Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1021-335X Online ISSN: 1791-2431
Journal Cover
May-2025 Volume 53 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
May-2025 Volume 53 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML

  • Supplementary Files
    • Supplementary_Data1.pdf
    • Supplementary_Data2.pdf
Article Open Access

Thermal cycling‑hyperthermia sensitizes non‑small cell lung cancer A549 cells to EGFR tyrosine kinase inhibitor erlotinib

  • Authors:
    • Guan-Bo Lin
    • Wei-Ting Chen
    • Yu-Yi Kuo
    • Hsu-Hsiang Liu
    • You-Ming Chen
    • Shr-Jeng Leu
    • Chih-Yu Chao
  • View Affiliations

    Affiliations: Department of Physics, Laboratory for Medical Physics and Biomedical Engineering, National Taiwan University, Taipei 106319, Taiwan, R.O.C., Molecular Imaging Center, National Taiwan University College of Medicine, Taipei 100233, Taiwan, R.O.C., Department of Biotechnology and Laboratory Science in Medicine, National Yang Ming Chiao Tung University, Taipei 112304, Taiwan, R.O.C.
  • Published online on: March 31, 2025     https://doi.org/10.3892/or.2025.8891
  • Article Number: 58
  • Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Molecular targeted therapy has emerged as a mainstream treatment for non‑small cell lung cancer (NSCLC), the most common type of lung cancer and the leading cause of cancer‑related death in both men and women. Erlotinib (Erl), a targeted therapy inhibiting EGFR pathways, has shown notable response rate in NSCLC cells. However, limited efficacy of the treatment has been reported due to resistance among a proportion of patients with NSCLC. Therefore, sensitizers are required to potentiate the efficacy of Erl in NSCLC treatment. The present study proposed a novel thermal therapy, thermal cycling‑hyperthermia (TC‑HT), as a supplement to amplify the effects of Erl. It was demonstrated that TC‑HT reduced the half‑maximal inhibitory concentration of Erl to 0.5 µM and TC‑HT sensitized A549 NSCLC cells to Erl via the downstream EGFR signaling cascades. Furthermore, the combination treatment of Erl and TC‑HT induced G2/M cell cycle arrest and inhibition of cell proliferation and migration. In addition, by slightly raising the temperature of TC‑HT, TC‑HT treatment alone produced antineoplastic effects without damaging the normal IMR‑90 lung cells. The method presented in this study may be applicable to other combination therapies and could potentially act as a starter for anticancer treatments, with fewer side effects.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

World Health Organization, . WHO report on cancer: Setting priorities, investing wisely and providing care for all. World Health Organization; 2020

2 

Balani C, Goss G and Blumenschein G Jr: Recent clinical developments and rationale for combining targeted agents in non-small cell lung cancer (NSCLC). Cancer Treat Rev. 38:174–184. 2012.

3 

Imyanitov EN, Iyevleva AG and Levchenko EV: Molecular testing and targeted therapy for non-small cell lung cancer: Current status and perspectives. Crit Rev Oncol Hemat. 157:1031942021. View Article : Google Scholar : PubMed/NCBI

4 

Wieduwilt MJ and Moasser MM: The epidermal growth factor receptor family: Biology driving targeted therapeutics. Cell Mol Life Sci. 65:1566–1584. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Yewale C, Baradia D, Vhora I, Patil S and Misra A: Epidermal growth factor receptor targeting in cancer: A review of trends and strategies. Biomaterials. 34:8690–8707. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Wee P and Wang Z: Epidermal growth factor receptor cell proliferation signaling pathways. Cancers (Basel). 29:522017. View Article : Google Scholar

7 

Ciardiello F, De Vita F, Orditura M and Tortora G: The role of EGFR inhibitors in nonsmall cell lung cancer. Curr Opin Oncol. 16:130–135. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Gridelli C, Maione P, Bareschino MA, Schettino C, Sacco PC, Ambrosio R, Barbato V, Falanga M and Rossi A: Erlotinib in the treatment of non-small cell lung cancer: Current status and future developments. Anticancer Res. 30:1301–1310. 2010.PubMed/NCBI

9 

Lin Y, Wang X and Jin H: EGFR-TKI resistance in NSCLC patients: Mechanisms and strategies. Am J Cancer Res. 4:411–435. 2014.PubMed/NCBI

