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Galectin‑9 suppresses the tumor growth of colon cancer in vitro and in vivo

  • Authors:
    • Asahiro Morishita
    • Kei Nomura
    • Joji Tani
    • Koji Fujita
    • Hisakazu Iwama
    • Kei Takuma
    • Mai Nakahara
    • Tomoko Tadokoro
    • Kyoko Oura
    • Taiga Chiyo
    • Shintaro Fujihara
    • Toshiro Niki
    • Mitsuomi Hirashima
    • Akira Nishiyama
    • Takashi Himoto
    • Tsutomu Masaki
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology and Neurology, Kagawa University, Faculty of Medicine, Miki‑cho, Kita‑gun, Kagawa 761‑0793, Japan, Life Science Research Center, Kagawa University, Faculty of Medicine, Miki‑cho, Kita‑gun, Kagawa 761‑0793, Japan, Department of Immunology and Immunopathology, Kagawa University, Faculty of Medicine, Miki‑cho, Kita‑gun, Kagawa 761‑0793, Japan, Department of Pharmacology, Kagawa University, Faculty of Medicine, Miki‑cho, Kita‑gun, Kagawa 761‑0793, Japan, Department of Medical Technology, Kagawa Prefectural University of Health Sciences, Mure‑cho, Takamatsu, Kagawa 761‑0123, Japan
    Copyright: © Morishita et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 105
    |
    Published online on: April 16, 2021
       https://doi.org/10.3892/or.2021.8056
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Abstract

Colon cancer is the second leading cause of cancer‑related mortality worldwide, and the prognosis of advanced colon cancer has remained poor in recent years. Galectin‑9 (Gal‑9) is a tandem‑repeat type galectin that has recently been shown to exert antiproliferative effects on various types of cancer cells. The present study aimed to assess the effects of Gal‑9 on human colon and colorectal cancer cells in vitro and in vivo, as well as to evaluate the microRNAs (miRNAs/miRs) associated with the antitumor effects of Gal‑9. We examined the ability of Gal‑9 to inhibit cell proliferation via apoptosis, and the effects of Gal‑9 on cell cycle‑related molecules in various human colon and colorectal cancer cell lines. In addition, Gal‑9‑mediated changes in activated tyrosine kinase receptors and angiogenic molecules were assessed using protein array chips in colon and colorectal cancer cells. Moreover, miRNA array analysis was performed to examine Gal‑9‑induced miRNA expression profiles. We also elucidated if Gal‑9 inhibited tumor growth in a murine in vivo model. We found that Gal‑9 suppressed the cell proliferation of colon cancer cell lines in vitro and in vivo. Our data further revealed that Gal‑9 increased caspase‑cleaved keratin 18 levels in Gal‑9‑treated colon cancer cells. In addition, Gal‑9 enhanced the phosphorylation of ALK, DDR1, and EphA10 proteins. Furthermore, the miRNA expression levels, such as miR‑1246, miR‑15b‑5p, and miR‑1237, were markedly altered by Gal‑9 treatment in vitro and in vivo. In conclusion, Gal‑9 suppresses the cell proliferation of human colon cancer by inducing apoptosis, and these findings suggest that Gal‑9 can be a potential therapeutic target in the treatment of colon cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Morishita A, Nomura K, Tani J, Fujita K, Iwama H, Takuma K, Nakahara M, Tadokoro T, Oura K, Chiyo T, Chiyo T, et al: Galectin‑9 suppresses the tumor growth of colon cancer <em>in vitro</em> and <em>in vivo</em>. Oncol Rep 45: 105, 2021.
APA
Morishita, A., Nomura, K., Tani, J., Fujita, K., Iwama, H., Takuma, K. ... Masaki, T. (2021). Galectin‑9 suppresses the tumor growth of colon cancer <em>in vitro</em> and <em>in vivo</em>. Oncology Reports, 45, 105. https://doi.org/10.3892/or.2021.8056
MLA
Morishita, A., Nomura, K., Tani, J., Fujita, K., Iwama, H., Takuma, K., Nakahara, M., Tadokoro, T., Oura, K., Chiyo, T., Fujihara, S., Niki, T., Hirashima, M., Nishiyama, A., Himoto, T., Masaki, T."Galectin‑9 suppresses the tumor growth of colon cancer <em>in vitro</em> and <em>in vivo</em>". Oncology Reports 45.6 (2021): 105.
Chicago
Morishita, A., Nomura, K., Tani, J., Fujita, K., Iwama, H., Takuma, K., Nakahara, M., Tadokoro, T., Oura, K., Chiyo, T., Fujihara, S., Niki, T., Hirashima, M., Nishiyama, A., Himoto, T., Masaki, T."Galectin‑9 suppresses the tumor growth of colon cancer <em>in vitro</em> and <em>in vivo</em>". Oncology Reports 45, no. 6 (2021): 105. https://doi.org/10.3892/or.2021.8056
Copy and paste a formatted citation
x
Spandidos Publications style
Morishita A, Nomura K, Tani J, Fujita K, Iwama H, Takuma K, Nakahara M, Tadokoro T, Oura K, Chiyo T, Chiyo T, et al: Galectin‑9 suppresses the tumor growth of colon cancer <em>in vitro</em> and <em>in vivo</em>. Oncol Rep 45: 105, 2021.
APA
Morishita, A., Nomura, K., Tani, J., Fujita, K., Iwama, H., Takuma, K. ... Masaki, T. (2021). Galectin‑9 suppresses the tumor growth of colon cancer <em>in vitro</em> and <em>in vivo</em>. Oncology Reports, 45, 105. https://doi.org/10.3892/or.2021.8056
MLA
Morishita, A., Nomura, K., Tani, J., Fujita, K., Iwama, H., Takuma, K., Nakahara, M., Tadokoro, T., Oura, K., Chiyo, T., Fujihara, S., Niki, T., Hirashima, M., Nishiyama, A., Himoto, T., Masaki, T."Galectin‑9 suppresses the tumor growth of colon cancer <em>in vitro</em> and <em>in vivo</em>". Oncology Reports 45.6 (2021): 105.
Chicago
Morishita, A., Nomura, K., Tani, J., Fujita, K., Iwama, H., Takuma, K., Nakahara, M., Tadokoro, T., Oura, K., Chiyo, T., Fujihara, S., Niki, T., Hirashima, M., Nishiyama, A., Himoto, T., Masaki, T."Galectin‑9 suppresses the tumor growth of colon cancer <em>in vitro</em> and <em>in vivo</em>". Oncology Reports 45, no. 6 (2021): 105. https://doi.org/10.3892/or.2021.8056
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