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Cep63 knockout inhibits the malignant phenotypes of papillary thyroid cancer cell line TPC‑1

  • Authors:
    • Chenguang Liu
    • Fangqin Yu
    • Runsheng Ma
    • Lele Zhang
    • Gongbo Du
    • Dongpeng Niu
    • Detao Yin
  • View Affiliations / Copyright

    Affiliations: Department of Thyroid Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 199
    |
    Published online on: July 21, 2021
       https://doi.org/10.3892/or.2021.8150
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Abstract

The present study was designed to observe the expression of the centrosomal protein 63 in papillary thyroid cancer (PTC) tissues and cells and to explore the clinical significance of Cep63 expression in PTC. Primary PTC tissues and matched normal thyroid tissues were collected, and the Cep63 expression level was determined by reverse transcription‑quantitative PCR and western blotting. A stable Cep63‑knockout cell line was constructed to assess the proliferation, invasion, migration and apoptosis abilities in vitro. A subcutaneous tumorigenesis model was established in nude mice to evaluate the effect of Cep63 on tumor growth and proliferation in vivo. Western blotting was used to explore the relevant signaling pathways. The results revealed that the expression level of Cep63 in PTC tissues was significantly increased. The proliferation, invasion and migration abilities of TPC‑1 cells were decreased after Cep63 knockout, and silencing of Cep63 resulted in TPC‑1 cell cycle arrest in the S phase. Mechanistically, Cep63 knockout inhibited the activation of the Janus kinase/signal transducer and activator of transcription 3 signaling pathway. In conclusion, Cep63 knockout significantly inhibited biological functions of TPC‑1 cells in vitro and in vivo, indicating that Cep63 may be an important oncogene of PTC.
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1 

Wang S, Wu J, Ren J, Vlantis AC, Li MY, Liu SYW, Ng EKW, Chan ABW, Luo DC, Liu Z, et al: MicroRNA-125b interacts with Foxp3 to induce autophagy in thyroid cancer. Mol Ther. 26:2295–2303. 2018. View Article : Google Scholar : PubMed/NCBI

2 

Yin D, Wu W, Li M, Wang QE, Li H, Wang Y, Tang Y and Xing M: DKK3 is a potential tumor suppressor gene in papillary thyroid carcinoma. Endocr Relat Cancer. 20:507–514. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Dalal V, Kaur M and Bansal A: Papillary carcinoma thyroid with anastomosing channels: An unusual morphology. J Lab Physicians. 9:140–142. 2017. View Article : Google Scholar : PubMed/NCBI

4 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2017. CA Cancer J Clin. 67:7–30. 2017. View Article : Google Scholar : PubMed/NCBI

5 

Schneider DF and Chen H: New developments in the diagnosis and treatment of thyroid cancer. CA Cancer J Clin. 63:374–394. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Kim DH, Ahn JS, Han HJ, Kim HM, Hwang J, Lee KH, Cha-Molstad H, Ryoo IJ, Jang JH, Ko SK, et al: Cep131 overexpression promotes centrosome amplification and colon cancer progression by regulating Plk4 stability. Cell Death Dis. 10:5702019. View Article : Google Scholar : PubMed/NCBI

7 

Fukasawa K: Oncogenes and tumour suppressors take on centrosomes. Nat Rev Cancer. 7:911–924. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Brinkley BR: Managing the centrosome numbers game: From chaos to stability in cancer cell division. Trends Cell Biol. 11:18–21. 2001. View Article : Google Scholar : PubMed/NCBI

9 

Levine MS, Bakker B, Boeckx B, Moyett J, Lu J, Vitre B, Spierings DC, Lansdorp PM, Cleveland DW, Lambrechts D, et al: Centrosome amplification is sufficient to promote spontaneous tumorigenesis in mammals. Dev Cell. 40:313–322.e5. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Coelho PA, Bury L, Shahbazi MN, Liakath-Ali K, Tate PH, Wormald S, Hindley CJ, Huch M, Archer J, Skarnes WC, et al: Over-expression of Plk4 induces centrosome amplification, loss of primary cilia and associated tissue hyperplasia in the mouse. Open Biol. 5:1502092015. View Article : Google Scholar : PubMed/NCBI

11 

Maric I, Viaggi S, Caria P, Frau DV, Degan P and Vanni R: Centrosomal and mitotic abnormalities in cell lines derived from papillary thyroid cancer harboring specific gene alterations. Mol Cytogenet. 4:262011. View Article : Google Scholar : PubMed/NCBI