10 

Melosky B: Supportive care treatments for toxicities of anti-EGFR and other targeted agents. Curr Oncol. 19:59–63. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Zhu CQ, da Cunha Santos G, Ding K, Sakurada A, Cutz JC, Liu N, Zhang T, Marrano P, Whitehead M, Squire JA, et al: Role of KRAS and EGFR as biomarkers of response to erlotinib in National Cancer Institute of Canada Clinical Trials Group Study BR.21. J Clin Oncol. 26:4268–4275. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Calvo E and Baselga J: Ethnic differences in response to epidermal growth factor receptor tyrosine kinase inhibitors. J Clin Oncol. 24:2158–2163. 2006. View Article : Google Scholar : PubMed/NCBI

13 

Garassino MC, Martelli O, Broggini M, Farina G, Veronese S, Rulli E, Bianchi F, Bettini A, Longo F, Moscetti L, et al: Erlotinib versus docetaxel as second-line treatment of patients with advanced non-small-cell lung cancer and wild-type EGFR tumours (TAILOR): A randomised controlled trial. Lancet Oncol. 14:981–988. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Raimbourg J, Joalland MP, Cabart M, de Plater L, Bouquet F, Savina A, Decaudin D, Bennouna J, Vallette FM and Lalier L: Sensitization of EGFR wild-type non-small cell lung cancer cells to EGFR-tyrosine kinase inhibitor erlotinib. Mol Cancer Ther. 16:1634–1644. 2017. View Article : Google Scholar : PubMed/NCBI

15 

Li T, Ling YH, Goldman ID and Perez-Soler R: Schedule-dependent cytotoxic synergism of pemetrexed and erlotinib in human non-small cell lung cancer cells. Clin Cancer Res. 13:3413–3422. 2007. View Article : Google Scholar : PubMed/NCBI

16 

Almanric K, Marceau N, Cantin A and Bertin É: Risk factors for nephrotoxicity associated with cisplatin. Can J Hosp Pharm. 70:99–106. 2017. View Article : Google Scholar : PubMed/NCBI

17 

Oei AL, Vriend LE, Crezee J, Franken NA and Krawczyk PM: Effects of hyperthermia on DNA repair pathways: One treatment to inhibit them all. Radiat Oncol. 10:1652015. View Article : Google Scholar : PubMed/NCBI

18 

Kaur P, Hurwitz MD, Krishnan S and Asea A: Combined hyperthermia and radiotherapy for the treatment of cancer. Cancers (Basel). 3:3799–3823. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Kwon S, Jung S and Baek SH: Combination therapy of radiation and hyperthermia, focusing on the synergistic Anti-cancer effects and research trends. Antioxidants. 12:9242023. View Article : Google Scholar : PubMed/NCBI

20 

Yang WH, Xie J, Lai ZY, Yang MD, Zhang GH, Li Y, Mu JB and Xu J: Radiofrequency deep hyperthermia combined with chemotherapy in the treatment of advanced Non-small cell lung cancer. Chin Med J. 132:922–927. 2019. View Article : Google Scholar : PubMed/NCBI

21 

Beik J, Abed Z, Ghoreishi FS, Hosseini-Nami S, Mehrzadi S, Shakeri-Zadeh A and Kamrava SK: Nanotechnology in hyperthermia cancer therapy: From fundamental principles to advanced applications. J Control Release. 235:205–221. 2016. View Article : Google Scholar : PubMed/NCBI

22 

Chen WT, Sun YK, Lu CH and Chao CY: Thermal cycling as a novel thermal therapy to synergistically enhance the anticancer effect of propolis on PANC-1 cells. Int J Oncol. 55:617–628. 2019.PubMed/NCBI

23 

Lu CH, Chen WT, Hsieh CH, Kuo YY and Chao CY: Thermal cycling-hyperthermia in combination with polyphenols, epigallocatechin gallate and chlorogenic acid, exerts synergistic anticancer effect against human pancreatic cancer PANC-1 cells. PLoS One. 14:e02176762019. View Article : Google Scholar : PubMed/NCBI

