Anti-allergic effects of a nonameric peptide isolated from the intestine gastrointestinal digests of abalone (Haliotis discus hannai) in activated HMC-1 human mast cells

Ko,Seok-Chun; Lee,Dae-Sung; Park,Won  Sun; Yoo,Jong  Su; Yim,Mi-Jin; Qian,Zhong-Ji; Lee,Chang-Min; Oh,Junghwan; Jung,Won-Kyo; Choi,Il-Whan

doi:10.3892/ijmm.2015.2420

Anti-allergic effects of a nonameric peptide isolated from the intestine gastrointestinal digests of abalone (Haliotis discus hannai) in activated HMC-1 human mast cells

Authors:
- Seok-Chun Ko
- Dae-Sung Lee
- Won Sun Park
- Jong Su Yoo
- Mi-Jin Yim
- Zhong-Ji Qian
- Chang-Min Lee
- Junghwan Oh
- Won-Kyo Jung
- Il-Whan Choi
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Affiliations: Marine-Integrated Bionics Research Center, Pukyong National University, Busan, Republic of Korea, Converging Research Division, National Marine Biodiversity Institute of Korea, Seochun, Chungcheongnam-do, Republic of Korea, Department of Physiology, Kangwon National University School of Medicine, Chuncheon, Gangwon, Republic of Korea, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, Guangdong, P.R. China, Department of Molecular Microbiology and Immunology, Warren Alpert School of Medicine Box G-L, Providence, RI, USA
Published online on: November 27, 2015 https://doi.org/10.3892/ijmm.2015.2420
Pages: 243-250

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Abstract

The aim of the present study was to examine whether the intestine gastrointestinal (GI) digests of abalone [Haliotis discus hannai (H. discus hannai)] modulate inflammatory responses and to elucidate the mechanisms involved. The GI digests of the abalone intestines were fractionated into fractions I (>10 kDa), II (5-10 kDa) and Ⅲ (<5 kDa). Of the abalone intestine GI digests (AIGIDs), fraction Ⅲ inhibited the passive cutaneous anaphylaxis (PCA) reaction in mice. Subsequently, a bioactive peptide [abalone intestine GI digest peptide (AIGIDP)] isolated from fraction Ⅲ was determined to be 1175.2 Da, and the amino acid sequence was found to be PFNQGTFAS. We noted that the purified nonameric peptide (AIGIDP) attenuated the phorbol‑12‑myristate 13-acetate plus calcium ionophore A23187 (PMACI)-induced histamine release and the production of pro-inflammatory cytokines, such as tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 in human mast cells (HMC-1 cells). In addition, we also noted that AIGIDP inhibited the PMACI‑induced activation of nuclear factor‑κB (NF-κB) by suppressing IκBα phosphorylation and that it suppressed the production of cytokines by decreasing the phosphorylation of JNK. The findings of our study indicate that AIGIDP exerts a modulatory, anti-allergic effect on mast cell-mediated inflammatory diseases.

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