12 

Pannu V, Mittal K, Cantuaria G, Reid MD, Li X, Donthamsetty S, McBride M, Klimov S, Osan R, Gupta MV, et al: Rampant centrosome amplification underlies more aggressive disease course of triple negative breast cancers. Oncotarget. 6:10487–10497. 2015. View Article : Google Scholar : PubMed/NCBI

13 

Denu RA, Zasadil LM, Kanugh C, Laffin J, Weaver BA and Burkard ME: Centrosome amplification induces high grade features and is prognostic of worse outcomes in breast cancer. BMC Cancer. 16:472016. View Article : Google Scholar : PubMed/NCBI

14 

Kawamura K, Izumi H, Ma Z, Ikeda R, Moriyama M, Tanaka T, Nojima T, Levin LS, Fujikawa-Yamamoto K, Suzuki K and Fukasawa K: Induction of centrosome amplification and chromosome instability in human bladder cancer cells by p53 mutation and cyclin E overexpression. Cancer Res. 64:4800–4809. 2004. View Article : Google Scholar : PubMed/NCBI

15 

Xie S, Qin J, Liu S, Zhang Y, Wang J, Shi X, Li D, Zhou J and Liu M: Cep70 overexpression stimulates pancreatic cancer by inducing centrosome abnormality and microtubule disorganization. Sci Rep. 6:212632016. View Article : Google Scholar : PubMed/NCBI

16 

Li J, Xuan JW, Khatamianfar V, Valiyeva F, Moussa M, Sadek A, Yang BB, Dong BJ, Huang YR and Gao WQ: SKA1 over-expression promotes centriole over-duplication, centrosome amplification and prostate tumourigenesis. J Pathol. 234:178–189. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Nigg EA, Cajanek L and Arquint C: The centrosome duplication cycle in health and disease. FEBS Lett. 588:2366–2372. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Watanabe Y, Honda S, Konishi A, Arakawa S, Murohashi M, Yamaguchi H, Torii S, Tanabe M, Tanaka S, Warabi E and Shimizu S: Autophagy controls centrosome number by degrading Cep63. Nat Commun. 7:135082016. View Article : Google Scholar : PubMed/NCBI

19 

Buim ME, Soares FA, Sarkis AS and Nagai MA: The transcripts of SFRP1,CEP63 and EIF4G2 genes are frequently downregulated in transitional cell carcinomas of the bladder. Oncology. 69:445–454. 2005. View Article : Google Scholar : PubMed/NCBI

20 

Smith E, Dejsuphong D, Balestrini A, Hampel M, Lenz C, Takeda S, Vindigni A and Costanzo V: An ATM- and ATR-dependent checkpoint inactivates spindle assembly by targeting CEP63. Nat Cell Biol. 11:278–285. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Loffler H, Fechter A, Matuszewska M, Saffrich R, Mistrik M, Marhold J, Hornung C, Westermann F, Bartek J and Krämer A: Cep63 recruits Cdk1 to the centrosome: Implications for regulation of mitotic entry, centrosome amplification, and genome maintenance. Cancer Res. 71:2129–2139. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

23 

Zhi J, Wu Y, Hu L, Zhao J, Liu H, Ruan X, Hou X, Zhang J, Zheng X and Gao M: Assessment of the prognostic value and N1b changes of the eighth TNM/AJCC staging system for differentiated thyroid carcinoma. Int J Clin Oncol. 25:59–66. 2020. View Article : Google Scholar : PubMed/NCBI

24 

Kim TH, Kim YN, Kim HI, Park SY, Choe JH, Kim JH, Kim JS, Oh YL, Hahn SY, Shin JH, et al: Prognostic value of the eighth edition AJCC TNM classification for differentiated thyroid carcinoma. Oral Oncol. 71:81–86. 2017. View Article : Google Scholar : PubMed/NCBI

25 

Li S, Hu X, Cui S and He D: Novel centrosome protein, TCC52, is a cancer-testis antigen. Cancer Sci. 99:2274–2279. 2008. View Article : Google Scholar : PubMed/NCBI

26 

Kumar A, Rajendran V, Sethumadhavan R and Purohit R: CEP proteins: The knights of centrosome dynasty. Protoplasma. 250:965–983. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Yin DT, Xu J, Lei M, Li H, Wang Y, Liu Z, Zhou Y and Xing M: Characterization of the novel tumor-suppressor gene CCDC67 in papillary thyroid carcinoma. Oncotarget. 7:5830–5841. 2016. View Article : Google Scholar : PubMed/NCBI