24 

Kuo YY, Chen WT, Lin GB, Lu CH and Chao CY: Study on the effect of a triple cancer treatment of propolis, thermal cycling-hyperthermia, and low-intensity ultrasound on PANC-1 cells. Aging. 15:7496–7512. 2023.PubMed/NCBI

25 

Lu CH, Kuo YY, Lin GB, Chen WT and Chao CY: Application of non-invasive low-intensity pulsed electric field with thermal cycling-hyperthermia for synergistically enhanced anticancer effect of chlorogenic acid on PANC-1 cells. PLoS One. 15:e02221262020. View Article : Google Scholar : PubMed/NCBI

26 

Hsieh CH, Lu CH, Chen WT, Ma BL and Chao CY: Application of non-invasive low strength pulsed electric field to EGCG treatment synergistically enhanced the inhibition effect on PANC-1 cells. PLoS One. 12:e01888852017. View Article : Google Scholar : PubMed/NCBI

27 

Ruttanapattanakul J, Wikan N, Potikanond S and Nimlamool W: Combination of pinocembrin and epidermal growth factor enhances the proliferation and survival of human keratinocytes. Int J Mol Sci. 24:124502023. View Article : Google Scholar : PubMed/NCBI

28 

Zou Y, Ling YH, Sironi J, Schwartz EL, Perez-Soler R and Piperdi B: The autophagy inhibitor chloroquine overcomes the innate resistance of Wild-type EGFR Non-small-cell lung cancer cells to erlotinib. J Thorac Oncol. 8:693–702. 2013. View Article : Google Scholar : PubMed/NCBI

29 

Otahal A, Aydemir D, Tomasich E and Minichsdorfer C: Delineation of cell death mechanisms induced by synergistic effects of statins and erlotinib in non-small cell lung cancer cell (NSCLC) lines. Sci Rep. 10:9592020. View Article : Google Scholar : PubMed/NCBI

30 

Li YL, Hu X, Li QY, Wang F, Zhang B, Ding K, Tan BQ, Lin NM and Zhang C: Shikonin sensitizes wild type EGFR NSCLC cells to erlotinib and gefitinib therapy. Mol Med Rep. 18:3882–3890. 2018.PubMed/NCBI

31 

Howe GA, Xiao B, Zhao H, Al-Zahrani KN, Hasim MS, Villeneuve J, Sekhon HS, Goss GD, Sabourin LA, Dimitroulakos J and Addison CL: Focal adhesion kinase inhibitors in combination with erlotinib demonstrate enhanced Anti-tumor activity in Non-small cell lung cancer. PLoS One. 11:e01505672016. View Article : Google Scholar : PubMed/NCBI

32 

Greve G, Schiffmann I, Pfeifer D, Pantic M, Schüler J and Lübbert M: The pan-HDAC inhibitor panobinostat acts as a sensitizer for erlotinib activity in EGFR-mutated and -wildtype non-small cell lung cancer cells. BMC Cancer. 15:9472015. View Article : Google Scholar : PubMed/NCBI

33 

Atalay G, Cardoso F, Awada A and Piccart MJ: Novel therapeutic strategies targeting the epidermal growth factor receptor (EGFR) family and its downstream effectors in breast cancer. Ann Oncol. 14:1346–1363. 2003. View Article : Google Scholar : PubMed/NCBI

34 

Takeuchi K and Ito F: EGF receptor in relation to tumor development: Molecular basis of responsiveness of cancer cells to EGFR-targeting tyrosine kinase inhibitors. FEBS J. 277:316–326. 2010. View Article : Google Scholar : PubMed/NCBI

35 

Akca H, Tani M, Hishida T, Matsumoto S and Yokota J: Activation of the AKT and STAT3 pathways and prolonged survival by a mutant EGFR in human lung cancer cells. Lung Cancer. 54:25–33. 2006. View Article : Google Scholar : PubMed/NCBI

36 

Matsuyama S and Reed JC: Mitochondria-dependent apoptosis and cellular pH regulation. Cell Death Differ. 7:1155–1165. 2000. View Article : Google Scholar : PubMed/NCBI

37 

Brentnall M, Rodriguez-Menocal L, De Guevara RL, Cepero E and Boise LH: Caspase-9, caspase-3 and caspase-7 have distinct roles during intrinsic apoptosis. BMC Cell Biol. 14:322013. View Article : Google Scholar : PubMed/NCBI

