Open Access

A novel method of neural differentiation of PC12 cells by using Opti-MEM as a basic induction medium

  • Authors:
    • Rendong Hu
    • Qiaoyu Cao
    • Zhongqing Sun
    • Jinying Chen
    • Qing Zheng
    • Fei Xiao
  • View Affiliations

  • Published online on: October 19, 2017     https://doi.org/10.3892/ijmm.2017.3195
  • Pages:195-201
  • Copyright: © Hu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The PC12 cell line is a classical neuronal cell model due to its ability to acquire the sympathetic neurons features when deal with nerve growth factor (NGF). In the present study, the authors used a variety of different methods to induce PC12 cells, such as Opti-MEM medium containing different concentrations of fetal bovine serum (FBS) and horse serum compared with RPMI-1640 medium, and then observed the neurite length, differentiation, adhesion, cell proliferation and action potential, as well as the protein levels of axonal growth-associated protein 43 (GAP-43) and synaptic protein synapsin-1, among other differences. Compared with the conventional RPMI-1640 medium induction method, the new approach significantly improved the neurite length of induced cells (2.7 times longer), differentiation rate (30% increase), adhesion rate (21% increase) and expression of GAP-43 and synapsin-1 (three times), as well as reduced cell proliferation. The morphology of induced cells in Opti-MEM medium containing 0.5% FBS was more like that of neurons. Additionally, induced cells were also able to motivate the action potential after treatment for 6 days. Therefore, the research provided a novel, improved induction method of neural differentiation of PC12 cells using Opti-MEM medium containing 0.5% FBS, resulting in a better neuronal model cell line that can be widely used in neurobiology and neuropharmacology research.

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January 2018
Volume 41 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

2016 Impact Factor: 2.341
Ranked #21/128 Medicine Research and Experimental
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APA
Hu, R., Cao, Q., Sun, Z., Chen, J., Zheng, Q., & Xiao, F. (2018). A novel method of neural differentiation of PC12 cells by using Opti-MEM as a basic induction medium. International Journal of Molecular Medicine, 41, 195-201. https://doi.org/10.3892/ijmm.2017.3195
MLA
Hu, R., Cao, Q., Sun, Z., Chen, J., Zheng, Q., Xiao, F."A novel method of neural differentiation of PC12 cells by using Opti-MEM as a basic induction medium". International Journal of Molecular Medicine 41.1 (2018): 195-201.
Chicago
Hu, R., Cao, Q., Sun, Z., Chen, J., Zheng, Q., Xiao, F."A novel method of neural differentiation of PC12 cells by using Opti-MEM as a basic induction medium". International Journal of Molecular Medicine 41, no. 1 (2018): 195-201. https://doi.org/10.3892/ijmm.2017.3195