Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1021-335X Online ISSN: 1791-2431
Journal Cover
March-2021 Volume 45 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
March-2021 Volume 45 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML

  • Supplementary Files
    • Supplementary_Data.pdf
Article Open Access

Waterpipe smoke condensate influences epithelial to mesenchymal transition and interferes with the cytotoxic immune response in non-small cell lung cancer cell lines

  • Authors:
    • Rania Faouzi Zaarour
    • Prathibha Prasad
    • Goutham Hassan Venkatesh
    • Raefa Abou Khouzam
    • Francis Amirtharaj
    • Nagwa Zeinelabdin
    • Ayesha Rifath
    • Stephane Terry
    • Husam Nawafleh
    • Yehya El Sayed
    • Salem Chouaib
  • View Affiliations / Copyright

    Affiliations: Thumbay Research Institute for Precision Medicine, Gulf Medical University, Ajman 4184, UAE, INSERM UMR 1186, Integrative Tumour Immunology and Immunotherapy, Gustave Roussy, Faculty of Medicine - University of Paris-Sud, University of Paris-Saclay, F-94805 Villejuif, France, Department of Biology, Chemistry and Environmental Sciences (BCE), American University of Sharjah, Sharjah 26666, UAE
    Copyright: © Zaarour et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 879-890
    |
    Published online on: January 18, 2021
       https://doi.org/10.3892/or.2021.7938
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Waterpipe tobacco smoking (WPS) continues to spread globally and presents serious health hazards. The aim of the present study was to investigate the effects of treatment with WPS condensate (WPSC) on lung cell proliferation and plasticity as well as tumor cell recognition and killing by natural killer (NK) cells using cytotoxicity assays. The results indicated that exposure of normal and cancer lung cell lines to WPSC resulted in a decrease in their in vitro growth in a dose-dependent manner and it induced tumor senescence. In addition, WPSC selectively caused DNA damage as revealed by an increase in γH2AX and 53BP1 in tumor lung cells. To gain further insight into the molecular mechanisms altered by WPSC, we conducted a global comprehensive transcriptome analysis of WPSC-treated tumor cells. Data analysis identified an expression profile of genes that best distinguished treated and non-treated cells involving several pathways. Of these pathways, we focused on those involved in epithelial to mesenchymal transition (EMT) and stemness. Results showed that WPSC induced an increase in SNAI2 expression associated with EMT, ACTA2 and SERPINE2 were involved in invasion and CD44 was associated with stemness. Furthermore, WPSC exposure increased the expression of inflammatory response genes including CASP1, IL1B, IL6 and CCL2. While immune synapse formation between NK and WPSC-treated lung cancer target cells was not affected, the capacity of NK cells to kill these target cells was reduced. The data reported in the present study are, to the best of our knowledge, the first in vitro demonstration of WPSC effects on lung cellular parameters providing evidence of its potential involvement in tumor physiology and development.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Polverino F, Sam A and Guerra S: COPD: To Be or Not to Be, That is the Question. Am J Med. 132:1271–1278. 2019. View Article : Google Scholar : PubMed/NCBI

2 

Montazeri Z, Nyiraneza C, El-Katerji H and Little J: Waterpipe smoking and cancer: Systematic review and meta-analysis. Tob Control. 26:92–97. 2017. View Article : Google Scholar : PubMed/NCBI

3 

Talhout R, Schulz T, Florek E, van Benthem J, Wester P and Opperhuizen A: Hazardous compounds in tobacco smoke. Int J Environ Res Public Health. 8:613–628. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Shihadeh A, Schubert J, Klaiany J, El Sabban M, Luch A and Saliba NA: Toxicant content, physical properties and biological activity of waterpipe tobacco smoke and its tobacco-free alternatives. Tob Control. 24 (Suppl 1):i22–i30. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Maziak W, Jawad M, Jawad S, Ward KD, Eissenberg T and Asfar T: Interventions for waterpipe smoking cessation. Cochrane Database Syst Rev (7). CD0055492015.