38 

Yi M, Dong B, Qin S, Chu Q, Wu K and Luo S: Advances and perspectives of PARP inhibitors. Exp Hematol Oncol. 8:445732019. View Article : Google Scholar : PubMed/NCBI

39 

Gad H, Koolmeister T, Jemth AS, Eshtad S, Jacques SA, Ström CE, Svensson LM, Schultz N, Lundbäck T, Einarsdottir BO, et al: MTH1 inhibition eradicates cancer by preventing sanitation of the dNTP pool. Nature. 508:215–221. 2014. View Article : Google Scholar : PubMed/NCBI

40 

Li DN, Yang CC, Li J, Ou Yang QG, Zeng LT, Fan GQ, Liu TH, Tian XY, Wang JJ, Zhang H, et al: The high expression of MTH1 and NUDT5 promotes tumor metastasis and indicates a poor prognosis in patients with non-small-cell lung cancer. Biochim Biophys Acta Mol Cell Res. 1868:1188952021. View Article : Google Scholar : PubMed/NCBI

41 

Dorée M and Hunt T: From Cdc2 to Cdk1: When did the cell cycle kinase join its cyclin partner? J Cell Sci. 115:2461–2464. 2002. View Article : Google Scholar : PubMed/NCBI

42 

Lim S and Kaldis P: Cdks, cyclins and CKIs: Roles beyond cell cycle regulation. Development. 140:3079–3093. 2013. View Article : Google Scholar : PubMed/NCBI

43 

Chang CC, Heller JD, Kuo J and Huang RC: Tetra-O-methyl nordihydroguaiaretic acid induces growth arrest and cellular apoptosis by inhibiting Cdc2 and survivin expression. Proc Natl Acad USA Sci. 101:13239–13244. 2004. View Article : Google Scholar : PubMed/NCBI

44 

Senju M, Sueoka N, Sato A, Iwanaga K, Sakao Y, Tomimitsu S, Tominaga M, Irie K, Hayashi S and Sueoka E: Hsp90 inhibitors cause G2/M arrest associated with the reduction of Cdc25C and Cdc2 in lung cancer cell lines. J Cancer Res Clin Oncol. 132:150–158. 2006. View Article : Google Scholar : PubMed/NCBI

45 

Yoshida M, Matsui Y, Iizuka A and Ikarashi Y: G2-phase arrest through p21(WAF1/Cip1) induction and cdc2 repression by gnidimacrin in human hepatoma HLE cells. Anticancer Res. 29:1349–1354. 2009.PubMed/NCBI

46 

Su JC, Lin KL, Chien CM, Lu CM, Chen YL, Chang LS and Lin SR: Novel indoloquinoline derivative, IQDMA, induces G(2)/M phase arrest and apoptosis in A549 cells through JNK/p38 MAPK signaling activation. Life Sci. 85:505–516. 2009. View Article : Google Scholar : PubMed/NCBI

47 

Pai JT, Hsu MW, Leu YL, Chang KT and Weng MS: Induction of G2/M cell cycle arrest via p38/p21Waf1/Cip1-dependent signaling pathway activation by bavachinin in non-small-cell lung cancer cells. Molecules. 26:51612021. View Article : Google Scholar : PubMed/NCBI

48 

Luo YH, Wang C, Xu WT, Zhang Y, Zhang T, Xue H, Li YN, Fu ZR, Wang Y and Jin CH: 18β-Glycyrrhetinic acid has Anti-cancer effects via inducing apoptosis and G2/M cell cycle arrest, and inhibiting migration of A549 lung cancer cells. Onco Targets Ther. 14:5131–5144. 2021. View Article : Google Scholar : PubMed/NCBI

49 

Tamura T, Kurishima K, Nakazawa K, Kagohashi K, Ishikawa H, Satoh H and Hizawa N: Specific organ metastases and survival in metastatic non-small-cell lung cancer. Mol Clin Oncol. 3:217–221. 2015. View Article : Google Scholar : PubMed/NCBI

50 

Uramoto H and Tanaka F: Recurrence after surgery in patients with NSCLC. Transl Lung Cancer Res. 4:2422014.PubMed/NCBI

