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Lycorine inhibits cell proliferation, migration and invasion, and primarily exerts in vitro cytostatic effects in human colorectal cancer via activating the ROS/p38 and AKT signaling pathways

  • Authors:
    • Ping Zhang
    • Xiaohui Yuan
    • Tingting Yu
    • Huakun Huang
    • Chunmei Yang
    • Lulu Zhang
    • Shengdong Yang
    • Xiaoji Luo
    • Jinyong Luo

  • View Affiliations

  • Published online on: February 5, 2021     https://doi.org/10.3892/or.2021.7970
  • Article Number: 19

  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Colorectal cancer (CRC) is a life‑threatening malignant tumor of the digestive tract. Diverse gene mutations and complicated alterations to the signaling pathways in CRC lead to heterogeneity in response to chemotherapy. Moreover, anticancer drugs for CRC chemotherapy are limited due to adverse events. Therefore, developing more effective, tolerable and safe drugs for the treatment of CRC is important. The present study aimed to investigate the effect of lycorine on human CRC cell proliferation, migration, invasion, apoptosis, cell cycle distribution, as well as the underlying molecular mechanism. The crystal violet staining and MTT assay results demonstrated that lycorine suppressed cell proliferation in a dose‑ and time‑dependent manner in the three CRC cell lines, HCT116, LoVo and SW480. Similarly, verified by performing wound healing and Transwell assays, lycorine significantly inhibited HCT116 and LoVo cell migration and invasion in vitro compared with the control group. In LoVo cells, the protein expression levels of matrix metallopeptidases, snail family transcriptional repressor 1, Vimentin and N‑cadherin were significantly downregulated, whereas the protein expression levels of E‑cadherin were significantly upregulated by lycorine treatment compared with the control group. The Hoechst 33258 staining and flow cytometry assay results indicated that lycorine mediated its cytostatic effect on CRC cells potentially via inducing cell cycle arrest, but not apoptosis. Compared with the control group, lycorine significantly induced HCT116 cell cycle arrest at the G2/M phase, but significantly induced LoVo cell cycle arrest at the S and G2/M phases. Furthermore, lycorine significantly downregulated the protein expression levels of cyclin D1 and cyclin E1, but significantly increased p21 and Smad4 protein expression levels in HCT116 and LoVo cells compared with the control group. The intracellular reactive oxygen species (ROS) measurement results also indicated that compared with the control group, lycorine significantly induced ROS accumulation, and increased phosphorylated‑p38 expression levels and AKT phosphorylation. Collectively, the present study suggested that lycorine might induce cell cycle arrest and exert cytostatic effects potentially via activating ROS/p38 and AKT signaling pathways in CRC cells.
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April-2021
Volume 45 Issue 4

Print ISSN: 1021-335X
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Spandidos Publications style
Zhang P, Yuan X, Yu T, Huang H, Yang C, Zhang L, Yang S, Luo X and Luo J: Lycorine inhibits cell proliferation, migration and invasion, and primarily exerts <em>in vitro</em> cytostatic effects in human colorectal cancer via activating the ROS/p38 and AKT signaling pathways. Oncol Rep 45: 19, 2021
APA
Zhang, P., Yuan, X., Yu, T., Huang, H., Yang, C., Zhang, L. ... Luo, J. (2021). Lycorine inhibits cell proliferation, migration and invasion, and primarily exerts <em>in vitro</em> cytostatic effects in human colorectal cancer via activating the ROS/p38 and AKT signaling pathways. Oncology Reports, 45, 19. https://doi.org/10.3892/or.2021.7970
MLA
Zhang, P., Yuan, X., Yu, T., Huang, H., Yang, C., Zhang, L., Yang, S., Luo, X., Luo, J."Lycorine inhibits cell proliferation, migration and invasion, and primarily exerts <em>in vitro</em> cytostatic effects in human colorectal cancer via activating the ROS/p38 and AKT signaling pathways". Oncology Reports 45.4 (2021): 19.
Chicago
Zhang, P., Yuan, X., Yu, T., Huang, H., Yang, C., Zhang, L., Yang, S., Luo, X., Luo, J."Lycorine inhibits cell proliferation, migration and invasion, and primarily exerts <em>in vitro</em> cytostatic effects in human colorectal cancer via activating the ROS/p38 and AKT signaling pathways". Oncology Reports 45, no. 4 (2021): 19. https://doi.org/10.3892/or.2021.7970