6 

Etemadi A, Poustchi H, Chang CM, Blount BC, Calafat AM, Wang L, De Jesus VR, Pourshams A, Shakeri R, Shiels MS, et al: Urinary biomarkers of carcinogenic exposure among cigarette, waterpipe, and smokeless tobacco users and never users of tobacco in the Golestan Cohort Study. Cancer Epidemiol Biomarkers Prev. 28:337–347. 2019. View Article : Google Scholar : PubMed/NCBI

7 

Boskabady MH, Farhang L, Mahmodinia M, Boskabady M and Heydari GR: Comparison of pulmonary function and respiratory symptoms in water pipe and cigarette smokers. Respirology. 17:950–956. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Awan KH, Siddiqi K, Patil Sh and Hussain QA: Assessing the affect of waterpipe smoking on cancer outcome - a systematic review of current evidence. Asian Pac J Cancer Prev. 18:495–502. 2017.PubMed/NCBI

9 

Alsaad AM, Al-Arifi MN, Maayah ZH, Attafi IM, Alanazi FE, Belali OM, Alhoshani A, Asiri YA and Korashy HM: Genotoxic impact of long-term cigarette and waterpipe smoking on DNA damage and oxidative stress in healthy subjects. Toxicol Mech Methods. 29:119–127. 2019. View Article : Google Scholar : PubMed/NCBI

10 

Rammah M, Dandachi F, Salman R, Shihadeh A and El-Sabban M: In vitro cytotoxicity and mutagenicity of mainstream waterpipe smoke and its functional consequences on alveolar type II derived cells. Toxicol Lett. 211:220–231. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Bodas M, Van Westphal C, Carpenter-Thompson R, K Mohanty D and Vij N: Nicotine exposure induces bronchial epithelial cell apoptosis and senescence via ROS mediated autophagy-impairment. Free Radic Biol Med. 97:441–453. 2016. View Article : Google Scholar : PubMed/NCBI

12 

Rammah M, Dandachi F, Salman R, Shihadeh A and El-Sabban M: In vitro effects of waterpipe smoke condensate on endothelial cell function: A potential risk factor for vascular disease. Toxicol Lett. 219:133–142. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Gerlinger M, Rowan AJ, Horswell S, Math M, Larkin J, Endesfelder D, Gronroos E, Martinez P, Matthews N, Stewart A, et al: Intratumor heterogeneity and branched evolution revealed by multiregion sequencing. N Engl J Med. 366:883–892. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Elsayed Y, Dalibalta S and Abu-Farha N: Chemical analysis and potential health risks of hookah charcoal. Sci Total Environ. 569-570:262–268. 2016. View Article : Google Scholar : PubMed/NCBI

15 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Olive PL and Banáth JP: The comet assay: A method to measure DNA damage in individual cells. Nat Protoc. 1:23–29. 2006. View Article : Google Scholar : PubMed/NCBI

17 

Gyori BM, Venkatachalam G, Thiagarajan PS, Hsu D and Clement MV: OpenComet: An automated tool for comet assay image analysis. Redox Biol. 2:457–465. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Terry S, Abdou A, Engelsen AST, Buart S, Dessen P, Corgnac S, Collares D, Meurice G, Gausdal G, Baud V, et al: AXL Targeting overcomes human lung cancer cell resistance to NK- and CTL-mediated cytotoxicity. Cancer Immunol Res. 7:1789–1802. 2019. View Article : Google Scholar : PubMed/NCBI

19 

Babicki S, Arndt D, Marcu A, Liang Y, Grant JR, Maciejewski A and Wishart DS: Heatmapper: Web-enabled heat mapping for all. Nucleic Acids Res. 44((W1)): W147–53. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Oliveros JC: An interactive tool for comparing lists with Venns diagrams. Publicly available at. http://bioinfogp.cnb.csic.es/tools/venny/index.html

21 

Subramanian A, Tamayo P, Mootha VK, Mukherjee S, Ebert BL, Gillette MA, Paulovich A, Pomeroy SL, Golub TR, Lander ES, et al: Gene set enrichment analysis: A knowledge-based approach for interpreting genome-wide expression profiles. Proc Natl Acad Sci USA. 102:15545–15550. 2005. View Article : Google Scholar : PubMed/NCBI

22 

Liberzon A, Birger C, Thorvaldsdóttir H, Ghandi M, Mesirov JP and Tamayo P: The Molecular Signatures Database (MSigDB) hallmark gene set collection. Cell Syst. 1:417–425. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Durling LJ, Busk L and Hellman BE: Evaluation of the DNA damaging effect of the heat-induced food toxicant 5-hydroxymethylfurfural (HMF) in various cell lines with different activities of sulfotransferases. Food Chem Toxicol. 47:880–884. 2009. View Article : Google Scholar : PubMed/NCBI

24 

Kurashige T, Shimamura M and Nagayama Y: Differences in quantification of DNA double-strand breaks assessed by 53BP1/γH2AX focus formation assays and the comet assay in mammalian cells treated with irradiation and N-acetyl-L-cysteine. J Radiat Res (Tokyo). 57:312–317. 2016. View Article : Google Scholar

