Open Access

Next‑generation sequencing analysis reveals that MTH‑3, a novel curcuminoid derivative, suppresses the invasion of MDA‑MB‑231 triple‑negative breast adenocarcinoma cells

  • Authors:
    • Yu-Jen Chiu
    • Fuu-Jen Tsai
    • Da-Tian Bau
    • Ling-Chu Chang
    • Min-Tsang Hsieh
    • Chi-Cheng Lu
    • Sheng-Chu Kuo
    • Jai-Sing Yang
  • View Affiliations

  • Published online on: May 19, 2021     https://doi.org/10.3892/or.2021.8084
  • Article Number: 133
  • Copyright: © Chiu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Triple‑negative breast cancer (TNBC) behaves aggressively in the invasive and metastatic states. Our research group recently developed a novel curcumin derivative, (1E,3Z,6E)-3-hydroxy-5-oxohepta-1,3,6-triene-1,7-diyl)bis(2‑methoxy-4,1‑phenylene)bis(3-hydroxy2-hydroxymethyl)-2‑methyl propanoate (MTH‑3), and previous studies showed that MTH‑3 inhibits TNBC proliferation and induces apoptosis in vitro and in vivo with a superior bioavailability and absorption than curcumin. In the present study, the effects of MTH‑3 on TNBC cell invasion were examined using various assays and gelatin zymography, and western blot analysis. Treatment with MTH‑3 inhibited MDA‑MB‑231 cell invasion and migration, as shown by Transwell assay, 3D spheroid invasion assay, and wound healing assay. The results of the gelatin zymography experiments revealed that MTH‑3 decreased matrix metalloproteinase‑9 activity. The potential signaling pathways were revealed by next‑generation sequencing analysis, antibody microarray analysis and western blot analysis. In conclusion, the results of the present study show that, MTH‑3 inhibited tumor cell invasion through the MAPK/ERK/AKT signaling pathway and cell cycle regulatory cascade, providing significant information about the potential molecular mechanisms of the effects of MTH‑3 on TNBC.
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July-2021
Volume 46 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Chiu Y, Tsai F, Bau D, Chang L, Hsieh M, Lu C, Kuo S and Yang J: Next‑generation sequencing analysis reveals that MTH‑3, a novel curcuminoid derivative, suppresses the invasion of MDA‑MB‑231 triple‑negative breast adenocarcinoma cells. Oncol Rep 46: 133, 2021
APA
Chiu, Y., Tsai, F., Bau, D., Chang, L., Hsieh, M., Lu, C. ... Yang, J. (2021). Next‑generation sequencing analysis reveals that MTH‑3, a novel curcuminoid derivative, suppresses the invasion of MDA‑MB‑231 triple‑negative breast adenocarcinoma cells. Oncology Reports, 46, 133. https://doi.org/10.3892/or.2021.8084
MLA
Chiu, Y., Tsai, F., Bau, D., Chang, L., Hsieh, M., Lu, C., Kuo, S., Yang, J."Next‑generation sequencing analysis reveals that MTH‑3, a novel curcuminoid derivative, suppresses the invasion of MDA‑MB‑231 triple‑negative breast adenocarcinoma cells". Oncology Reports 46.1 (2021): 133.
Chicago
Chiu, Y., Tsai, F., Bau, D., Chang, L., Hsieh, M., Lu, C., Kuo, S., Yang, J."Next‑generation sequencing analysis reveals that MTH‑3, a novel curcuminoid derivative, suppresses the invasion of MDA‑MB‑231 triple‑negative breast adenocarcinoma cells". Oncology Reports 46, no. 1 (2021): 133. https://doi.org/10.3892/or.2021.8084