Use of a novel sonosensitizer in sonodynamic therapy of U251 glioma cells in vitro

  • Authors:
    • Zhiqiang Chen
    • Jianhua Li
    • Xueming Song
    • Zhi Wang
    • Wu Yue
  • View Affiliations

  • Published online on: November 25, 2011     https://doi.org/10.3892/etm.2011.390
  • Pages: 273-278
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Abstract

The aim of the present study was to investigate the effect of ZnPcS2P2-meditated sonodynamic therapy (SDT) on U251 human glioma cells and to identify its underlying biological mechanism. The growth inhibition rate was determined by MTT assay. The apoptotic rate was examined by flow cytometry. Fine structures were observed with transmission electron microscopy (TEM). Generation of reactive oxygen species (ROS) was detected spectrophotometrically. Caspase-3, -8 and -9 expression was detected by Western blot analysis. The growth inhibition rate of U251 human glioma cells indicated that ZnPcS2P2-meditated SDT had a better growth inhibition rate of tumor cells at a concentration of 5.0 µg/ml ZnPcS2P2, at a 4-h incubation time with ZnPcS2P2, and at 6 h re-incubation following SDT. At 6 h after SDT, the growth inhibition rate of cells was significantly higher compared to other groups, apoptosis could be detected in SDT by flow cytometry. TEM examination revealed morphological features of apoptosis or necrosis. Furthermore, caspase-3, -8 and -9 expression following SDT was found to be increased by Western blot analysis. Finally, generation of ROS in cells was also elevated. In conclusion, ZnPcS2P2-SDT is capable of inducing U251 cell apoptosis or necrosis and has satisfying antitumor effects. The mechanism of ZnPcS2P2‑meditated SDT involves ROS generation in U251 cells, which initiates subsequent apoptosis through the mitochondrial and death receptor pathways.
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February 2012
Volume 3 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Chen Z, Li J, Song X, Wang Z and Yue W: Use of a novel sonosensitizer in sonodynamic therapy of U251 glioma cells in vitro. Exp Ther Med 3: 273-278, 2012.
APA
Chen, Z., Li, J., Song, X., Wang, Z., & Yue, W. (2012). Use of a novel sonosensitizer in sonodynamic therapy of U251 glioma cells in vitro. Experimental and Therapeutic Medicine, 3, 273-278. https://doi.org/10.3892/etm.2011.390
MLA
Chen, Z., Li, J., Song, X., Wang, Z., Yue, W."Use of a novel sonosensitizer in sonodynamic therapy of U251 glioma cells in vitro". Experimental and Therapeutic Medicine 3.2 (2012): 273-278.
Chicago
Chen, Z., Li, J., Song, X., Wang, Z., Yue, W."Use of a novel sonosensitizer in sonodynamic therapy of U251 glioma cells in vitro". Experimental and Therapeutic Medicine 3, no. 2 (2012): 273-278. https://doi.org/10.3892/etm.2011.390