Open Access

Evaluation of the effects of the combination of BMP‑2‑modified BMSCs and PRP on cartilage defects

  • Authors:
    • Shiqiang Ruan
    • Jiang Deng
    • Ling Yan
    • Wenliang Huang
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  • Published online on: September 19, 2018     https://doi.org/10.3892/etm.2018.6776
  • Pages: 4569-4577
  • Copyright: © Ruan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Articular cartilage is avascular and aneural, and has limited capacity for self‑regeneration when injured. Tissue engineering has emerged as a promising approach in repairing cartilage defects. To improve the therapy of cartilage healing, the present study investigated the efficacy of the combination of lentivirus‑mediated bone morphogenetic protein‑2 (BMP2) in bone marrow‑derived stromal cells (BMSCs) and platelet‑rich plasma (PRP) on cartilage and bone healing in a cartilage defect model using the rabbit knee. The BMSCs were harvested from New Zealand rabbits and transduced with lentivirus carrying BMP‑2. Standard bone defects were introduced in the femoral groove of patellofemoral joints of 48 New Zealand rabbits. The cartilage defects were subjected to synthetic scaffold mosaicplasty with chitosan/silk fibroin/nanohydroxyapatite particles tri‑component scaffolds soaked in BMSCs and PRP. After 16 weeks, the tissue specimens were assessed by micro‑computed tomography (micro‑CT) and macroscopic examination. The results showed that lentivirus‑mediated BMP‑2 and PRP increased the cell viability of the BMSCs, induced the expression of associated genes and enhanced osteogenic differentiation in vitro. In vivo, the expression of BMP‑2 was observed for 16 weeks. The combination of BMP‑2 and PRP treatment led to optimal results, compared with the other groups on micro‑CT and gross observations. The results of the present study present a novel therapy using the lentivirus‑mediated BMP‑2 gene together with PRP for cartilage healing.
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December-2018
Volume 16 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Ruan S, Deng J, Yan L and Huang W: Evaluation of the effects of the combination of BMP‑2‑modified BMSCs and PRP on cartilage defects. Exp Ther Med 16: 4569-4577, 2018
APA
Ruan, S., Deng, J., Yan, L., & Huang, W. (2018). Evaluation of the effects of the combination of BMP‑2‑modified BMSCs and PRP on cartilage defects. Experimental and Therapeutic Medicine, 16, 4569-4577. https://doi.org/10.3892/etm.2018.6776
MLA
Ruan, S., Deng, J., Yan, L., Huang, W."Evaluation of the effects of the combination of BMP‑2‑modified BMSCs and PRP on cartilage defects". Experimental and Therapeutic Medicine 16.6 (2018): 4569-4577.
Chicago
Ruan, S., Deng, J., Yan, L., Huang, W."Evaluation of the effects of the combination of BMP‑2‑modified BMSCs and PRP on cartilage defects". Experimental and Therapeutic Medicine 16, no. 6 (2018): 4569-4577. https://doi.org/10.3892/etm.2018.6776