Profile of microRNAs associated with aging in rat liver

Mimura,Shima; Iwama,Hisakazu; Kato,Kiyohito; Nomura,Kei; Kobayashi,Mitsuyoshi; Yoneyama,Hirohito; Miyoshi,Hisaaki; Tani,Joji; Morishita,Asahiro; Himoto,Takashi; Deguchi,Akihiro; Nomura,Takako; Sakamoto,Teppei; Fujita,Koji; Maeda,Emiko; Izuishi,Kunihiko; Okano,Keiichi; Suzuki,Yasuyuki; Masaki,Tsutomu

doi:10.3892/ijmm.2014.1892

Profile of microRNAs associated with aging in rat liver

Authors:
- Shima Mimura
- Hisakazu Iwama
- Kiyohito Kato
- Kei Nomura
- Mitsuyoshi Kobayashi
- Hirohito Yoneyama
- Hisaaki Miyoshi
- Joji Tani
- Asahiro Morishita
- Takashi Himoto
- Akihiro Deguchi
- Takako Nomura
- Teppei Sakamoto
- Koji Fujita
- Emiko Maeda
- Kunihiko Izuishi
- Keiichi Okano
- Yasuyuki Suzuki
- Tsutomu Masaki
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Affiliations: Department of Gastroenterology and Neurology, Faculty of Medicine, Kagawa University, Kagawa 761-0793, Japan, Department of Signal Transduction Sciences, Faculty of Medicine, Kagawa University, Kagawa 761-0793, Japan, Department of Gastroenterological Surgery, Faculty of Medicine, Kagawa University, Kagawa 761-0793, Japan
Published online on: August 11, 2014 https://doi.org/10.3892/ijmm.2014.1892
Pages: 1065-1072

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Abstract

Recent studies suggest that small non‑coding microRNAs (miRNAs or miRs) play an important role in the regulation of genes involved in various cellular and developmental processes. However, the expression of miRNAs during the aging process remains largely unknown. The aim of the present study was to analyze miRNA expression profiles in rat livers during the aging process. The livers of male Wistar rats at different stages of development (fetal, aged 3 days, and 1, 2, 4, 8 and 36 weeks of age) were used. Total RNA was extracted from the livers. We analyzed the expression levels of 679 rat miRNA probes. In addition, immunohistochemical staining for proliferating cell nuclear antigen (PCNA) was performed. Several up- and downregulated miRNAs were identified in the rat livers at 7 different fetal developmental stages and at 36 weeks of age. We observed the upregulation of miR‑29a, miR‑29c, miR‑195 and miR‑497, whereas miR‑301a, miR‑148b-3p, miR‑7a, miR‑93, miR‑106b, miR‑185, miR‑450a, miR‑539 and miR‑301b were downregulated in the aging rat livers. The number of PCNA-positive hepatocytes was decreased with age. In conclusion, our findings suggest that these up- and downregulated miRNAs play an important role in aging by regulating cell cycles that are involved in liver senescence. Further investigation is required to reveal additional target genes of the miRNAs expressed in the liver and the roles of miRNAs in the developmental process of aging in the liver.

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