51 

Passaro A, Jänne PA, Mok T and Peters S: Overcoming therapy resistance in EGFR-mutant lung cancer. Nat Cancer. 2:377–391. 2021. View Article : Google Scholar : PubMed/NCBI

52 

Tong CW, Wu WK, Loong HH, Cho WC and To KK: Drug combination approach to overcome resistance to EGFR tyrosine kinase inhibitors in lung cancer. Cancer Lett. 405:100–110. 2017. View Article : Google Scholar : PubMed/NCBI

53 

Cavazzoni A, Alfieri RR, Cretella D, Saccani F, Ampollini L, Galetti M, Quaini F, Graiani G, Madeddu D, Mozzoni P, et al: Combined use of anti-ErbB monoclonal antibodies and erlotinib enhances antibody-dependent cellular cytotoxicity of wild-type erlotinib-sensitive NSCLC cell lines. Mol Cancer. 11:912012. View Article : Google Scholar : PubMed/NCBI

54 

Hu X, Wu LW, Weng X, Lin NM and Zhang C: Synergistic antitumor activity of aspirin and erlotinib: Inhibition of p38 enhanced aspirin plus Erlotinib-induced suppression of metastasis and promoted cancer cell apoptosis. Oncol Lett. 16:2715–2724. 2018.PubMed/NCBI

55 

Chen JC, Ko JC, Yen TC, Chen TY, Lin YC, Ma PF and Lin YW: Capsaicin enhances erlotinib-induced cytotoxicity via AKT inactivation and excision repair cross-complementary 1 (ERCC1) down-regulation in human lung cancer cells. Toxicol Res. 8:459–470. 2019. View Article : Google Scholar : PubMed/NCBI

56 

Bing C, Cheng B, Staruch RM, Nofiele J, Wodzak Staruch M, Szczepanski D, Farrow-Gillespie A, Yang A, Laetsch TW and Chopra R: Breath-hold MR-HIFU hyperthermia: Phantom and in vivo feasibility. Int J Hyperthermia. 36:1084–1097. 2019. View Article : Google Scholar : PubMed/NCBI

57 

Sadhukha T, Wiedmann TS and Panyam J: Inhalable magnetic nanoparticles for targeted hyperthermia in lung cancer therapy. Biomaterials. 34:5163–5171. 2013. View Article : Google Scholar : PubMed/NCBI

58 

Park J and Baek SH: Combination therapy with cinnamaldehyde and hyperthermia induces apoptosis of A549 Non-small cell lung carcinoma cells via regulation of reactive oxygen species and mitogen-Activated protein kinase family. Int J Mol Sci. 21:62292020. View Article : Google Scholar : PubMed/NCBI

59 

Heo J, Jo Y and Yoon M: Synergistic effects of combined hyperthermia and electric fields treatment in non-small cell lung-cancer (NSCLC) cell lines. Clin Transl Oncol. Oct 22–2024.(Epub ahead of print). doi: 10.1007/s12094-024-03760-6. View Article : Google Scholar : PubMed/NCBI

60 

Cheng H, An SJ, Dong S, Zhang YF, Zhang XC, Chen ZH, Jian-Su and Wu YL: Molecular mechanism of the schedule-dependent synergistic interaction in EGFR-mutant non-small cell lung cancer cell lines treated with paclitaxel and gefitinib. J Hematol Oncol. 4:52011. View Article : Google Scholar : PubMed/NCBI

61 

Volman Y, Hefetz R, Galun E and Rachmilewitz J: DNA damage alters EGFR signaling and reprograms cellular response via Mre-11. Sci Rep. 12:57602022. View Article : Google Scholar : PubMed/NCBI

62 

Feng YB, Chen L, Chen FX, Yang Y, Chen GH, Zhou ZH and Xu CF: Immunopotentiation effects of apigenin on NK cell proliferation and killing pancreatic cancer cells. Int J Immunopathol Pharmacol. 37:39463202311611742023. View Article : Google Scholar : PubMed/NCBI

63 

Xu J, Jiao W, Wu DB, Yu JH, Liu LJ, Zhang MY and Chen GX: Yishen Tongbi decoction attenuates inflammation and bone destruction in rheumatoid arthritis by regulating JAK/STAT3/SOCS3 pathway. Front Immunol. 15:13818022024. View Article : Google Scholar : PubMed/NCBI

