Open Access

Analysis of human papilloma virus type 52 integration status in exfoliated cervical cells

  • Authors:
    • Ke Zhang
    • Zhanjun Liu
    • Ji Li
    • Juan Li
    • Jianghong Yan
    • Yunchuan Su
    • Shuying Li
    • Jintao Li
  • View Affiliations

  • Published online on: October 11, 2017     https://doi.org/10.3892/etm.2017.5279
  • Pages:5817-5824
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

To explore the significance of human papilloma virus type 52 (HPV52) infection and its integration in cells within cervical lesions, the expression levels of HPV52 were detected using polymerase chain reaction (PCR). The copy numbers of HPV52 E2, HPV52 E6 and the reference gene β‑actin were determined by quantitative PCR to analyze the association between HPV52 integration and cervical lesions. HPV52 integration was analyzed by the amplification of papillomavirus oncogene transcripts. A total of 13 samples from 468 cases were positive for HPV52. Among the samples, 1 case with an E2/E6 ratio >1 was purely episomal, 3 cases with an E2/E6 ratio of 0 were purely integrated, and 9 cases with an E2/E6 ratio of between 0 and 1 were a mixture of integrated and episomal. With the progression of cervical disease, the prevalence of the episomal type decreased gradually, and the prevalence of the integrated (episomal and integrated) forms increased. The pure integration of HPV52 occurred in chromosomes 2, 5 and 8. These results indicate that HPV52 integration into the host genome may be a key factor in cervical lesions. Thus, patients at high risk for cervical lesions may potentially be identified by screening for HPV52 infection and integration.

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December 2017
Volume 14 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
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APA
Zhang, K., Liu, Z., Li, J., Li, J., Yan, J., Su, Y. ... Li, J. (2017). Analysis of human papilloma virus type 52 integration status in exfoliated cervical cells. Experimental and Therapeutic Medicine, 14, 5817-5824. https://doi.org/10.3892/etm.2017.5279
MLA
Zhang, K., Liu, Z., Li, J., Li, J., Yan, J., Su, Y., Li, S., Li, J."Analysis of human papilloma virus type 52 integration status in exfoliated cervical cells". Experimental and Therapeutic Medicine 14.6 (2017): 5817-5824.
Chicago
Zhang, K., Liu, Z., Li, J., Li, J., Yan, J., Su, Y., Li, S., Li, J."Analysis of human papilloma virus type 52 integration status in exfoliated cervical cells". Experimental and Therapeutic Medicine 14, no. 6 (2017): 5817-5824. https://doi.org/10.3892/etm.2017.5279