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Long non‑coding RNA ST8SIA6‑AS1 promotes the migration and invasion of hypoxia‑treated hepatocellular carcinoma cells through the miR‑338/MEPCE axis

  • Authors:
    • Bin Zhang
    • Zhiyi Liu
    • Jin Liu
    • Kuan Cao
    • Wengang Shan
    • Quan Wen
    • Renhao Wang
  • View Affiliations / Copyright

    Affiliations: Institute of Digestive Diseases, Xuzhou Medical University; Department of General Surgery, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221000, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 73-82
    |
    Published online on: November 20, 2020
       https://doi.org/10.3892/or.2020.7864
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Abstract

Hepatocellular carcinoma (HCC) is one of the most prevalent types of cancer worldwide. Long non‑coding RNAs (lncRNAs) have been reported to frequently participate in the carcinogenesis and development of various types of cancer, including HCC. However, the molecular mechanisms of lncRNA ST8SIA6‑AS1 in HCC remain poorly understood. The present study performed bioinformatics analysis, in addition to using reverse transcription‑quantitative PCR (RT‑qPCR), nuclear‑cytoplasmic fractionation, RNA immunoprecipitation, and Transwell, wound healing, and dual‑luciferase reporter assays, to determine the biological role and regulatory mechanisms of ST8SIA6‑AS1 in HCC. The results revealed that the expression levels of ST8SIA6‑AS1 were upregulated in HCC tissues and cell lines, which were associated with a poor prognosis. Moreover, the genetic knockdown of ST8SIA6‑AS1 inhibited the hypoxia‑induced HCC cell migration and invasion. Additionally, microRNA (miR)‑338, which exhibited downregulated expression levels in HCC tissues and cell lines, was discovered to bind with ST8SIA6‑AS1. The inhibition of miR‑338 partially reversed the inhibitory effects of ST8SIA6‑AS1‑knockdown on the migration and invasion of HCC cells under hypoxia. Subsequently, methylphosphate capping enzyme (MEPCE) was identified to be targeted and negatively regulated by miR‑338. Notably, the overexpression of MEPCE recovered the inhibitory influence over the migratory and invasive abilities of hypoxia‑treated HCC cells promoted by ST8SIA6‑AS1 inhibition. In conclusion, the findings of the present study suggest that lncRNA ST8SIA6‑AS1 may promote the migration and invasion of hypoxia‑induced HCC cells via the miR‑338/MEPCE axis, indicating a potential diagnostic or therapeutic marker for HCC treatment.
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1 

Banaudha KK and Verma M: Epigenetic biomarkers in liver cancer. Methods Mol Biol. 1238:65–76. 2015. View Article : Google Scholar

2 

Raza A and Sood GK: Hepatocellular carcinoma review: Current treatment, and evidence-based medicine. World J Gastroenterol. 20:4115–4127. 2014. View Article : Google Scholar

3 

Reig M, Mariño Z, Perelló C, Iñarrairaegui M, Ribeiro A, Lens S, Díaz A, Vilana R, Darnell A, Varela M, et al: Unexpected high rate of early tumor recurrence in patients with HCV-related HCC undergoing interferon-free therapy. J Hepatol. 65:719–726. 2016. View Article : Google Scholar

4 

Jarroux J, Morillon A and Pinskaya M: History, discovery, and classification of lncRNAs. Adv Exp Med Biol. 1008:1–46. 2017. View Article : Google Scholar

5 

Kim S, Jeong H, Kim EY, Kim JF, Lee SY and Yoon SH: Genomic and transcriptomic landscape of Escherichia coli BL21(DE3). Nucleic Acids Res. 45:5285–5293. 2017. View Article : Google Scholar

6 

Xu J, Bai J, Zhang X, Lv Y, Gong Y, Liu L, Zhao H, Yu F, Ping Y, Zhang G, et al: A comprehensive overview of lncRNA annotation resources. Brief Bioinform. 18:236–249. 2017.

7 

Tian JB, Cao L and Dong GL: Long noncoding RNA DDX11-AS1 induced by YY1 accelerates colorectal cancer progression through targeting miR-873/CLDN7 axis. Eur Rev Med Pharmacol Sci. 23:5714–5729. 2019.

8 

Xie Q, Lin S, Zheng M, Cai Q and Tu Y: Long noncoding RNA NEAT1 promotes the growth of cervical cancer cells via sponging miR-9-5p. Biochem Cell Biol. 97:100–108. 2019. View Article : Google Scholar

9 

Geisler S and Coller J: RNA in unexpected places: Long non-coding RNA functions in diverse cellular contexts. Nat Rev Mol Cell Biol. 14:699–712. 2013. View Article : Google Scholar

10 

Alvarez-Dominguez JR and Lodish HF: Emerging mechanisms of long noncoding RNA function during normal and malignant hematopoiesis. Blood. 130:1965–1975. 2017. View Article : Google Scholar

