Fibroblast growth factor 2 inhibits the expression of stromal cell-derived factor 1α in periodontal ligament cells derived from human permanent teeth in vitro

Asakawa,Takeyoshi; Chosa,Naoyuki; Yoshimura,Yoshitaka; Asakawa,Asami; Tanaka,Mitsuro; Ishisaki,Akira; Mitome,Masato; Hasegawa,Tomokazu

doi:10.3892/ijmm.2011.869

Fibroblast growth factor 2 inhibits the expression of stromal cell-derived factor 1α in periodontal ligament cells derived from human permanent teeth in vitro

Authors:
- Takeyoshi Asakawa
- Naoyuki Chosa
- Yoshitaka Yoshimura
- Asami Asakawa
- Mitsuro Tanaka
- Akira Ishisaki
- Masato Mitome
- Tomokazu Hasegawa
View Affiliations

Affiliations: Department of Pediatric Dentistry, School of Dentistry, Iwate Medical University, Morioka 020-8505, Japan, Department of Pediatric Dentistry, Tokushima University Hospital, 3-18-15 Kuramoto, Tokushima 770-8504, Japan
Published online on: December 23, 2011 https://doi.org/10.3892/ijmm.2011.869
Pages: 569-573

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Although cells derived from periodontal ligament (PDL) tissue are reported to have stem cell-like activity and are speculated to play a crucial role for tissue healing and regeneration after injury or orthodontic treatment, mechanisms regulating their recruitment and activation remain unknown. Recently, stromal cell-derived factor 1α (SDF-1α) has been reported to be important for stem cell homing and recruitment to injured sites. The aim of this study was to evaluate whether fibroblast growth factor 2 (FGF-2) affects the expression of SDF-1α in PDL cells derived from human permanent teeth in vitro. Using real-time PCR, the expression of SDF-1α mRNA in PDL cells was inhibited by treatment with 10 ng/ml FGF-2. When PDL cells were treated with SU5402 (an inhibitor of FGF receptor 1) in combination with FGF-2, the FGF-2-reduced expression of SDF-1α was inhibited. In the presence of the JNK inhibitor SP600125, SDF-1α mRNA in PDL cells was not suppressed by the FGF-2 treatment. Western blot analysis also showed that SDF-1α production was suppressed by treatment with FGF-2, but it recovered with treatment by FGF-2 + SU5402. These findings suggest that SDF-1α from PDL cells plays an important role in the regeneration and homeostasis of periodontal tissues via the recruitment of stem cells.

View Figures

View References

1	E FreemanPeriodontiumOral Histology: Development, Structure and FunctionAR Ten CateMosbySt Louis2763121994
2	MC GroeneveldV EvertsW BeertsenAlkaline phosphatase activity in the periodontal ligament and gingiva of the rat molar: its relation to cementum formationJ Dent Res7413741381199510.1177/002203459507400709017560388
3	W BeertsenT van den BosAlkaline phosphatase induces the mineralization of sheets of collagen implanted subcutaneously in the ratJ Clin Invest8919741980199210.1172/JCI1158051602003
4	BM SeoM MiuraS GronthosPM BartoldS BatouliJ BrahimM YoungPG RobeyCY WangS ShiInvestigation of multipotent postnatal stem cells from human periodontal ligamentLancet364149155200410.1016/S0140-6736(04)16627-015246727
5	J WangR LobergRS TaichmanThe pivotal role of CXCL12 (SDF-1)/CXCR4 axis in bone metastasisCancer Metastasis Rev25573587200610.1007/s10555-006-9019-x17165132
6	A ZerneckeA SchoberI BotP von HundelshausenEA LiehnB MöppsM MericskayP GierschikEA BiessenC WeberSDF-1alpha/CXCR4 axis is instrumental in neointimal hyperplasia and recruitment of smooth muscle progenitor cellsCirc Res96784791200510.1161/01.RES.0000162100.52009.3815761195
7	M KuciaJ RatajczakR RecaA Janowska-WieczorekMZ RatajczakTissue-specific muscle, neural and liver stem/progenitor cells reside in the bone marrow, respond to an SDF-1 gradient and are mobilized into peripheral blood during stress and tissue injuryBlood Cells Mol Dis325257200410.1016/j.bcmd.2003.09.025
8	MZ RatajczakM KuciaR RecaM MajkaA Janowska-WieczorekJ RatajczakStem cell plasticity revisited: CXCR4-positive cells expressing mRNA for early muscle, liver and neural cells 'hide out' in the bone marrowLeukemia182940200410.1038/sj.leu.240318414586476
9	S MurakamiPeriodontal tissue regeneration by signaling molecule(s): what role does basic fibroblast growth factor (FGF-2) have in periodontal therapy?Periodontology56188208201110.1111/j.1600-0757.2010.00365.x21501244
10	T HasegawaY YoshimuraT KikuiriY YawakaS TakeyamaA MatsumotoH OguchiT ShirakawaExpression of receptor activator of NF-kappa B ligand and osteoprotegerin in culture of human periodontal ligament cellsJ Periodont Res37405411200210.1034/j.1600-0765.2002.01603.x12472833
11	T HasegawaT KikuiriS TakeyamaY YoshimuraM MitomeH OguchiT ShirakawaHuman periodontal ligament cells derived from deciduous teeth induce osteoclastogenesis in vitroTissue Cell344451200210.1054/tice.2002.022311989970
12	T HasegawaN ChosaT AsakawaY YoshimuraA AsakawaA IshisakiM TanakaEffect of fibroblast growth factor-2 on periodontal ligament cells derived from human deciduous teeth in vitroExp Ther Med1337341201022993547
13	T HasegawaN ChosaT AsakawaY YoshimuraA AsakawaA IshisakiM TanakaEffect of fibroblast growth factor-2 on dental pulp cells derived from human deciduous teeth in vitroExp Ther Med14774802010
14	O TrubianiA IsgroN ZiniI AntonucciF AiutiR Di PrimioA NanciS CaputiR PaganelliFunctional interleukin-7/interleukin-7Ralpha, and SDF-1alpha/CXCR4 are expressed by human periodontal ligament derived mesenchymal stem cellsJ Cell Physiol214706713200810.1002/jcp.2126617894415
15	K NagatomoM KomakiI SekiyaY SakaguchiK NoguchiS OdaT MunetaI IshikawaStem cell properties of human periodontal ligament cellsJ Periodontal Res41303310200610.1111/j.1600-0765.2006.00870.x16827724
16	SC ChoiSJ KimJH ChoiCY ParkWJ ShimDS LimFibroblast growth factor-2 and -4 promote the proliferation of bone marrow mesenchymal stem cells by the activation of the PI3K-Akt and ERK1/2 signaling pathwaysStem Cells Dev17725736200810.1089/scd.2007.023018788932
17	A SchmidtD LadageT SchinkötheU KlausmannC UlrichsFJ KlinzK BrixiusS ArnholdB DesaiU MehlhornBasic fibroblast growth factor controls migration in human mesenchymal stem cellsStem Cells2417501758200610.1634/stemcells.2005-019116822883
18	L JiangYQ ZhuR DuYX GuL XiaF QinHH RitchieThe expression and role of stromal cell-derived factor-1alpha-CXCR4 axis in human dental pulpJ Endod34939944200810.1016/j.joen.2008.05.01518634924
19	Y HosokawaI HosokawaK OzakiH NakaeK MurakamiY MiyakeT MatsuoCXCL12 and CXCR4 expression by human gingival fibroblasts in periodontal diseaseClin Exp Immunol141467474200510.1111/j.1365-2249.2005.02852.x16045736