28 

Liang C, Zhao T, Li H, He F, Zhao X, Zhang Y, Chu X, Hua C, Qu Y, Duan Y, et al: Long Non-coding RNA ITIH4-AS1 accelerates the proliferation and metastasis of colorectal cancer by activating JAK/STAT3 signaling. Mol Ther Nucleic Acids. 18:183–193. 2019. View Article : Google Scholar : PubMed/NCBI

29 

Khanna P, Lee JS, Sereemaspun A, Lee H and Baeg GH: GRAMD1B regulates cell migration in breast cancer cells through JAK/STAT and Akt signaling. Sci Rep. 8:95112018. View Article : Google Scholar : PubMed/NCBI

30 

Yu H, Lee H, Herrmann A, Buettner R and Jove R: Revisiting STAT3 signalling in cancer: New and unexpected biological functions. Nat Rev Cancer. 14:736–746. 2014. View Article : Google Scholar : PubMed/NCBI

31 

Notarangelo T, Sisinni L, Trino S, Calice G, Simeon V and Landriscina M: IL6/STAT3 axis mediates resistance to BRAF inhibitors in thyroid carcinoma cells. Cancer Lett. 433:147–155. 2018. View Article : Google Scholar : PubMed/NCBI

32 

Chang Q, Bournazou E, Sansone P, Berishaj M, Gao SP, Daly L, Wels J, Theilen T, Granitto S, Zhang X, et al: The IL-6/JAK/Stat3 feed-forward loop drives tumorigenesis and metastasis. Neoplasia. 15:848–862. 2013. View Article : Google Scholar : PubMed/NCBI

33 

Bi CL, Zhang YQ, Li B, Guo M and Fu YL: MicroRNA-520a-3p suppresses epithelial-mesenchymal transition, invasion, and migration of papillary thyroid carcinoma cells via the JAK1-mediated JAK/STAT signaling pathway. J Cell Physiol. 234:4054–4067. 2019. View Article : Google Scholar : PubMed/NCBI

34 

Lu J, Zhang L, Zhou H, Du Z and Zhang G: Silencing of Girdin suppresses the malignant behavior of colorectal carcinoma cells. Oncol Rep. 40:887–894. 2018.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liu C, Yu F, Ma R, Zhang L, Du G, Niu D and Yin D: Cep63 knockout inhibits the malignant phenotypes of papillary thyroid cancer cell line TPC‑1. Oncol Rep 46: 199, 2021.
APA
Liu, C., Yu, F., Ma, R., Zhang, L., Du, G., Niu, D., & Yin, D. (2021). Cep63 knockout inhibits the malignant phenotypes of papillary thyroid cancer cell line TPC‑1. Oncology Reports, 46, 199. https://doi.org/10.3892/or.2021.8150
MLA
Liu, C., Yu, F., Ma, R., Zhang, L., Du, G., Niu, D., Yin, D."Cep63 knockout inhibits the malignant phenotypes of papillary thyroid cancer cell line TPC‑1". Oncology Reports 46.3 (2021): 199.
Chicago
Liu, C., Yu, F., Ma, R., Zhang, L., Du, G., Niu, D., Yin, D."Cep63 knockout inhibits the malignant phenotypes of papillary thyroid cancer cell line TPC‑1". Oncology Reports 46, no. 3 (2021): 199. https://doi.org/10.3892/or.2021.8150
Copy and paste a formatted citation
x
Spandidos Publications style
Liu C, Yu F, Ma R, Zhang L, Du G, Niu D and Yin D: Cep63 knockout inhibits the malignant phenotypes of papillary thyroid cancer cell line TPC‑1. Oncol Rep 46: 199, 2021.
APA
Liu, C., Yu, F., Ma, R., Zhang, L., Du, G., Niu, D., & Yin, D. (2021). Cep63 knockout inhibits the malignant phenotypes of papillary thyroid cancer cell line TPC‑1. Oncology Reports, 46, 199. https://doi.org/10.3892/or.2021.8150
MLA
Liu, C., Yu, F., Ma, R., Zhang, L., Du, G., Niu, D., Yin, D."Cep63 knockout inhibits the malignant phenotypes of papillary thyroid cancer cell line TPC‑1". Oncology Reports 46.3 (2021): 199.
Chicago
Liu, C., Yu, F., Ma, R., Zhang, L., Du, G., Niu, D., Yin, D."Cep63 knockout inhibits the malignant phenotypes of papillary thyroid cancer cell line TPC‑1". Oncology Reports 46, no. 3 (2021): 199. https://doi.org/10.3892/or.2021.8150
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