Open Access

miR‑146b‑5p inhibits tumorigenesis and metastasis of gallbladder cancer by targeting Toll‑like receptor 4 via the nuclear factor‑κB pathway

  • Authors:
    • Bin Ouyang
    • Ningfeng Pan
    • Haifeng Zhang
    • Chuanming Xing
    • Wu Ji
  • View Affiliations

  • Published online on: February 3, 2021     https://doi.org/10.3892/or.2021.7966
  • Article Number: 15
  • Copyright: © Ouyang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Gallbladder cancer (GBC) is a carcinoma of the biliary tract, which is common in developing countries and is associated with a high fatality rate. The aim of the present study was to investigate the mechanisms underlying the occurrence and development of GBC. A decrease in the expression of miR‑146b‑5p and an increase in the expression of its target gene Toll‑like receptor 4 (TLR4) were first observed in GBC tissues. Further study demonstrated that an increase in TLR4 expression caused by a decrease in miR‑146b‑5p expression led to activation of nuclear factor (NF)‑κB signaling. GBC cells were cultured in vitro, and it was observed that overexpression of miR‑146b‑5p effectively inhibited their viability, proliferation, migration and invasion, and increased their apoptosis. Using a BALB/c nude mouse xenograft model, it was demonstrated that overexpression of miR‑146b‑5p was sufficient to reduce tumor volume and alleviate pathological characteristics. Overall, the results of the present study indicated that the decrease in the expression of miR‑146b‑5p increased TLR4 expression and indirectly activated the NF‑κB signaling pathway, thereby regulating the development of GBC.
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April-2021
Volume 45 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Ouyang B, Pan N, Zhang H, Xing C and Ji W: miR‑146b‑5p inhibits tumorigenesis and metastasis of gallbladder cancer by targeting Toll‑like receptor 4 via the nuclear factor‑κB pathway. Oncol Rep 45: 15, 2021
APA
Ouyang, B., Pan, N., Zhang, H., Xing, C., & Ji, W. (2021). miR‑146b‑5p inhibits tumorigenesis and metastasis of gallbladder cancer by targeting Toll‑like receptor 4 via the nuclear factor‑κB pathway. Oncology Reports, 45, 15. https://doi.org/10.3892/or.2021.7966
MLA
Ouyang, B., Pan, N., Zhang, H., Xing, C., Ji, W."miR‑146b‑5p inhibits tumorigenesis and metastasis of gallbladder cancer by targeting Toll‑like receptor 4 via the nuclear factor‑κB pathway". Oncology Reports 45.4 (2021): 15.
Chicago
Ouyang, B., Pan, N., Zhang, H., Xing, C., Ji, W."miR‑146b‑5p inhibits tumorigenesis and metastasis of gallbladder cancer by targeting Toll‑like receptor 4 via the nuclear factor‑κB pathway". Oncology Reports 45, no. 4 (2021): 15. https://doi.org/10.3892/or.2021.7966