASEB J. 32:3957–3967. 2018. View Article : Google Scholar

25 

Xia L, Nie D, Wang G, Sun C and Chen G: FER1L4/miR-372/E2F1 works as a ceRNA system to regulate the proliferation and cell cycle of glioma cells. J Cell Mol Med. 23:3224–3233. 2019. View Article : Google Scholar

26 

Qiao Q and Li H: LncRNA FER1L4 suppresses cancer cell proliferation and cycle by regulating PTEN expression in endometrial carcinoma. Biochem Biophys Res Commun. 478:507–512. 2016. View Article : Google Scholar

27 

Ma L, Zhang L, Guo A, Liu LC, Yu F, Diao N, Xu C and Wang D: Overexpression of FER1L4 promotes the apoptosis and suppresses epithelial-mesenchymal transition and stemness markers via activating PI3K/AKT signaling pathway in osteosarcoma cells. Pathol Res Pract. 215:1524122019. View Article : Google Scholar

28 

Sun X, Zheng G, Li C and Liu C: Long noncoding RNA fer1like family member 4 suppresses hepatocellular carcinoma cell proliferation by regulating PTEN in vitro and in vivo. Mol Med Rep. 19:685–692. 2019.

29 

Wang Z and Li C, Li Y, Guo X, Yan Z, Gao F and Li C: DpdtbA-Induced growth inhibition in human esophageal cancer cells involved inactivation of the p53/EGFR/AKT pathway. Oxid Med Cell Longev. 2019:54146702019. View Article : Google Scholar

30 

Han Z, Chen F, Ge X, Tan J, Lei P and Zhang J: MiR-21 alleviated apoptosis of cortical neurons through promoting PTEN-Akt signaling pathway in vitro after experimental traumatic brain injury. Brain Res. 1582:12–20. 2014. View Article : Google Scholar

31 

Chen J: The cell-cycle arrest and apoptotic functions of p53 in tumor initiation and progression. Cold Spring Harb Perspect Med. 6:a0261042016. View Article : Google Scholar

32 

Gao X, Wang N, Wu S, Cui H, An X and Yang Y: Long noncoding RNA FER1L4 inhibits cell proliferation and metastasis through regulation of the PI3K/AKT signaling pathway in lung cancer cells. Mol Med Rep. 20:182–190. 2019.

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Copy and paste a formatted citation
Spandidos Publications style
Ouyang L, Yang M, Wang X, Fan J, Liu X, Zhang Y and Shu Y: Long non‑coding RNA FER1L4 inhibits cell proliferation and promotes cell apoptosis via the PTEN/AKT/p53 signaling pathway in lung cancer. Oncol Rep 45: 359-367, 2021.
APA
Ouyang, L., Yang, M., Wang, X., Fan, J., Liu, X., Zhang, Y., & Shu, Y. (2021). Long non‑coding RNA FER1L4 inhibits cell proliferation and promotes cell apoptosis via the PTEN/AKT/p53 signaling pathway in lung cancer. Oncology Reports, 45, 359-367. https://doi.org/10.3892/or.2020.7861
MLA
Ouyang, L., Yang, M., Wang, X., Fan, J., Liu, X., Zhang, Y., Shu, Y."Long non‑coding RNA FER1L4 inhibits cell proliferation and promotes cell apoptosis via the PTEN/AKT/p53 signaling pathway in lung cancer". Oncology Reports 45.1 (2021): 359-367.
Chicago
Ouyang, L., Yang, M., Wang, X., Fan, J., Liu, X., Zhang, Y., Shu, Y."Long non‑coding RNA FER1L4 inhibits cell proliferation and promotes cell apoptosis via the PTEN/AKT/p53 signaling pathway in lung cancer". Oncology Reports 45, no. 1 (2021): 359-367. https://doi.org/10.3892/or.2020.7861
Copy and paste a formatted citation
x
Spandidos Publications style
Ouyang L, Yang M, Wang X, Fan J, Liu X, Zhang Y and Shu Y: Long non‑coding RNA FER1L4 inhibits cell proliferation and promotes cell apoptosis via the PTEN/AKT/p53 signaling pathway in lung cancer. Oncol Rep 45: 359-367, 2021.
APA
Ouyang, L., Yang, M., Wang, X., Fan, J., Liu, X., Zhang, Y., & Shu, Y. (2021). Long non‑coding RNA FER1L4 inhibits cell proliferation and promotes cell apoptosis via the PTEN/AKT/p53 signaling pathway in lung cancer. Oncology Reports, 45, 359-367. https://doi.org/10.3892/or.2020.7861
MLA
Ouyang, L., Yang, M., Wang, X., Fan, J., Liu, X., Zhang, Y., Shu, Y."Long non‑coding RNA FER1L4 inhibits cell proliferation and promotes cell apoptosis via the PTEN/AKT/p53 signaling pathway in lung cancer". Oncology Reports 45.1 (2021): 359-367.
Chicago
Ouyang, L., Yang, M., Wang, X., Fan, J., Liu, X., Zhang, Y., Shu, Y."Long non‑coding RNA FER1L4 inhibits cell proliferation and promotes cell apoptosis via the PTEN/AKT/p53 signaling pathway in lung cancer". Oncology Reports 45, no. 1 (2021): 359-367. https://doi.org/10.3892/or.2020.7861
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