64 

Steen NV, Potze L, Giovannetti E, Cavazzoni A, Ruijtenbeek R, Rolfo C, Pauwels P and Peters GJ: Molecular mechanism underlying the pharmacological interactions of the protein kinase C-β inhibitor enzastaurin and erlotinib in non-small cell lung cancer cells. Am J Cancer Res. 7:816–830. 2017.PubMed/NCBI

65 

Cheng F, Peng X, Meng G, Pu Y, Luo K and He B: Poly(ester-thioether) microspheres co-loaded with erlotinib and α-tocopheryl succinate for combinational therapy of non-small cell lung cancer. J Mater Chem B. 8:1728–1738. 2020. View Article : Google Scholar : PubMed/NCBI

66 

Wust P, Hildebrandt B, Sreenivasa G, Rau B, Gellermann J, Riess H, Felix R and Schlag PM: Hyperthermia in combined treatment of cancer. Lancet Oncol. 3:487–497. 2002. View Article : Google Scholar : PubMed/NCBI

67 

Belhadj Slimen I, Najar T, Ghram A, Dabbebi H, Ben Mrad M and Abdrabbah M: Reactive oxygen species, heat stress and oxidative-induced mitochondrial damage. A review. Int J Hyperthermia. 30:513–523. 2014. View Article : Google Scholar : PubMed/NCBI

68 

Panieri E and Santoro M: ROS homeostasis and metabolism: A dangerous liason in cancer cells. Cell Death Dis. 7:e22532016. View Article : Google Scholar : PubMed/NCBI

69 

Van der Zee J: Heating the patient: A promising approach? Ann Oncol. 13:1173–1184. 2002. View Article : Google Scholar : PubMed/NCBI

70 

Salem A, Asselin MC, Reymen B, Jackson A, Lambin P, West CML, O'Connor JPB and Faivre-Finn C: Targeting hypoxia to improve non-Small cell lung cancer outcome. J Natl Cancer Inst. 110:14–30. 2018. View Article : Google Scholar

71 

Gerweck LE, Nygaard TG and Burlett M: Response of cells to hyperthermia under acute and chronic hypoxic conditions. Cancer Res. 39:966–972. 1979.PubMed/NCBI

72 

Bicher HI, Hetzel FW, Sandhu TS, Frinak S, Vaupel P, O'Hara MD and O'Brien T: Effects of hyperthermia on normal and tumor microenvironment. Radiology. 137:523–530. 1980. View Article : Google Scholar : PubMed/NCBI

73 

Overgaard J: Effect of hyperthermia on the hypoxic fraction in an experimental mammary carcinoma in vivo. Br J Radiol. 54:245–249. 1981. View Article : Google Scholar : PubMed/NCBI

74 

Elming PB, Sørensen BS, Oei AL, Franken NAP, Crezee J, Overgaard J and Horsman MR: Hyperthermia: The optimal treatment to overcome radiation resistant hypoxia. Cancers. 11:602019. View Article : Google Scholar : PubMed/NCBI

75 

Kabakov AE and Yakimova AO: Hypoxia-induced cancer cell responses driving radioresistance of hypoxic tumors: Approaches to targeting and radiosensitizing. Cancers. 13:11022021. View Article : Google Scholar : PubMed/NCBI

76 

Hu C, Yang J, Qi Z, Wu H, Wang B, Zou F, Mei H, Liu J, Wang W and Liu Q: Heat shock proteins: Biological functions, pathological roles, and therapeutic opportunities. MedComm. 3:e1612022. View Article : Google Scholar : PubMed/NCBI

77 

Ahmed K, Zaidi SF, Mati-Ur-Rehman Rehman R and Kondo T: Hyperthermia and protein homeostasis: Cytoprotection and cell death. J Therm Biol. 91:1026152020. View Article : Google Scholar : PubMed/NCBI

78 

Scutigliani EM, Liang Y, Crezee H, Kanaar R and Krawczyk PM: Modulating the heat stress response to improve Hyperthermia-Based anticancer treatments. Cancers. 13:12432021. View Article : Google Scholar : PubMed/NCBI