11 

Zhu J, Fu H, Wu Y and Zheng X: Function of lncRNAs and approaches to lncRNA-protein interactions. Sci China Life Sci. 56:876–885. 2013. View Article : Google Scholar

12 

Qi X, Zhang DH, Wu N, Xiao JH, Wang X and Ma W: ceRNA in cancer: Possible functions and clinical implications. J Med Genet. 52:710–718. 2015. View Article : Google Scholar

13 

Chiu HS, Rodríguez Martínez M, Bansal M, Subramanian A, Golub TR, Yang X, Sumazin P and Califano A: High-throughput validation of ceRNA regulatory networks. BMC Genomics. 18:4182017. View Article : Google Scholar

14 

Sun Q, Li J, Li F, Li H, Bei S, Zhang X and Feng L: lncRNA LOXL1-AS1 facilitates the tumorigenesis and stemness of gastric carcinoma via regulation of miR-708-5p/USF1 pathway. Cell Prolif. 52:e126872019. View Article : Google Scholar

15 

Yao N, Fu Y, Chen L, Liu Z, He J, Zhu Y, Xia T and Wang S: Long non-coding RNA NONHSAT101069 promotes epirubicin resistance, migration, and invasion of breast cancer cells through NONHSAT101069/miR-129-5p/Twist1 axis. Oncogene. 38:7216–7233. 2019. View Article : Google Scholar

16 

Wojczakowski W, Kobylarek D, Lindner J, Limphaibool N and Kaczmarek M: MicroRNAs-novel biomarkers for malignant pleural effusions. Contemp Oncol (Pozn). 23:133–140. 2019.

17 

Chhatriya B, Mukherjee M, Ray S, Sarkar P, Chatterjee S, Nath D, Das K and Goswami S: Comparison of tumour and serum specific microRNA changes dissecting their role in pancreatic ductal adenocarcinoma: A meta-analysis. BMC Cancer. 19:11752019. View Article : Google Scholar

18 

Liu X and Cui M: miRNA-98-5p inhibits the progression of osteosarcoma by regulating cell cycle via targeting CDC25A expression. Eur Rev Med Pharmacol Sci. 23:9793–9802. 2019.

19 

Yang T, Tian S, Wang L, Wang Y and Zhao J: MicroRNA-367-3p overexpression represses the proliferation and invasion of cervical cancer cells through downregulation of SPAG5-mediated Wnt/β-catenin signaling. Clin Exp Pharmacol Physiol. 47:687–695. 2019. View Article : Google Scholar

20 

Chen DH, Wu QW, Li XD, Wang SJ and Zhang ZM: SYPL1 overexpression predicts poor prognosis of hepatocellular carcinoma and associates with epithelial-mesenchymal transition. Oncol Rep. 38:1533–1542. 2017. View Article : Google Scholar

21 

Zhao ZB, Chen F and Bai XF: Long noncoding RNA MALAT1 regulates hepatocellular carcinoma growth under hypoxia via sponging microRNA-200a. Yonsei Med J. 60:727–734. 2019. View Article : Google Scholar

22 

Liang R, Lin Y, Ye JZ, Yan XX, Liu ZH, Li YQ, Luo XL and Ye HH: High expression of RBM8A predicts poor patient prognosis and promotes tumor progression in hepatocellular carcinoma. Oncol Rep. 37:2167–2176. 2017. View Article : Google Scholar

23 

Xiao J, Lv Y, Jin F, Liu Y, Ma Y, Xiong Y, Liu L, Zhang S, Sun Y, Tipoe GL, et al: lncRNA HANR promotes tumorigenesis and increase of chemoresistance in hepatocellular carcinoma. Cell Physiol Biochem. 43:1926–1938. 2017. View Article : Google Scholar

24 

Huang X, Gao Y, Qin J and Lu S: lncRNA MIAT promotes proliferation and invasion of HCC cells via sponging miR-214. Am J Physiol Gastrointest Liver Physiol. 314:G559–G565. 2018. View Article : Google Scholar

25 

Xu F, Zha G, Wu Y, Cai W and Ao J: Overexpressing lncRNA SNHG16 inhibited HCC proliferation and chemoresistance by functionally sponging hsa-miR-93. Onco Targets Ther. 11:8855–8863. 2018. View Article : Google Scholar

26 

Rui X, Xu Y, Jiang X, Ye W, Huang Y and Jiang J: Long non-coding RNA C5orf66-AS1 promotes cell proliferation in cervical cancer by targeting miR-637/RING1 axis. Cell Death Dis. 9:11752018. View Article : Google Scholar

27 

Wu F, Gao H, Liu K, Gao B, Ren H, Li Z and Liu F: The lncRNA ZEB2-AS1 is upregulated in gastric cancer and affects cell proliferation and invasion via miR-143-5p/HIF-1α axis. Onco Targets Ther. 12:657–667. 2019. View Article : Google Scholar

