Berberine inhibits the LPS‑induced proliferation and inflammatory response of stromal cells of adenomyosis tissues mediated by the LPS/TLR4 signaling pathway

  • Authors:
    • Li Liu
    • Li Chen
    • Caixia Jiang
    • Jing Guo
    • Yan Xie
    • Le Kang
    • Zhongping Cheng
  • View Affiliations

  • Published online on: October 16, 2017     https://doi.org/10.3892/etm.2017.5316
  • Pages:6125-6130
0

Abstract

A previous study by our group has demonstrated that lipopolysaccharide (LPS) induces adenomyosis through stimulating inflammatory cell proliferation and invasive growth of stromal cells via Toll‑like receptor 4 (TLR4) signaling. The present study aimed to investigate the effects of berberine (BBR) on LPS‑induced ectopic endometrial stromal cells (EESCs) isolated from patients with adenomyosis. The viability of EESCs treated with LPS or LPS plus BBR was detected by a cell counting kit‑8 assay, and the cell cycle distribution and apoptosis were evaluated by flow cytometry. The effect of BBR on the expression of key molecules of inflammatory proliferation and invasive growth of LPS‑induced EESCs was also evaluated. BBR significantly inhibited the LPS‑induced proliferation of EESCs in a dose‑ and time‑dependent manner. BBR induced cell cycle arrest in G0/G1 phase and enhanced apoptosis of LPS‑induced EESCs. Furthermore, BBR inhibited the expression of interleukin (IL)‑6, IL‑8, transforming growth factor‑β, epithelial growth factor, vascular endothelial growth factor and matrix metalloproteinase 2 in LPS‑induced EESCs. To the best of our knowledge, the present study was the first to demonstrate that BBR has a protective effect on ameliorating the LPS‑induced progression of adenomyosis. This result may provide a novel therapeutic strategy for the clinical treatment of the disease

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December 2017
Volume 14 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
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APA
Liu, L., Chen, L., Jiang, C., Guo, J., Xie, Y., Kang, L., & Cheng, Z. (2017). Berberine inhibits the LPS‑induced proliferation and inflammatory response of stromal cells of adenomyosis tissues mediated by the LPS/TLR4 signaling pathway. Experimental and Therapeutic Medicine, 14, 6125-6130. https://doi.org/10.3892/etm.2017.5316
MLA
Liu, L., Chen, L., Jiang, C., Guo, J., Xie, Y., Kang, L., Cheng, Z."Berberine inhibits the LPS‑induced proliferation and inflammatory response of stromal cells of adenomyosis tissues mediated by the LPS/TLR4 signaling pathway". Experimental and Therapeutic Medicine 14.6 (2017): 6125-6130.
Chicago
Liu, L., Chen, L., Jiang, C., Guo, J., Xie, Y., Kang, L., Cheng, Z."Berberine inhibits the LPS‑induced proliferation and inflammatory response of stromal cells of adenomyosis tissues mediated by the LPS/TLR4 signaling pathway". Experimental and Therapeutic Medicine 14, no. 6 (2017): 6125-6130. https://doi.org/10.3892/etm.2017.5316