25 

Kumari B, Aslam SK, Zaheer S, Adil SO and Shafique K: Systemic inflammatory markers among waterpipe smokers, cigarette smokers, and nonsmokers. J Addict Med. 13:55–60. 2019. View Article : Google Scholar : PubMed/NCBI

26 

Apte RN, Dotan S, Elkabets M, White MR, Reich E, Carmi Y, Song X, Dvozkin T, Krelin Y and Voronov E: The involvement of IL-1 in tumorigenesis, tumor invasiveness, metastasis and tumor-host interactions. Cancer Metastasis Rev. 25:387–408. 2006. View Article : Google Scholar : PubMed/NCBI

27 

Li M, Knight DA, A Snyder L, Smyth MJ and Stewart TJ: A role for CCL2 in both tumor progression and immunosurveillance. OncoImmunology. 2:e254742013. View Article : Google Scholar : PubMed/NCBI

28 

Browning L, Patel MR, Horvath EB, Tawara K and Jorcyk CL: IL-6 and ovarian cancer: Inflammatory cytokines in promotion of metastasis. Cancer Manag Res. 10:6685–6693. 2018. View Article : Google Scholar : PubMed/NCBI

29 

Siegmund B, Lehr HA, Fantuzzi G and Dinarello CA: IL-1 beta -converting enzyme (caspase-1) in intestinal inflammation. Proc Natl Acad Sci USA. 98:13249–13254. 2001. View Article : Google Scholar : PubMed/NCBI

30 

Mani SA, Guo W, Liao MJ, Eaton EN, Ayyanan A, Zhou AY, Brooks M, Reinhard F, Zhang CC, Shipitsin M, et al: The epithelial-mesenchymal transition generates cells with properties of stem cells. Cell. 133:704–715. 2008. View Article : Google Scholar : PubMed/NCBI

31 

Bhattacharya R, Mitra T, Ray Chaudhuri S and Roy SS: Mesenchymal splice isoform of CD44 (CD44s) promotes EMT/invasion and imparts stem-like properties to ovarian cancer cells. J Cell Biochem. 119:3373–3383. 2018. View Article : Google Scholar : PubMed/NCBI

32 

Jayachandran A, Königshoff M, Yu H, Rupniewska E, Hecker M, Klepetko W, Seeger W and Eickelberg O: SNAI transcription factors mediate epithelial-mesenchymal transition in lung fibrosis. Thorax. 64:1053–1061. 2009. View Article : Google Scholar : PubMed/NCBI

33 

Hindriksen S and Bijlsma MF: Cancer stem cells, EMT, and developmental pathway activation in pancreatic tumors. Cancers (Basel). 4:989–1035. 2012. View Article : Google Scholar : PubMed/NCBI

34 

Tomaskovic-Crook E, Thompson EW and Thiery JP: Epithelial to mesenchymal transition and breast cancer. Breast Cancer Res. 11:2132009. View Article : Google Scholar : PubMed/NCBI

35 

Yoshida K, Gowers KHC, Lee-Six H, Chandrasekharan DP, Coorens T, Maughan EF, Beal K, Menzies A, Millar FR, Anderson E, et al: Tobacco smoking and somatic mutations in human bronchial epithelium. Nature. 578:266–272. 2020. View Article : Google Scholar : PubMed/NCBI

36 

Willers H, Gheorghiu L, Liu Q, Efstathiou JA, Wirth LJ, Krause M and von Neubeck C: DNA damage response assessments in human tumor samples provide functional biomarkers of radiosensitivity. Semin Radiat Oncol. 25:237–250. 2015. View Article : Google Scholar : PubMed/NCBI

37 

Holcomb N, Goswami M, Han SG, Clark S, Orren DK, Gairola CG and Mellon I: Exposure of human lung cells to tobacco smoke condensate inhibits the nucleotide excision repair pathway. PLoS One. 11:e01588582016. View Article : Google Scholar : PubMed/NCBI

38 

Hang B, Sarker AH, Havel C, Saha S, Hazra TK, Schick S, Jacob P III, Rehan VK, Chenna A, Sharan D, et al: Thirdhand smoke causes DNA damage in human cells. Mutagenesis. 28:381–391. 2013. View Article : Google Scholar : PubMed/NCBI

39 

Lowe DB and Storkus WJ: Chronic inflammation and immunologic-based constraints in malignant disease. Immunotherapy. 3:1265–1274. 2011. View Article : Google Scholar : PubMed/NCBI

