Effects of histone deacetylase inhibition on the survival, proliferation and migration of Schwann cells, as well as on the expression of neurotrophic factors and genes associated with myelination

Wang,Yazhou; Wu,Xingjun; Zhong,Yueping; Shen,Jianhong; Wu,Xinhua; Ju,Shaoqing; Wang,Xiaofei

doi:10.3892/ijmm.2014.1792

Effects of histone deacetylase inhibition on the survival, proliferation and migration of Schwann cells, as well as on the expression of neurotrophic factors and genes associated with myelination

Authors:
- Yazhou Wang
- Xingjun Wu
- Yueping Zhong
- Jianhong Shen
- Xinhua Wu
- Shaoqing Ju
- Xiaofei Wang
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Affiliations: Laboratory Medicine Center, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China, Department of Neurology, Xuhui Central Hospital, Xuhui, Shanghai 200031, P.R. China, Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China, Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China
Published online on: June 2, 2014 https://doi.org/10.3892/ijmm.2014.1792
Pages: 599-605

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Abstract

Trichostatin A (TSA), a histone deacetylase (HDAC) inhibitor, has been shown to have neuroprotective, neurotrophic and anti-inflammatory properties in both animal and cellular models of neurodegenerative disorders. In a previous study of ours, we demonstrated that TSA inhibited the proliferation and increased the differentiation of neuronal precursor cells (NPCs). However, the effects of TSA on Schwann cells (SCs) have not yet been fully elucidated. Thus, in the present study, using SCs derived from adult rat sciatic nerves, we investigated the effects of TSA on the survival, proliferation, migration and myelination of SCs. We found that TSA significantly induced SC death when used at high concentrations. We also observed that TSA promoted the proliferation of SCs in a time-dependent manner. In addition, TSA inhibited the migration of SCs. Moreover, RT-PCR revealed that TSA increased the mRNA expression of several neurotrophic factors and inhibited the expression of genes associated with myelination, including myelin basic protein (MBP) and myelin protein zero (MPZ). Taken together, our results suggest that TSA plays an important role in regulating the growth and biological function of SCs. These data may contribute to our understanding of TSA-based treatment of neurodegenerative diseases.

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