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Article Open Access

TELO2 induced progression of colorectal cancer by binding with RICTOR through mTORC2

  • Authors:
    • Zheng Guo
    • Xiufang Zhang
    • Huabin Zhu
    • Nanying Zhong
    • Xiaoning Luo
    • Yu Zhang
    • Fuping Tu
    • Jinghua Zhong
    • Xiangcai Wang
    • Jue He
    • Li Huang
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, First Affiliated Hospital of Gannan Medical University, Gannan Medical University, Ganzhou, Jiangxi 341000, P.R. China, First School of Clinical Medicine, Gannan Medical University, Ganzhou, Jiangxi 341000, P.R. China, Department of Pathology, First Affiliated Hospital of Gannan Medical University, Gannan Medical University, Ganzhou, Jiangxi 341000, P.R. China
    Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 523-534
    |
    Published online on: December 9, 2020
       https://doi.org/10.3892/or.2020.7890
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Abstract

Colorectal cancer (CRC) is a common cancer worldwide, and its treatment strategies are limited. The underlying mechanism of CRC progression remains to be determined. Telomere maintenance 2 (TELO2) is a mTOR‑interacting protein. Both the role and molecular mechanism of TELO2 in cancer progression remain unknown. In this study, the gene expression database of normal and tumor tissue, in addition to western blot analysis, and immunohistochemistry (IHC) were used to determine the expression and location of TELO2 in CRC and normal tissues. Clinical features of a tissue array were collected and analyzed. WST‑1, soft agar, flow cytometry, wound healing, and invasion assays were employed to verify the role of TELO2 in the growth, cell cycle, migration, and invasion of CRC cells. The correlation between TELO2 and RICTOR (rapamycin‑insensitive companion of mTOR) was analyzed by bioinformatics, IHC, and immunoprecipitation. Normal and serum‑deprived cells were collected to detect the protein level of TELO2 and its downstream effectors. The results revealed that TELO2 was significantly upregulated in CRC, and TELO2 inhibition significantly restrained the growth, cell cycle, and metastasis of CRC cells. TELO2 overexpression correlated with age, lymph node metastasis, and TNM stage of CRC patients. In addition, TELO2 was positively correlated with RICTOR in CRC and induced tumor progression mainly via RICTOR with serum in culture. RICTOR induced the degradation of TELO2 upon serum deprivation in an mTOR‑independent manner. These findings indicate that TELO2 promotes tumor progression via RICTOR in a serum‑dependent manner, which may be a potential therapeutic target for CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Guo Z, Zhang X, Zhu H, Zhong N, Luo X, Zhang Y, Tu F, Zhong J, Wang X, He J, He J, et al: TELO2 induced progression of colorectal cancer by binding with RICTOR through mTORC2. Oncol Rep 45: 523-534, 2021.
APA
Guo, Z., Zhang, X., Zhu, H., Zhong, N., Luo, X., Zhang, Y. ... Huang, L. (2021). TELO2 induced progression of colorectal cancer by binding with RICTOR through mTORC2. Oncology Reports, 45, 523-534. https://doi.org/10.3892/or.2020.7890
MLA
Guo, Z., Zhang, X., Zhu, H., Zhong, N., Luo, X., Zhang, Y., Tu, F., Zhong, J., Wang, X., He, J., Huang, L."TELO2 induced progression of colorectal cancer by binding with RICTOR through mTORC2". Oncology Reports 45.2 (2021): 523-534.
Chicago
Guo, Z., Zhang, X., Zhu, H., Zhong, N., Luo, X., Zhang, Y., Tu, F., Zhong, J., Wang, X., He, J., Huang, L."TELO2 induced progression of colorectal cancer by binding with RICTOR through mTORC2". Oncology Reports 45, no. 2 (2021): 523-534. https://doi.org/10.3892/or.2020.7890
Copy and paste a formatted citation
x
Spandidos Publications style
Guo Z, Zhang X, Zhu H, Zhong N, Luo X, Zhang Y, Tu F, Zhong J, Wang X, He J, He J, et al: TELO2 induced progression of colorectal cancer by binding with RICTOR through mTORC2. Oncol Rep 45: 523-534, 2021.
APA
Guo, Z., Zhang, X., Zhu, H., Zhong, N., Luo, X., Zhang, Y. ... Huang, L. (2021). TELO2 induced progression of colorectal cancer by binding with RICTOR through mTORC2. Oncology Reports, 45, 523-534. https://doi.org/10.3892/or.2020.7890
MLA
Guo, Z., Zhang, X., Zhu, H., Zhong, N., Luo, X., Zhang, Y., Tu, F., Zhong, J., Wang, X., He, J., Huang, L."TELO2 induced progression of colorectal cancer by binding with RICTOR through mTORC2". Oncology Reports 45.2 (2021): 523-534.
Chicago
Guo, Z., Zhang, X., Zhu, H., Zhong, N., Luo, X., Zhang, Y., Tu, F., Zhong, J., Wang, X., He, J., Huang, L."TELO2 induced progression of colorectal cancer by binding with RICTOR through mTORC2". Oncology Reports 45, no. 2 (2021): 523-534. https://doi.org/10.3892/or.2020.7890
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