28 

Tatum JL, Kelloff GJ, Gillies RJ, Arbeit JM, Brown JM, Chao KSC, Chapman JD, Eckelman WC, Fyles AW, Giaccia AJ, et al: Hypoxia: Importance in tumor biology, noninvasive measurement by imaging, and value of its measurement in the management of cancer therapy. Int J Radiat Biol. 82:699–757. 2006. View Article : Google Scholar

29 

Zhang X, Hamblin MH and Yin KJ: The long noncoding RNA Malat1: Its physiological and pathophysiological functions. RNA Biol. 14:1705–1714. 2017. View Article : Google Scholar

30 

Liu D, Li Y, Luo G, Xiao X, Tao D, Wu X, Wang M, Huang C, Wang L, Zeng F and Jiang G: lncRNA SPRY4-IT1 sponges miR-101-3p to promote proliferation and metastasis of bladder cancer cells through up-regulating EZH2. Cancer Lett. 388:281–291. 2017. View Article : Google Scholar

31 

Gan L, Lv L and Liao S: Long noncoding RNA H19 regulates cell growth and metastasis via the miR223p/Snail1 axis in gastric cancer. Int J Oncol. 54:2157–2168. 2019.

32 

Shelton SB, Shah NM, Abell NS, Devanathan SK, Mercado M and Xhemalçe B: Crosstalk between the RNA methylation and histone-binding activities of MePCE regulates P-TEFb activation on chromatin. Cell Rep. 22:1374–1383. 2018. View Article : Google Scholar

33 

Liu R, Wang X, Chen GY, Dalerba P, Gurney A, Hoey T, Sherlock G, Lewicki J, Shedden K and Clarke MF: The prognostic role of a gene signature from tumorigenic breast-cancer cells. N Engl J Med. 356:217–226. 2007. View Article : Google Scholar

34 

Yao L, Chi Y, Hu X, Li S, Qiao F, Wu J and Shao ZM: Elevated expression of RNA methyltransferase BCDIN3D predicts poor prognosis in breast cancer. Oncotarget. 7:53895–53902. 2016. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Zhang B, Liu Z, Liu J, Cao K, Shan W, Wen Q and Wang R: Long non‑coding RNA ST8SIA6‑AS1 promotes the migration and invasion of hypoxia‑treated hepatocellular carcinoma cells through the miR‑338/MEPCE axis. Oncol Rep 45: 73-82, 2021.
APA
Zhang, B., Liu, Z., Liu, J., Cao, K., Shan, W., Wen, Q., & Wang, R. (2021). Long non‑coding RNA ST8SIA6‑AS1 promotes the migration and invasion of hypoxia‑treated hepatocellular carcinoma cells through the miR‑338/MEPCE axis. Oncology Reports, 45, 73-82. https://doi.org/10.3892/or.2020.7864
MLA
Zhang, B., Liu, Z., Liu, J., Cao, K., Shan, W., Wen, Q., Wang, R."Long non‑coding RNA ST8SIA6‑AS1 promotes the migration and invasion of hypoxia‑treated hepatocellular carcinoma cells through the miR‑338/MEPCE axis". Oncology Reports 45.1 (2021): 73-82.
Chicago
Zhang, B., Liu, Z., Liu, J., Cao, K., Shan, W., Wen, Q., Wang, R."Long non‑coding RNA ST8SIA6‑AS1 promotes the migration and invasion of hypoxia‑treated hepatocellular carcinoma cells through the miR‑338/MEPCE axis". Oncology Reports 45, no. 1 (2021): 73-82. https://doi.org/10.3892/or.2020.7864
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang B, Liu Z, Liu J, Cao K, Shan W, Wen Q and Wang R: Long non‑coding RNA ST8SIA6‑AS1 promotes the migration and invasion of hypoxia‑treated hepatocellular carcinoma cells through the miR‑338/MEPCE axis. Oncol Rep 45: 73-82, 2021.
APA
Zhang, B., Liu, Z., Liu, J., Cao, K., Shan, W., Wen, Q., & Wang, R. (2021). Long non‑coding RNA ST8SIA6‑AS1 promotes the migration and invasion of hypoxia‑treated hepatocellular carcinoma cells through the miR‑338/MEPCE axis. Oncology Reports, 45, 73-82. https://doi.org/10.3892/or.2020.7864
MLA
Zhang, B., Liu, Z., Liu, J., Cao, K., Shan, W., Wen, Q., Wang, R."Long non‑coding RNA ST8SIA6‑AS1 promotes the migration and invasion of hypoxia‑treated hepatocellular carcinoma cells through the miR‑338/MEPCE axis". Oncology Reports 45.1 (2021): 73-82.
Chicago
Zhang, B., Liu, Z., Liu, J., Cao, K., Shan, W., Wen, Q., Wang, R."Long non‑coding RNA ST8SIA6‑AS1 promotes the migration and invasion of hypoxia‑treated hepatocellular carcinoma cells through the miR‑338/MEPCE axis". Oncology Reports 45, no. 1 (2021): 73-82. https://doi.org/10.3892/or.2020.7864
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