Open Access

Erb‑B2 Receptor Tyrosine Kinase 2 is negatively regulated by the p53‑responsive microRNA‑3184‑5p in cervical cancer cells

  • Authors:
    • Hongli Liu
    • Yuzhi Li
    • Jing Zhang
    • Nan Wu
    • Fei Liu
    • Lihua Wang
    • Yuan Zhang
    • Jing Liu
    • Xuan Zhang
    • Suyang Guo
    • Hongtao Wang
  • View Affiliations

  • Published online on: November 19, 2020     https://doi.org/10.3892/or.2020.7862
  • Pages: 95-106
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The oncogenic role of Erb‑B2 Receptor Tyrosine Kinase 2 (ERBB2) has been identified in several types of cancer, but less is known on its function and mechanism of action in cervical cancer cells. The present study employed a multipronged approach to investigate the role of ERBB2 in cervical cancer. ERBB2 and microRNA (miR)‑3184‑5p expression was assessed in patient‑derived cervical cancer biopsy tissues, revealing that higher levels of ERBB2 and lower levels of miR‑3184‑5p were associated with clinicopathological indicators of cervical cancer progression. Furthermore, ERBB2 stimulated proliferation, migration and sphere‑formation of cervical cancer cells in vitro. This effect was mediated by enhanced phosphatidylinositol‑4,5‑bisphosphate 3‑kinase catalytic subunit α activity. Additionally, it was revealed that miR‑3184‑5p directly suppressed ERBB2 in cervical cancer cells. The p53 activator Mithramycin A stimulated p53 and miR‑3184‑5p expression, thereby lowering the levels of ERBB2 and attenuating proliferation, migration and sphere‑formation of cervical cancer cells. In conclusion, the findings of the present study suggested ERBB2 as an oncogenic protein that may promote invasiveness in cervical cancer cells. Treatment of cervical cancer cells with the p53 activator Mithramycin A restored the levels of the endogenous ERBB2 inhibitor miR‑3184‑5p and may represent a novel treatment strategy for cervical cancer.
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January-2021
Volume 45 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Liu H, Li Y, Zhang J, Wu N, Liu F, Wang L, Zhang Y, Liu J, Zhang X, Guo S, Guo S, et al: Erb‑B2 Receptor Tyrosine Kinase 2 is negatively regulated by the p53‑responsive microRNA‑3184‑5p in cervical cancer cells. Oncol Rep 45: 95-106, 2021
APA
Liu, H., Li, Y., Zhang, J., Wu, N., Liu, F., Wang, L. ... Wang, H. (2021). Erb‑B2 Receptor Tyrosine Kinase 2 is negatively regulated by the p53‑responsive microRNA‑3184‑5p in cervical cancer cells. Oncology Reports, 45, 95-106. https://doi.org/10.3892/or.2020.7862
MLA
Liu, H., Li, Y., Zhang, J., Wu, N., Liu, F., Wang, L., Zhang, Y., Liu, J., Zhang, X., Guo, S., Wang, H."Erb‑B2 Receptor Tyrosine Kinase 2 is negatively regulated by the p53‑responsive microRNA‑3184‑5p in cervical cancer cells". Oncology Reports 45.1 (2021): 95-106.
Chicago
Liu, H., Li, Y., Zhang, J., Wu, N., Liu, F., Wang, L., Zhang, Y., Liu, J., Zhang, X., Guo, S., Wang, H."Erb‑B2 Receptor Tyrosine Kinase 2 is negatively regulated by the p53‑responsive microRNA‑3184‑5p in cervical cancer cells". Oncology Reports 45, no. 1 (2021): 95-106. https://doi.org/10.3892/or.2020.7862