40 

Jeong YJ, Oh HK, Park SH and Bong JG: Association between inflammation and cancer stem cell phenotype in breast cancer. Oncol Lett. 15:2380–2386. 2018.PubMed/NCBI

41 

Masola V, Carraro A, Granata S, Signorini L, Bellin G, Violi P, Lupo A, Tedeschi U, Onisto M, Gambaro G, et al: In vitro effects of interleukin (IL)-1 beta inhibition on the epithelial-to-mesenchymal transition (EMT) of renal tubular and hepatic stellate cells. J Transl Med. 17:122019. View Article : Google Scholar : PubMed/NCBI

42 

Ling Z, Yang X, Chen X, Xia J, Cheng B and Tao X: CCL2 promotes cell migration by inducing epithelial-mesenchymal transition in oral squamous cell carcinoma. J Oral Pathol Med. 48:477–482. 2019. View Article : Google Scholar : PubMed/NCBI

43 

Li J, Zhou Y, Liu Y, Dai B, Zhang YH, Zhang PF and Shi XL: Sorafenib inhibits caspase-1 expression through suppressing TLR4/stat3/SUMO1 pathway in hepatocellular carcinoma. Cancer Biol Ther. 19:1057–1064. 2018. View Article : Google Scholar : PubMed/NCBI

44 

Coppé JP, Patil CK, Rodier F, Sun Y, Muñoz DP, Goldstein J, Nelson PS, Desprez PY and Campisi J: Senescence-associated secretory phenotypes reveal cell-nonautonomous functions of oncogenic RAS and the p53 tumor suppressor. PLoS Biol. 6:2853–2868. 2008. View Article : Google Scholar : PubMed/NCBI

45 

Khan NA, Lawyer G, McDonough S, Wang Q, Kassem NO, Kas-Petrus F, Ye D, Singh KP, Kassem NOF and Rahman I: Systemic biomarkers of inflammation, oxidative stress and tissue injury and repair among waterpipe, cigarette and dual tobacco smokers. Tob Control. 2019.PubMed/NCBI

46 

Qasim H, Alarabi AB, Alzoubi KH, Karim ZA, Alshbool FZ and Khasawneh FT: The effects of hookah/waterpipe smoking on general health and the cardiovascular system. Environ Health Prev Med. 24:582019. View Article : Google Scholar : PubMed/NCBI

47 

Sadek KW, Haik MY, Ashour AA, Baloch T, Aboulkassim T, Yasmeen A, Vranic S, Zeidan A and Al Moustafa AE: Water-pipe smoking promotes epithelial-mesenchymal transition and invasion of human breast cancer cells via ERK1/ERK2 pathways. Cancer Cell Int. 18:1802018. View Article : Google Scholar : PubMed/NCBI

48 

Patil S, Subbannayya T, Mohan SV, Babu N, Advani J, Sathe G, Rajagopalan P, Patel K, Bhandi S, Solanki H, et al: Proteomic changes in oral keratinocytes chronically exposed to Shisha (Water Pipe). OMICS. 23:86–97. 2019. View Article : Google Scholar : PubMed/NCBI

49 

Reyes-Caballero H, Park B, Loube J, Sanchez I, Vinayachandran V, Choi Y, Woo J, Edwards J, Brinkman MC, Sussan T, et al: Immune modulation by chronic exposure to waterpipe smoke and immediate-early gene regulation in murine lungs. Tob Control. 2019.PubMed/NCBI

50 

Tollerud DJ, Clark JW, Brown LM, Neuland CY, Mann DL, Pankiw-Trost LK, Blattner WA and Hoover RN: Association of cigarette smoking with decreased numbers of circulating natural killer cells. Am Rev Respir Dis. 139:194–198. 1989. View Article : Google Scholar : PubMed/NCBI

51 

Phillips B, Marshall ME, Brown S and Thompson JS: Effect of smoking on human natural killer cell activity. Cancer. 56:2789–2792. 1985. View Article : Google Scholar : PubMed/NCBI

52 

Newman LS, Kreiss K and Campbell PA: Natural killer cell tumoricidal activity in cigarette smokers and in silicotics. Clin Immunol Immunopathol. 60:399–411. 1991. View Article : Google Scholar : PubMed/NCBI

53 

Motz GT, Eppert BL, Wortham BW, Amos-Kroohs RM, Flury JL, Wesselkamper SC and Borchers MT: Chronic cigarette smoke exposure primes NK cell activation in a mouse model of chronic obstructive pulmonary disease. J Immunol. 184:4460–4469. 2010. View Article : Google Scholar : PubMed/NCBI

