Icariin promotes bone formation via the BMP-2/Smad4 signal transduction pathway in the hFOB 1.19 human osteoblastic cell line

Liang,Wenna; Lin,Munan; Li,Xihai; Li,Candong; Gao,Bizheng; Gan,Huijuan; Yang,Zhaoyang; Lin,Xuejuan; Liao,Linghong; Yang,Min

doi:10.3892/ijmm.2012.1079

Icariin promotes bone formation via the BMP-2/Smad4 signal transduction pathway in the hFOB 1.19 human osteoblastic cell line

Authors:
- Wenna Liang
- Munan Lin
- Xihai Li
- Candong Li
- Bizheng Gao
- Huijuan Gan
- Zhaoyang Yang
- Xuejuan Lin
- Linghong Liao
- Min Yang
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Affiliations: Research Base of Traditional Chinese Medicine Syndrome, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, P.R. China, Department of Traditional Chinese Medicine, Fuzhou General Hospital of Nanjing Military Command, Fuzhou 350025, P.R. China, Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, P.R. China, Research Base of Traditional Chinese Medicine Syndrome, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, P.R. China
Published online on: July 26, 2012 https://doi.org/10.3892/ijmm.2012.1079
Pages: 889-895

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Abstract

Icariin, the main active compound of the traditional Chinese medicine, Epimedium, is commonly used for the clinical treatment of osteoporosis. However, the precise molecular mechanism of the therapeutic effect of icariin has not been elucidated. The aim of this study was to examine the effect of icariin on cell viability, alkaline phosphatase (ALP) activity, the amount of calcified nodules, and to delineate the molecular mechanism of icariin-enhanced bone formation by investigating the expression of bone morphogenic protein‑2 (BMP-2), Smad4, Cbfa1/Runx2, osteoprotegerin (OPG), receptor activator of nuclear factor κ-B ligand (RANKL) and the OPG/RANKL ratio in the hFOB 1.19 human osteoblastic cell line. We found that icariin significantly increased the cell viability, the activity of ALP and the amount of calcified nodules in the hFOB 1.19 cells. Furthermore, we observed that icariin upregulated the expression of BMP-2, Smad4, Cbfa1/Runx2, OPG, RANKL and the OPG/RANKL ratio. Our results indicate that icariin can modulate the process of bone formation via the BMP-2/Smad4 signal transduction pathway in hFOB 1.19 cells.

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