Function of miR‑25 in the invasion and metastasis of esophageal squamous carcinoma cells and bioinformatical analysis of the miR-106b-25 cluster

Wang,Meng; Yang,Yangyang  Ou; Jin,Qingtao; Shang,Linlin; Zhang,Jian

doi:10.3892/etm.2017.5358

Function of miR‑25 in the invasion and metastasis of esophageal squamous carcinoma cells and bioinformatical analysis of the miR-106b-25 cluster

Authors:
- Meng Wang
- Yangyang Ou Yang
- Qingtao Jin
- Linlin Shang
- Jian Zhang
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Affiliations: Medical Department, Jining No. 1 People's Hospital, Jining, Shandong 272011, P.R. China, Department of Gastroenterology, Jining No. 1 People's Hospital, Jining, Shandong 272011, P.R. China, Department of Gastroenterology, Jiaxiang People's Hospital, Jining, Shandong 272400, P.R. China, Department of Pharmacy, Jining No. 2 People's Hospital, Jining, Shandong 272011, P.R. China
Published online on: October 23, 2017 https://doi.org/10.3892/etm.2017.5358
Pages: 440-446

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Abstract

MicroRNAs (miRNAs/miRs) are a class of small, non‑coding RNA molecules that serve a key function in carcinogenesis and tumor progression. Recent evidence indicates that miRNAs may act as powerful regulators of migration and invasion. The present study aimed to investigate the effect of miR‑25 on the invasion and metastasis of KYSE‑150 and EC109 esophageal squamous cell carcinoma (ESCC) cells, and predict the mechanism of this effect by bioinformatically analyzing the miR‑106b‑25 cluster. In order to alter the expression of miR‑25 in the two cell lines, a miR‑25 inhibitor or mimic were transfected into the cells, which were then studied via Transwell migration and invasion assays. Subsequently, the target genes of the miR‑106b‑25 cluster were predicted using miRanda, PicTar, TargetScan and miRTarbase, and the functions of the target genes were predicted via Gene Ontology term and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses. Then, a protein‑protein interaction (PPI) network was produced using the Search Tool for the Retrieval of Interacting Genes. The results revealed that overexpressing miR‑25 led to significantly increased cell migration and invasion in KYSE150 and EC109 cells. Suppressing miR‑25 resulted in significantly decreased cell migration and invasion in KYSE150 cells, while the result was not significant in EC109 cells. Target genes of the miR‑106b‑25 cluster were significantly enriched in the biological process regulation of cellular metabolic process and several cancer‑associated pathways, such as those for glioma and melanoma. The PPI network revealed that PTEN, TP53, MDM2, E2F1, PRMT5, MCM2, RB1, CDKN1A, SHAD7 and EZH2 may serve core roles within the network and associate with one another during the pathogenesis of ESCC. These results indicate that a high expression of miR‑25 promotes the invasion and metastasis of ESCC cells, while the influence of low expression of miR‑25 differs with cells with different degrees of differentiation. Invasion and metastasis are not effected in cells with poor differentiation, while they were decreased in well differentiated cells. Furthermore, PTEN, TP53, MDM2, E2F1, PRMT5, MCM2, RB1, CDKN1A, SHAD7 and EZH2 may be targeted by the miR‑106b‑25 cluster, and act together to regulate the development of ESCC.

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