79 

Karar J and Maity A: Modulating the tumor microenvironment to increase radiation responsiveness. Cancer Biol Ther. 8:1994–2001. 2009. View Article : Google Scholar : PubMed/NCBI

80 

Nijkamp MM, Span PN, Bussink J and Kaanders JH: Interaction of EGFR with the tumour microenvironment: Implications for radiation treatment. Radiother Oncol. 108:17–23. 2013. View Article : Google Scholar : PubMed/NCBI

81 

Zhang T, Chen L, Zhang S, Xu Y, Fan Y and Zhang L: Effects of high-intensity focused ultrasound on Cisplatin-resistant human lung adenocarcinoma in vitro and in vivo. Acta Biochim Biophys Sin. 49:1092–1098. 2017. View Article : Google Scholar : PubMed/NCBI

82 

Qin Y, Sun Y, Liu Y, Luo Y and Zhu J: Pilot study of radiofrequency hyperthermia in combination with gefitinib in gefitinib-effective patients with advanced NSCLC. Thorac Cancer. 7:422–427. 2016. View Article : Google Scholar : PubMed/NCBI

83 

Sekins KM, Leeper DB, Hoffman JK, Keilman GW, Ziskin MC, Wolfson MR and Shaffer TH: Feasibility of lung cancer hyperthermia using breathable perfluorochemical (PFC) liquids. Part II: Ultrasound hyperthermia. Int J Hyperthermia. 20:278–299. 2004. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Lin G, Chen W, Kuo Y, Liu H, Chen Y, Leu S and Chao C: Thermal cycling‑hyperthermia sensitizes non‑small cell lung cancer A549 cells to EGFR tyrosine kinase inhibitor erlotinib. Oncol Rep 53: 58, 2025.
APA
Lin, G., Chen, W., Kuo, Y., Liu, H., Chen, Y., Leu, S., & Chao, C. (2025). Thermal cycling‑hyperthermia sensitizes non‑small cell lung cancer A549 cells to EGFR tyrosine kinase inhibitor erlotinib. Oncology Reports, 53, 58. https://doi.org/10.3892/or.2025.8891
MLA
Lin, G., Chen, W., Kuo, Y., Liu, H., Chen, Y., Leu, S., Chao, C."Thermal cycling‑hyperthermia sensitizes non‑small cell lung cancer A549 cells to EGFR tyrosine kinase inhibitor erlotinib". Oncology Reports 53.5 (2025): 58.
Chicago
Lin, G., Chen, W., Kuo, Y., Liu, H., Chen, Y., Leu, S., Chao, C."Thermal cycling‑hyperthermia sensitizes non‑small cell lung cancer A549 cells to EGFR tyrosine kinase inhibitor erlotinib". Oncology Reports 53, no. 5 (2025): 58. https://doi.org/10.3892/or.2025.8891
Copy and paste a formatted citation
x
Spandidos Publications style
Lin G, Chen W, Kuo Y, Liu H, Chen Y, Leu S and Chao C: Thermal cycling‑hyperthermia sensitizes non‑small cell lung cancer A549 cells to EGFR tyrosine kinase inhibitor erlotinib. Oncol Rep 53: 58, 2025.
APA
Lin, G., Chen, W., Kuo, Y., Liu, H., Chen, Y., Leu, S., & Chao, C. (2025). Thermal cycling‑hyperthermia sensitizes non‑small cell lung cancer A549 cells to EGFR tyrosine kinase inhibitor erlotinib. Oncology Reports, 53, 58. https://doi.org/10.3892/or.2025.8891
MLA
Lin, G., Chen, W., Kuo, Y., Liu, H., Chen, Y., Leu, S., Chao, C."Thermal cycling‑hyperthermia sensitizes non‑small cell lung cancer A549 cells to EGFR tyrosine kinase inhibitor erlotinib". Oncology Reports 53.5 (2025): 58.
Chicago
Lin, G., Chen, W., Kuo, Y., Liu, H., Chen, Y., Leu, S., Chao, C."Thermal cycling‑hyperthermia sensitizes non‑small cell lung cancer A549 cells to EGFR tyrosine kinase inhibitor erlotinib". Oncology Reports 53, no. 5 (2025): 58. https://doi.org/10.3892/or.2025.8891
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team