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Article Open Access

FOXD3‑AS1/miR‑128‑3p/LIMK1 axis regulates cervical cancer progression

  • Authors:
    • Xiufang Yang
    • Huilan Du
    • Wenhui Bian
    • Qingxue Li
    • Hairu Sun
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology, Hengshui People's Hospital, Hengshui, Hebei 053000, P.R. China, Department of Gynecology, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050000, P.R. China, Department of Gynecology, Chinese Medicine Hospital of Hebei, Shijiazhuang, Hebei 050000, P.R. China, Department of Gynecology, The Fourth Hospital of Shijiazhuang, Shijiazhuang, Hebei 050000, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 62
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    Published online on: March 16, 2021
       https://doi.org/10.3892/or.2021.8013
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Abstract

Long non‑coding RNA forkhead box D3 antisense RNA 1 (FOXD3‑AS1) functions as an oncogenic regulator in several types of cancer, including breast cancer, glioma and cervical cancer. However, the effects and mechanisms underlying FOXD3‑AS1 in cervical cancer (CC) are not completely understood. The present study aimed to investigate the biological functions and potential molecular mechanisms underlying FOXD3‑AS1 in CC progression. Reverse transcription‑quantitative PCR was performed to detect FOXD3‑AS1, microRNA (miR)‑128‑3p and LIM domain kinase 1 (LIMK1) expression levels in CC tissues and cells. Immunohistochemical staining and western blotting were conducted to assess LIMK1 protein expression levels in CC tissues and cells, respectively. Cell Counting Kit‑8 and BrdU assays were used to determine the role of FOXD3‑AS1 in regulating cell proliferation. CC cell migration and invasion were assessed by performing Transwell assays. Dual‑luciferase reporter assays were conducted to verify the binding between miR‑128‑3p and FOXD3‑AS1. FOXD3‑AS1 expression was significantly increased in CC tissues and cell lines compared with adjacent healthy tissues and normal cervical epithelial cells, respectively. High FOXD3‑AS1 expression was significantly associated with poor differentiation of tumor tissues, increased tumor size and positive lymph node metastasis. FOXD3‑AS1 overexpression significantly increased CC cell proliferation, migration and invasion compared with the negative control (NC) group, whereas FOXD3‑AS1 knockdown resulted in the opposite effects compared with the small interfering RNA‑NC group. Moreover, the results demonstrated that FOXD3‑AS1 targeted and negatively regulated miR‑128‑3p, which indirectly upregulated LIMK1 expression. Therefore, the present study demonstrated that FOXD3‑AS1 upregulated LIMK1 expression via competitively sponging miR‑128‑3p in CC cells, promoting CC progression.
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Copy and paste a formatted citation
Spandidos Publications style
Yang X, Du H, Bian W, Li Q and Sun H: FOXD3‑AS1/miR‑128‑3p/LIMK1 axis regulates cervical cancer progression. Oncol Rep 45: 62, 2021.
APA
Yang, X., Du, H., Bian, W., Li, Q., & Sun, H. (2021). FOXD3‑AS1/miR‑128‑3p/LIMK1 axis regulates cervical cancer progression. Oncology Reports, 45, 62. https://doi.org/10.3892/or.2021.8013
MLA
Yang, X., Du, H., Bian, W., Li, Q., Sun, H."FOXD3‑AS1/miR‑128‑3p/LIMK1 axis regulates cervical cancer progression". Oncology Reports 45.5 (2021): 62.
Chicago
Yang, X., Du, H., Bian, W., Li, Q., Sun, H."FOXD3‑AS1/miR‑128‑3p/LIMK1 axis regulates cervical cancer progression". Oncology Reports 45, no. 5 (2021): 62. https://doi.org/10.3892/or.2021.8013
Copy and paste a formatted citation
x
Spandidos Publications style
Yang X, Du H, Bian W, Li Q and Sun H: FOXD3‑AS1/miR‑128‑3p/LIMK1 axis regulates cervical cancer progression. Oncol Rep 45: 62, 2021.
APA
Yang, X., Du, H., Bian, W., Li, Q., & Sun, H. (2021). FOXD3‑AS1/miR‑128‑3p/LIMK1 axis regulates cervical cancer progression. Oncology Reports, 45, 62. https://doi.org/10.3892/or.2021.8013
MLA
Yang, X., Du, H., Bian, W., Li, Q., Sun, H."FOXD3‑AS1/miR‑128‑3p/LIMK1 axis regulates cervical cancer progression". Oncology Reports 45.5 (2021): 62.
Chicago
Yang, X., Du, H., Bian, W., Li, Q., Sun, H."FOXD3‑AS1/miR‑128‑3p/LIMK1 axis regulates cervical cancer progression". Oncology Reports 45, no. 5 (2021): 62. https://doi.org/10.3892/or.2021.8013
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