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Article

Low microRNA150 expression is associated with activated carcinogenic pathways and a poor prognosis in patients with breast cancer

  • Authors:
    • San‑Qi An
    • Ke He
    • Fei Liu
    • Qian‑Shan Ding
    • Yan‑Li Wei
    • Zheng‑Lin Xia
    • Xiao‑Peng Duan
    • Rui Huang
    • Bo‑Wei Li
    • Hai‑He Wang
    • Yu Tian
    • Guo‑An Xiang
    • Wen‑Xing Li
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Guangdong Second Provincial General Hospital, Guangzhou, Guangdong 510317, P.R. China, Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China, Henan Cancer Hospital, Zhengzhou, Henan 450000, P.R. China, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, P.R. China
  • Pages: 1235-1248
    |
    Published online on: January 21, 2021
       https://doi.org/10.3892/or.2021.7945
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Abstract

Breast cancer is the most common type of cancer amongst women worldwide, and numerous microRNAs (miRNAs/miRs) are involved in the initiation and progression of breast cancer. The aim of the present study was to identify hub miRNAs and determine the underlying mechanisms regulated by these miRNAs in breast cancer. Breast invasive carcinoma transcriptome data (including mRNAs and miRNAs), and clinical data were acquired from The Cancer Genome Atlas database. Differential gene expression analysis, co‑expression network analysis, gene set enrichment analysis (GSEA) and prognosis analysis were used to screen the hub miRNAs and explore their functions. Functional experiments were used to determine the underlying mechanisms of the hub miRNAs in breast cancer cells. The results revealed that low miR150 expression predicted a more advanced disease stage, and was associated with a less favorable prognosis. Through the combined use of five miRNA‑target gene prediction tools, 31 potential miR150 target genes were identified. GSEA revealed that low miR150 expression was associated with the upregulation of several cancer‑associated signaling pathways, and the downregulation of several tumor suppressor genes. Furthermore, miR150 independently affected overall survival in patients, and interacted with its target genes to indirectly affect overall and disease‑free survival. Functional experiments demonstrated that miR150 positively regulated B and T lymphocyte attenuator (BTLA), and the downregulation of miR150 and BTLA combined promoted cell migration. In conclusion, the present study revealed that low miR150 expression was associated with less favorable clinical features, upregulation of several carcinogenic signaling pathways, and poor patient survival. Additionally, a miR150‑BTLA axis was suggested to regulate cell viability and migration.
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Copy and paste a formatted citation
Spandidos Publications style
An SQ, He K, Liu F, Ding QS, Wei YL, Xia ZL, Duan XP, Huang R, Li BW, Wang HH, Wang HH, et al: Low microRNA150 expression is associated with activated carcinogenic pathways and a poor prognosis in patients with breast cancer. Oncol Rep 45: 1235-1248, 2021.
APA
An, S., He, K., Liu, F., Ding, Q., Wei, Y., Xia, Z. ... Li, W. (2021). Low microRNA150 expression is associated with activated carcinogenic pathways and a poor prognosis in patients with breast cancer. Oncology Reports, 45, 1235-1248. https://doi.org/10.3892/or.2021.7945
MLA
An, S., He, K., Liu, F., Ding, Q., Wei, Y., Xia, Z., Duan, X., Huang, R., Li, B., Wang, H., Tian, Y., Xiang, G., Li, W."Low microRNA150 expression is associated with activated carcinogenic pathways and a poor prognosis in patients with breast cancer". Oncology Reports 45.3 (2021): 1235-1248.
Chicago
An, S., He, K., Liu, F., Ding, Q., Wei, Y., Xia, Z., Duan, X., Huang, R., Li, B., Wang, H., Tian, Y., Xiang, G., Li, W."Low microRNA150 expression is associated with activated carcinogenic pathways and a poor prognosis in patients with breast cancer". Oncology Reports 45, no. 3 (2021): 1235-1248. https://doi.org/10.3892/or.2021.7945
Copy and paste a formatted citation
x
Spandidos Publications style
An SQ, He K, Liu F, Ding QS, Wei YL, Xia ZL, Duan XP, Huang R, Li BW, Wang HH, Wang HH, et al: Low microRNA150 expression is associated with activated carcinogenic pathways and a poor prognosis in patients with breast cancer. Oncol Rep 45: 1235-1248, 2021.
APA
An, S., He, K., Liu, F., Ding, Q., Wei, Y., Xia, Z. ... Li, W. (2021). Low microRNA150 expression is associated with activated carcinogenic pathways and a poor prognosis in patients with breast cancer. Oncology Reports, 45, 1235-1248. https://doi.org/10.3892/or.2021.7945
MLA
An, S., He, K., Liu, F., Ding, Q., Wei, Y., Xia, Z., Duan, X., Huang, R., Li, B., Wang, H., Tian, Y., Xiang, G., Li, W."Low microRNA150 expression is associated with activated carcinogenic pathways and a poor prognosis in patients with breast cancer". Oncology Reports 45.3 (2021): 1235-1248.
Chicago
An, S., He, K., Liu, F., Ding, Q., Wei, Y., Xia, Z., Duan, X., Huang, R., Li, B., Wang, H., Tian, Y., Xiang, G., Li, W."Low microRNA150 expression is associated with activated carcinogenic pathways and a poor prognosis in patients with breast cancer". Oncology Reports 45, no. 3 (2021): 1235-1248. https://doi.org/10.3892/or.2021.7945
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