54 

Vu T, Jin L and Datta PK: Effect of cigarette smoking on epithelial to mesenchymal transition (EMT) in lung cancer. J Clin Med. 5:E442016. View Article : Google Scholar : PubMed/NCBI

55 

Hoshino Y, Mio T, Nagai S, Miki H, Ito I and Izumi T: Cytotoxic effects of cigarette smoke extract on an alveolar type II cell-derived cell line. Am J Physiol Lung Cell Mol Physiol. 281:L509–L516. 2001. View Article : Google Scholar : PubMed/NCBI

56 

Jassem J: Tobacco smoking after diagnosis of cancer: Clinical aspects. Transl Lung Cancer Res. 8 (Suppl 1):S50–S58. 2019. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zaarour RF, Prasad P, Venkatesh GH, Khouzam RA, Amirtharaj F, Zeinelabdin N, Rifath A, Terry S, Nawafleh H, El Sayed Y, El Sayed Y, et al: Waterpipe smoke condensate influences epithelial to mesenchymal transition and interferes with the cytotoxic immune response in non-small cell lung cancer cell lines. Oncol Rep 45: 879-890, 2021.
APA
Zaarour, R.F., Prasad, P., Venkatesh, G.H., Khouzam, R.A., Amirtharaj, F., Zeinelabdin, N. ... Chouaib, S. (2021). Waterpipe smoke condensate influences epithelial to mesenchymal transition and interferes with the cytotoxic immune response in non-small cell lung cancer cell lines. Oncology Reports, 45, 879-890. https://doi.org/10.3892/or.2021.7938
MLA
Zaarour, R. F., Prasad, P., Venkatesh, G. H., Khouzam, R. A., Amirtharaj, F., Zeinelabdin, N., Rifath, A., Terry, S., Nawafleh, H., El Sayed, Y., Chouaib, S."Waterpipe smoke condensate influences epithelial to mesenchymal transition and interferes with the cytotoxic immune response in non-small cell lung cancer cell lines". Oncology Reports 45.3 (2021): 879-890.
Chicago
Zaarour, R. F., Prasad, P., Venkatesh, G. H., Khouzam, R. A., Amirtharaj, F., Zeinelabdin, N., Rifath, A., Terry, S., Nawafleh, H., El Sayed, Y., Chouaib, S."Waterpipe smoke condensate influences epithelial to mesenchymal transition and interferes with the cytotoxic immune response in non-small cell lung cancer cell lines". Oncology Reports 45, no. 3 (2021): 879-890. https://doi.org/10.3892/or.2021.7938
Copy and paste a formatted citation
x
Spandidos Publications style
Zaarour RF, Prasad P, Venkatesh GH, Khouzam RA, Amirtharaj F, Zeinelabdin N, Rifath A, Terry S, Nawafleh H, El Sayed Y, El Sayed Y, et al: Waterpipe smoke condensate influences epithelial to mesenchymal transition and interferes with the cytotoxic immune response in non-small cell lung cancer cell lines. Oncol Rep 45: 879-890, 2021.
APA
Zaarour, R.F., Prasad, P., Venkatesh, G.H., Khouzam, R.A., Amirtharaj, F., Zeinelabdin, N. ... Chouaib, S. (2021). Waterpipe smoke condensate influences epithelial to mesenchymal transition and interferes with the cytotoxic immune response in non-small cell lung cancer cell lines. Oncology Reports, 45, 879-890. https://doi.org/10.3892/or.2021.7938
MLA
Zaarour, R. F., Prasad, P., Venkatesh, G. H., Khouzam, R. A., Amirtharaj, F., Zeinelabdin, N., Rifath, A., Terry, S., Nawafleh, H., El Sayed, Y., Chouaib, S."Waterpipe smoke condensate influences epithelial to mesenchymal transition and interferes with the cytotoxic immune response in non-small cell lung cancer cell lines". Oncology Reports 45.3 (2021): 879-890.
Chicago
Zaarour, R. F., Prasad, P., Venkatesh, G. H., Khouzam, R. A., Amirtharaj, F., Zeinelabdin, N., Rifath, A., Terry, S., Nawafleh, H., El Sayed, Y., Chouaib, S."Waterpipe smoke condensate influences epithelial to mesenchymal transition and interferes with the cytotoxic immune response in non-small cell lung cancer cell lines". Oncology Reports 45, no. 3 (2021): 879-890. https://doi.org/10.3892/or.2021.7938
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team